Oxidative stress in diseases of the human cornea

Shoham, Akiva; Hadziahmetovic, Majda; Dunaief, Joshua L.; Mydlarski, Marc B.; Schipper, Hyman M.

doi:10.1016/j.freeradbiomed.2008.07.021

Cited by 169 publications

(118 citation statements)

References 87 publications

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“…During HBO, the excess production of reactive oxygen, nitrogen species and lack of antioxidant supplements can result in pathological oxidative stress, affecting the enzymatic antioxidant defense system [11]. Hence, prolonged HBO treatment can cause failure in the antioxidant defense system, leading to oxidative damages to ocular structures and cause the development of myopia, nuclear cataracts and keratoconus [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Neovascularization on Human Retinal Pigment Epithelium after Hyperbaric Oxygen Therapy

Chen¹,

Chao²

2017

Health Sci J

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Objective: To investigate the neovascularization effect of hyperbaric oxygen (HBO) therapy on human retinal pigment epithelium (REP) cells. Methods:Human REP cells were cultured in vitro, and divided into normoxic control group and hypoxic control group randomly. The experimental groups were then divided into three subgroups via the different HBO pressure, 0.15 MPa exposed group, 0.2 MPa exposed group, and 0.25 MPa exposed group. The experimental groups were exposed to 100% pure oxygen for 60 and 90 minutes twice, with a 24 hours interval. After HBO exposure the expression of pigment epithelium-derived factor (PEDF) and tumor necrosis factor alpha (TNF-α) by RPE cells were measured by western blot. Results:The western blot result showed that the level of PEDF expression in both experimental groups had an increased trend as the pressure increases with statistical significance (P<0.01). The expression of PEDF in the normoxic group was higher than the hypoxic group. The results showed that the level of TNF-α expression by RPE cells in both experimental groups have a decrease trend as the pressure increases with statistical significance (P<0.01). The results showed that the expression of TNF-α in the hypoxic group was higher when compared to the normoxic group. The duration of HBO exposure has no statistical significance on the level of PEDF and TNF-α expression. Conclusion:This study supports the idea that HBO increases the release of PEDF by RPE cells whither in normoxic and hypoxic conditions, and decreases the release of TNF-α by RPE cells in both conditions, especially in hypoxic state. We also found that HBO can raise the level of PEDF and decrease the level of TNF-α in RPE cells, and has an anti-angiogenic effect which can suppress the formation of neovascularization.

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Section: Introductionmentioning

confidence: 99%

The Effect of Neovascularization on Human Retinal Pigment Epithelium after Hyperbaric Oxygen Therapy

Chen¹,

Chao²

2017

Health Sci J

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“…4,5 However, keratoconic corneas have accumulation of cytotoxic byproducts (such as superoxides, hydrogen peroxide, and hydroxyl radicals) and abnormalities in antioxidant defense mechanisms. [6][7][8] More recently, corneal thinning in the keratoconus has been found to be associated with increase in tissue proteinase activities and decrease in proteinase inhibitor levels induced by oxidant products. 9 Free radicals are produced in metabolic and physiological processes, and harmful oxidative reactions may occur in organisms.…”

Section: Introductionmentioning

confidence: 99%

Increased systemic oxidative stress in patients with keratoconus

Toprak

Küçükatay

Yıldırım

et al. 2013

Eye

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Purpose To establish the effect of systemic oxidative stress on the pathogenesis of keratoconus by measuring serum total oxidant status (TOS) and total antioxidant status (TAS) in patients with keratoconus. Methods Twenty-five patients with keratoconus (keratoconus group) and 25 age-sex-matched healthy subjects (control group) were enrolled in the study. Exclusion criteria were smoking habit, history of any other corneal pathology, systemic disease or inflammation, and current antioxidant or anti-inflammatory therapies. All participants underwent a detailed ophthalmological examination and corneal topography. Serum samples were obtained from all participants. Oxidative stress markers (TAS and TOS) were measured using a commercial kit and oxidative stress index (OSI) was calculated. Results The study comprised 25 patients with keratoconus (mean age of 26.4 ± 1.7 years) and 25 healthy control subjects (mean age of 26.6 ± 1.7 years) (P40.05). The serum TOS and OSI values were significantly higher in patients with keratoconus compared with those of the controls (P ¼ 0.036 and 0.037, respectively). However, serum TAS did not show significant difference between the keratoconus and control groups (P ¼ 0.497). Conclusions The higher levels of serum oxidant status and OSI in patients with keratoconus suggest that systemic oxidative stress might be involved in the pathogenesis of keratoconus.

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“…R edox imbalance between reactive species and endogenous antioxidants, which results in oxidative damage to biologic molecules and impairment in signaling pathways [(i.e., in oxidative stress (145)], has been widely implicated in many ailments, including central nervous system pathologies (46,51,61,122,219,330) [e.g., amyotrophic lateral sclerosis, (46), Parkinson's disease (219), bipolar disorder (330), Alzheimer's disease (61)], cardiovascular conditions (61,112), pulmonary conditions (65,153), diabetes (111,154), eye diseases (19,235), aging (290,323,236), cancer (52,70,317), radiation injury (220), pain=chronic morphine tolerance (89), Fanconi anemia (229). Reactive species, such as nitric oxide ( · NO), superoxide (O 2 ·À ), hydrogen peroxide (H 2 O 2 ), peroxynitrite (ONOO -), and others have been widely recognized as signaling species that, by affecting redox-based cellular transcriptional activity, control inflammatory and immune responses and enhance secondary oxidative stress (27,47,96,151,188,273,276,298,335,336).…”

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confidence: 99%

Superoxide Dismutase Mimics: Chemistry, Pharmacology, and Therapeutic Potential

Batinić‐Haberle

Rebouças

Spasojević

2010

Antioxidants & Redox Signaling

465

689

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Oxidative stress has become widely viewed as an underlying condition in a number of diseases, such as ischemia-reperfusion disorders, central nervous system disorders, cardiovascular conditions, cancer, and diabetes. Thus, natural and synthetic antioxidants have been actively sought. Superoxide dismutase is a first line of defense against oxidative stress under physiological and pathological conditions. Therefore, the development of therapeutics aimed at mimicking superoxide dismutase was a natural maneuver. Metalloporphyrins, as well as Mn cyclic polyamines, Mn salen derivatives and nitroxides were all originally developed as SOD mimics. The same thermodynamic and electrostatic properties that make them potent SOD mimics may allow them to reduce other reactive species such as peroxynitrite, peroxynitrite-derived CO 3 _ À , peroxyl radical, and less efficiently H 2 O 2 . By doing so SOD mimics can decrease both primary and secondary oxidative events, the latter arising from the inhibition of cellular transcriptional activity. To better judge the therapeutic potential and the advantage of one over the other type of compound, comparative studies of different classes of drugs in the same cellular and=or animal models are needed. We here provide a comprehensive overview of the chemical properties and some in vivo effects observed with various classes of compounds with a special emphasis on porphyrin-based compounds. Antioxid. Redox Signal. 13, 877-918.

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Oxidative stress in diseases of the human cornea

Cited by 169 publications

References 87 publications

The Effect of Neovascularization on Human Retinal Pigment Epithelium after Hyperbaric Oxygen Therapy

The Effect of Neovascularization on Human Retinal Pigment Epithelium after Hyperbaric Oxygen Therapy

Increased systemic oxidative stress in patients with keratoconus

Superoxide Dismutase Mimics: Chemistry, Pharmacology, and Therapeutic Potential

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