2012
DOI: 10.4161/auto.21164
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Oxidative stress impairs autophagic flux in prion protein-deficient hippocampal cells

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Cited by 42 publications
(45 citation statements)
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“…These data comply with the findings of other groups demonstrating that autophagy is upregulated in PRNP-deficient cells. 53,54 Moreover, our findings are consistent with a connection between the endocytic pathway, autophagy, and exosome biogenesis and secretion. 33,34 It is well known that autophagosome maturation requires ESCRT function, and that autophagosomes are able to fuse with endocytic vesicles (as MVBs) or lysosomes, which contain the hydrolytic enzymes required to degrade autophagosomal content.…”
Section: Discussionsupporting
confidence: 88%
“…These data comply with the findings of other groups demonstrating that autophagy is upregulated in PRNP-deficient cells. 53,54 Moreover, our findings are consistent with a connection between the endocytic pathway, autophagy, and exosome biogenesis and secretion. 33,34 It is well known that autophagosome maturation requires ESCRT function, and that autophagosomes are able to fuse with endocytic vesicles (as MVBs) or lysosomes, which contain the hydrolytic enzymes required to degrade autophagosomal content.…”
Section: Discussionsupporting
confidence: 88%
“…Perhaps related to SIRT1 inhibition, excess concentrations of glucose and palmitate may also impair autophagy through the generation of oxidative stress (4,8,15,40,72). Reactive oxygen species inhibit the activity of Atg4 (78), an enzyme required both for the maturation of LC3 prior to its incorporation into the forming autophagosomal membrane and for LC3 cleavage from the outside of the autolysosome to be recycled for future use (46).…”
Section: Discussionmentioning
confidence: 99%
“…Western blotting was performed as described previously [27,28]. Briefly, cells were lysed in lysis buffer with protease inhibitors cocktail and phosphatase inhibitor cocktail.…”
Section: Methodsmentioning
confidence: 99%