Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death

Abstract

Metal oxide nanoparticles (NPs) have shown enhanced antibacterial effects against many bacteria. Thus, understanding the potential antibacterial effects of nickel oxide nanoparticles (NiO NPs) against Grampositive and Gram-negative pathogenic bacteria is an urgent need to enable the exploration of NiO NP use in biomedical sciences. To this end, NiO NPs were synthesized by microwave assisted hydrothermal synthesis method. The synthesized NPs were characterized by X-ray diffraction (XRD) and Fourier Transfer Infrared (FT-IR) and UV-visible spectroscopy. The morphological features of the synthesized NiO NPs were analysed using Transmission Electron Microscopy (TEM) and FE-SEM analysis. The antibacterial activity of NiO NP was explored using different antimicrobial and biophysical studies. The obtained data reveals that the NiO NP has stronger antibacterial activity against Gram-positive bacteria compared to Gram-negative bacteria. The mechanism behind the antibacterial activity of the NiO NP was explored by evaluating the amount of ROS generation at the NiO NP interface. The effect of ROS generation on the bacterial membrane was evaluated by BacLight assay and morphological analysis of the bacterial membrane using FE-SEM. The data altogether suggested that the oxidative stress generated at the NiO NP interface resulted in membrane damage leading to bacterial cell death.

A comparative investigation of normal and inverted exchange bias effect for magnetic fluid hyperthermia applications

“…The average dimensions of the rod-shaped sample N were observed as length of 34.13 ± 3 nm and width of 10.97 ± 2 nm. The rod-shaped morphology of NiO NPs has been reported by researchers and the present sample N possess similar morphology to previously reported literature 47 . For the CS samples, the observed average particle sizes were 31.75 ± 3 nm for sample FN and 36.44 ± 2 nm for sample NF.…”

Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis

“…Nanotechnology and its microscopic universe offer unlimited possibilities for contemporary science and various branches of industry. One of the benefits of modern nanotechnology is the production of nanomaterials (NMs) with antimicrobial activity and implementing them into different commercial processes and products as a defence against multidrug resistant infectious microorganisms [1][2][3]. Direct manipulation of synthesised NMs and adequate alteration of their properties can create broad-spectrum and long-term antimicrobials, which is a substantial trait, especially in the medical field.…”

“…ROS-induced stress in microbial cells can damage cellular structures and outer layers, including oxidative degradation of proteins and lipids [2,17]. Interestingly, each type of ROS is characterised by its specific reactivity.…”

In-situ Synthesis rGO/NiO Nanorod Nanocomposites Via One-Step Hydrothermal Method and Investigating Physical Properties of Nanocomposites

“…Niken oxit (NiO) là vật liệu bán dẫn loại p với bề rộng vùng cấm lớn ~3.6 -4.0 eV. NiO là một vật liệu hứa hẹn trong nhiều lĩnh vực nghiên cứu khác nhau vì những ưu điểm vượt trội của nó như trong cảm biến khí [1][2][3], vật liệu quang xúc tác cho phản ứng phân hủy các thuốc nhuộm công nghiệp [4][5][6], chế tạo pin năng lượng mặt trời [7], chất phủ điện sắc [8,9], diệt khuẩn [10][11][12]… Graphen (Gr) là một dạng hợp chất cấu tạo từ đơn lớp các nguyên tử Carbon, đã được nghiên cứu rất nhiều do các tính chất nổi bật như diện tích bề mặt ISSN: 2734-9381 https://doi.org/10.51316/jst.149.etsd.2021.31.2.20 Received: August 30, 2020; accepted: December 23, 2020 lớn [13], độ linh động điện tử vượt trội [14], dẫn nhiệt tốt và độ bền cao. Tuy nhiên, do hiệu ứng biên, khả năng phân tán thấp và khả năng dễ bị tái xếp lớp nên ứng dụng của Gr còn bị hạn chế.…”