Oxidative stress in lung tissue induced by CO2 pneumoperitoneum in the rat

Pross, M.; Schulz, Hans–Ulrich; Flechsig, Antje; Mussack, Thomas; Halangk, Walter; Augustin, Wolfgang; Lippert, H.; Reinheckel, Thomas

doi:10.1007/s004640000189

Cited by 37 publications

(35 citation statements)

References 14 publications

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“…Increased MDA levels of the lung homogenates at 2 and 6 h and control levels at 18 h. Almost no difference in protein carbonyl content. Decreased reduced glutathione levels in the lung tissue at 2 h after laparoscopy with control levels at 18 h after laparoscopy in rats [19]. Vecchio et al evaluated the coagulation pathway assessing prothrombin time, activated partial thromboplastin time, prothrombin fragment F1 ?…”

Section: Discussionmentioning

confidence: 99%

The Effect of Carbon Dioxide Insufflation Applied at Different Pressures and Periods on Thrombotic Factors

Sen

Türkyılmaz

Sönmez

et al. 2015

Indian J Hematol Blood Transfus

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The aim of this experimantal study which is applied on rats, is to determine the differences on the clotting factors over the application of low and high intraabdominal pressure (IAP) values in different periods of time in carbon dioxide (CO 2 ) pneumoperitoneum. Thirty rats were randomized into five groups (n = 6): a control group (Group K) and 1 h and 6 mm Hg IAP (Group A), 2 h and 6 mm Hg IAP (Group B), 1 h and 12 mm Hg IAP (Group C) and 2 h and 12 mm Hg IAP were created with CO 2 pneumoperitoneum (Group D). At the end of the experiment, plasma samples taken from subjects and fibrinogen, FII (prothrombin), FV, FVII, FVIII, FIX, FX, FXI, FXII, von willebrand's factor (vWF), ristocetin cofactor, protein C, protein S, antithrombin III (AT III) levels are studied. There were statistically significant differences in the mean levels of FII, FV, FVII, FVIII, FIX, FX, FXI, FXII, and protein S between the groups. A hypercoagulable state occurred with the following: increase in the coagulation parameters compared to the control group; increase in FVII in the group only Group C; decrease in AT III in all groups compared to the control group; decrease in protein C in the group only XII Group D compared to control group; decrease in protein S in all groups except group D compared to control group. CO 2 insufflation predisposes to thromboembolic events both by inducing coagulation factors and by suppressing the fibrinolytic system contrary to the controversies in the literature.

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Section: Discussionmentioning

confidence: 99%

The Effect of Carbon Dioxide Insufflation Applied at Different Pressures and Periods on Thrombotic Factors

Sen

Türkyılmaz

Sönmez

et al. 2015

Indian J Hematol Blood Transfus

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“…Ischemia and reperfusion induce the release of inflammatory mediators that lead to lipid peroxidation and produce a series of oxygen-derived free radicals 21,22 . This not only makes the injury worse, but also generates further tissue changes (14).…”

Section: Effects Of Carbon Dioxide Pneumoperitoneum On Hepatic and Rementioning

confidence: 99%

Effects of carbon dioxide pneumoperitoneum on hepatic and renal morphology of rats after segmental colectomy and colonic anastomosis

et al. 2011

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PURPOSE: To evaluate the effects of increased intraperitoneal pressure caused by carbon dioxide pneumoperitoneum on the hepatic and renal morphology of rats. METHODS: Fifty-four adult male rats were randomly divided into three groups (P, PP and C) after anesthesia: P - in 18 animals, pneumoperitoneum was established for 30 minutes immediately before laparotomy; PP - in 18 animals, pneumoperitoneum was established for 60 minutes divided into 30 immediately before laparotomy and 30 after abdominal closure; control group (C) - 18 animals underwent laparotomy without pneumoperitoneum induction. The pneumoperitoneum was maintained at a pressure of 5 mm Hg. Nine animals in each group were killed on the 3rd and 7th postoperative days, when kidney and liver samples were collected for morphological analysis. The liver specimens were stained with hematoxylin and eosin (HE), and the kidney specimens, with HE and von Kossa. Blinded examiners analyzed the slides. RESULTS: No changes in renal morphology were found. Liver samples showed histological signs of degeneration in animals in the pneumoperitoneum groups killed on the 7th postoperative day (p=0.029). CONCLUSION: The CO2 pneumoperitoneum did not affect renal morphology but caused hydropic degeneration in the liver of animals killed on the 7th postoperative day.

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“…HNE is a major α, β-unsaturated aldehyde end product derived from peroxidation of ω-6 polyunsaturated fatty acids such as linoleic acid and arachidonic acid. The concentration of HNE is markedly increased under conditions of oxidative stress [16][17][18][19][20][21][22]. The biologic significance of HNE was first recognized to be as a secondary toxicant that was responsible for the "action at a distance"-damage caused by free radicals [23,24] and as a biomarker of oxidative stress [25].…”

Section: Introductionmentioning

confidence: 99%

γ-Glutamyl transpeptidase is induced by 4-hydroxynonenal via EpRE/Nrf2 signaling in rat epithelial type II cells

Zhang

Liu

Dickinson

et al. 2006

Free Radical Biology and Medicine

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Abstractγ-Glutamyl transpeptidase (GGT) plays key roles in glutathione homeostasis and metabolism of glutathione S-conjugates. Rat GGT is transcribed via five tandemly arranged promoters into seven transcripts. The transcription of mRNAV is controlled by promoter 5. Previously we found that GGT mRNAV-2 was responsible for the induction of GGT in rat alveolar epithelial cells by 4-hydroxynonenal (HNE). In the current study, the underlying mechanism was investigated. Reporter deletion and mutation analysis demonstrated that an electrophile-response element (EpRE) in the proximal region of GGT promoter 5 (GP5) was responsible for the basal-and HNE-induced promoter activity. Gel-shift assays showed an increased binding activity of GP5 EpRE after HNE exposure. The nuclear content of NF-E2-related factor 2 (Nrf2) was significantly increased by HNE. The recruitment of Nrf2 to GP5 EpRE after HNE treatment was demonstrated by supershift and chromatin immunoprecipitation assays. The tissue expression pattern of GGT mRNA V was previously unknown. Using polymerase chain reaction, we found that GGT mRNAV-2 was expressed in many tissues in rat. Taken together, GGT mRNAV-2 is widely expressed in rat tissues and its basal and HNE-induced expression is mediated through EpRE/Nrf2 signaling.

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Oxidative stress in lung tissue induced by CO2 pneumoperitoneum in the rat

Abstract: Oxidative stress is likely to contribute to the impairment of pulmonary function after laparoscopic operations using a CO(2) pneumoperitoneum.

Cited by 37 publications

References 14 publications

The Effect of Carbon Dioxide Insufflation Applied at Different Pressures and Periods on Thrombotic Factors

The Effect of Carbon Dioxide Insufflation Applied at Different Pressures and Periods on Thrombotic Factors

Effects of carbon dioxide pneumoperitoneum on hepatic and renal morphology of rats after segmental colectomy and colonic anastomosis

γ-Glutamyl transpeptidase is induced by 4-hydroxynonenal via EpRE/Nrf2 signaling in rat epithelial type II cells

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