Oxidative Stress, Endogenous Antioxidant and Ischemia-modified Albumin in Normolipidemic Acute Myocardial Infarction Patients

Abstract

Dyslipidemia appears to be a conventional risk factor for acute myocardial infarction (AMI). Several studies have reported decreased levels of antioxidants and increased levels of ischemia-modified albumin (IscMA) and lipid peroxides in dyslipidemic myocardial infarct patients. However, literature search reveals no reports of normolipidemic AMI patients with reference to antioxidants and IscMA studies. Therefore, this study determined the endogenous levels of antioxidants and IscMA in normolipidemic AMI patients so that prospective measures could be taken to avoid acute coronary complications. The serum lipid profile, albumin, uric acid, total bilirubin, malondialdehyde, conjugated dienes and IscMA levels were determined in 165 normolipidemic AMI patients and 165 age/sex-matched controls. In addition, serum lipid concentrations were estimated by enzymatic methods. Endogenous antioxidants were significantly decreased in AMI patients compared with controls. In parallel with this, serum malondialdehyde (MDA) and conjugated dienes were significantly increased in AMI patients compared with controls. IscMA levels were significantly increased in AMI patients compared with controls. As for the serum lipid profile, total cholesterol (TC), cholesterol in low density lipoprotein (LDL) and triglycerides (TG) were higher in AMI subjects. High-density lipoprotein (HDL)-to-cholesterol and LDL-cholesterol-to-HDLcholesterol ratios were also greater in AMI subjects. * To whom correspondence should be addressed: Department of However, HDL-cholesterol was lower in AMI patients than control. AMI is a multifactorial disease that can arise even in normolipidemic subjects. The present study suggests that measuring of serum antioxidants and IscMA in normolipidemic patients would provide an index of oxidative stress and ischemia due to structural modifications of circulating albumin in serum.

The Level of Ischemic Modified Albumin (IMA) as Risk Marker for Cardio Vascular D isease (CVD) among some diabetic patients (type II) in Khartoum state -Sudan

“…We found that the Serum levels of IMA were significantly higher in diabetic patients when compared with normal healthy non diabetic (p value = 0.003)this agree with previous study show that the IMA is a novel marker of tissue ischemia and accepted as a marker of oxidative stress in type 2 diabetes patients [15] and others study report that definite and precise mechanisms for IMA production in vivo, it appears to be related to the generation of reactive oxygen species (ROS) due to ischemia-reperfusion that modifies metal binding domains of albumin molecule [16]. Diabetic complications are due to various micro and macroangio-pathic events producing increased oxidative stress and decreased levels of antioxidants, which can lead to modification of albumin molecule of IMA than patients with less than 5 years duration of diabetes(long-stand diabetes) have a lower than the other one [18,17].…”

Evaluation of ischemia-modified albumin, oxidative stress, and antioxidant status in acute ischemic stroke patients

“…Kumar et al . [ 22 ] in their study ‘Oxidative stress, endogenous antioxidant, and IMA in normolipidemic acute myocardial infarction patients’ had shown decrease in endogenous antioxidants like uric acid, albumin, and bilirubin due to oxidative stress with a simultaneous increase in IMA. Albumin is the most abundant serum protein.…”

Study of cord blood ischemia modified albumin levels in the evaluation of birth asphyxia

“…Principle ACB assay for determination of the level of IMA in serum is done by addition of a known amount of cobalt (II) to a serum specimen and measurement of the unbound cobalt (II) from the absorbance of the colored complex between dithiotreitol (DTT) and free cobalt by spectrophotometer which is indicative of the level of IMA [30]. Intensity of the colored complex varies inversely with the ACB.…”