Oxidative stress during recovery from muscle atrophy

Kondo, Hisao; Kodama, Junko; Kishibe, Takako; Itokawa, Yoshinori

doi:10.1016/0014-5793(93)81788-2

Cited by 37 publications

(37 citation statements)

References 12 publications

(12 reference statements)

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“…It is also conceivable that HSP72 can play a protective role in the prevention of muscle protein degradation during periods of reduced contractile activity. The potential mechanistic link between HSP72 and protein degradation is that muscle atrophy induced by immobilization is accompanied by oxidative injury in myocytes (6). This increase in oxidative stress accelerates muscle protein breakdown because oxidatively modified proteins are very susceptible to proteolytic attack (5).…”

Section: Discussionmentioning

confidence: 99%

“…A conceivable link between HSP72 and reduced protein degradation in muscle is as follows. Recent evidence demonstrates that muscle atrophy induced by immobilization is associated with oxidative injury in myocytes (6). This increase in oxidative stress may accelerate muscle protein breakdown because oxidatively modified proteins are more susceptible to proteolytic attack.…”

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confidence: 99%

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Heat stress attenuates skeletal muscle atrophy in hindlimb-unweighted rats

Naito

Powers²,

Demirel³

et al. 2000

Journal of Applied Physiology

216

209

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Heat stress attenuates skeletal muscle atrophy in hindlimbunweighted rats. J. Appl. Physiol. 88: 359-363, 2000.-This study tested the hypothesis that elevation of heat stress proteins by whole body hyperthermia is associated with a decrease in skeletal muscle atrophy induced by reduced contractile activity (i.e., hindlimb unweighting). Female adult rats (6 mo old) were assigned to one of four experimental groups (n ϭ 10/group): 1) sedentary control (Con), 2) heat stress (Heat), 3) hindlimb unweighting (HLU), or 4) heat stress before hindlimb unweighting (HeatϩHLU). Animals in the Heat and HeatϩHLU groups were exposed to 60 min of hyperthermia (colonic temperature ϳ41.6°C). Six hours after heat stress, both the HLU and HeatϩHLU groups were subjected to hindlimb unweighting for 8 days. After hindlimb unweighting, the animals were anesthetized, and the soleus muscles were removed, weighed, and analyzed for protein content and the relative levels of heat shock protein 72 (HSP72). Compared with control and HLU animals, the relative content of HSP72 in the soleus muscle was significantly elevated (P Ͻ 0.05) in both the Heat and HeatϩHLU animals. Although hindlimb unweighting resulted in muscle atrophy in both the HLU and HeatϩHLU animals, the loss of muscle weight and protein content was significantly less (P Ͻ 0.05) in the HeatϩHLU animals. These data demonstrate that heat stress before hindlimb unweighting can reduce the rate of disuse muscle atrophy. We postulate that HSP70 and/or other stress proteins play a role in the control of muscle atrophy induced by reduced contractile activity. non-weight bearing; hindlimb suspension; hyperthermia; heat shock protein 70; protein synthesis; soleus muscle IT IS WELL KNOWN THAT DECREASING the load on a skeletal muscle results in muscle atrophy (1,8,(14)(15)(16). Studies using a rodent model of muscle disuse atrophy (i.e., hindlimb unweighting via tail suspension) indicate that the initial loss of muscle protein is primarily due to a decrease in the rate of protein synthesis (14). Subsequent atrophy then occurs by increased rates of protein degradation (14). It has been suggested that the initial decrease in protein synthesis during non-weightbearing activity is a result of a reduced rate of nascent polypeptide chain elongation at the ribosomal level (7). Because the inducible form of the 70-kDa heat shock protein (HSP72) plays an important role in chaperoning nascent peptides during translation, it has been postulated that a decrease in cellular HSP72 levels in myocytes is a potential mechanism to explain the decreased translation observed during muscle disuse (8). Hence, it is conceivable that elevation of cellular HSP72 levels could serve as a countermeasure to attenuate the disuse-induced reduction in protein synthesis.It also seems possible that HSP72 can play a protective role in the prevention of muscle protein degradation during periods of reduced contractile activity. A conceivable link between HSP72 and reduced protein degradation in muscle is as follows. Recent evid...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Heat stress attenuates skeletal muscle atrophy in hindlimb-unweighted rats

Naito

Powers²,

Demirel³

et al. 2000

Journal of Applied Physiology

216

209

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“…Additionally, due to the comparatively greater numbers of mitochondria contained within them, oxidative fibres produce comparatively larger quantities of reactive oxygen species as a byproduct of normal cell metabolism. Reactive oxygen species, or free radicals, are responsible for stochastically damaging cell architecture, including proteins and lipids, which leads to cellular apoptosis and hence muscle disuse atrophy (Kondo et al, 1993a;Kondo et al, 1993b;Kondo et al, 1993c;Kondo et al, 1994). The dramatic downregulation of activity in the oxidative muscle may trigger profound changes in the biochemical function of the muscle mitochondria.…”

Section: The Journal Of Experimental Biologymentioning

confidence: 99%

Getting the jump on skeletal muscle disuse atrophy: preservation of contractile performance in aestivatingCyclorana alboguttata(Günther 1867)

Symonds

James

Franklin

2007

Journal of Experimental Biology

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SUMMARY Prolonged immobilisation or unloading of skeletal muscle causes muscle disuse atrophy, which is characterised by a reduction in muscle cross-sectional area and compromised locomotory function. Animals that enter seasonal dormancy, such as hibernators and aestivators, provide an interesting model for investigating atrophy associated with disuse. Previous research on the amphibian aestivator Cyclorana alboguttata (Günther 1867)demonstrated an absence of muscle disuse atrophy after 3 months of aestivation, as measured by gastrocnemius muscle contractile properties and locomotor performance. In this study, we aimed to investigate the effect of aestivation on iliofibularis and sartorius muscle morphology and contractile function of C. alboguttata over a longer, more ecologically relevant time-frame of 9 months. We found that whole muscle mass, muscle cross-sectional area, fibre number and proportions of fibre types remained unchanged after prolonged disuse. There was a significant reduction in iliofibularis fibre cross-sectional area (declined by 36% for oxidative fibre area and 39% for glycolytic fibre area) and sartorius fibre density (declined by 44%). Prolonged aestivation had little effect on the isometric properties of the skeletal muscle of C. alboguttata. There was a significant reduction in the isometric contraction times of the relatively slow-twitch iliofibularis muscle, suggesting that the muscle was becoming slower after 9 months of aestivation (time to peak twitch increased by 25%, time from peak twitch to half relaxation increased by 34% and time from last stimulus to half tetanus relation increased by 20%). However, the results of the work-loop analysis clearly demonstrate that, despite changes to muscle morphology and isometric kinetics, the overall contractile performance and power output levels of muscles from 9-month aestivating C. alboguttata are maintained at control levels.

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“…The metabolic profiles of oxidative and glycolytic myofibres are inextricably linked to muscle disuse atrophy because oxidative stress is thought to contribute to atrophy in mammalian muscle (Kondo et al, 1991;Kondo et al, 1993;Kondo et al, 1994). Reactive oxygen species (ROS) are a natural by-product of oxidative metabolism, and in a normal metabolic environment there is a balance between the production of ROS and the protective antioxidants that scavenge or deactivate them (Ames et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Skeletal muscle atrophy occurs slowly and selectively during prolonged aestivation inCyclorana alboguttata(Günther 1867)

Mantle

Hudson

Harper

et al. 2009

Journal of Experimental Biology

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SUMMARYWe investigated the effect of prolonged immobilisation of six and nine months duration on the morphology and antioxidant biochemistry of skeletal muscles in the amphibian aestivator Cyclorana alboguttata. We hypothesised that, in the event of atrophy occurring during aestivation, larger jumping muscles were more likely to be preserved over smaller non-jumping muscles. Whole muscle mass (g), muscle cross-sectional area (CSA) (mm 2 ), water content (%) and myofibre number (per mm 2 ) remained unchanged in the cruralis muscle after six to nine months of aestivation; however, myofibre area (mm 2 ) was significantly reduced. Whole muscle mass, water content, myofibre number and myofibre CSA remained unchanged in the gastrocnemius muscle after six to nine months of aestivation. However, iliofibularis dry muscle mass, whole muscle CSA and myofibre CSA was significantly reduced during aestivation. Similarly, sartorius dry muscle mass, water content and whole muscle CSA was significantly reduced during aestivation. Endogenous antioxidants were maintained at control levels throughout aestivation in all four muscles. The results suggest changes to muscle morphology during aestivation may occur when lipid reserves have been depleted and protein becomes the primary fuel substrate for preserving basal metabolic processes. Muscle atrophy as a result of this protein catabolism may be correlated with locomotor function, with smaller non-jumping muscles preferentially used as a protein source during fasting over larger jumping muscles. Higher levels of endogenous antioxidants in the jumping muscles may confer a protective advantage against oxidative damage during aestivation; however, it is not clear whether they play a role during aestivation or upon resumption of normal metabolic activity.

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Oxidative stress during recovery from muscle atrophy

Cited by 37 publications

References 12 publications

Heat stress attenuates skeletal muscle atrophy in hindlimb-unweighted rats

Heat stress attenuates skeletal muscle atrophy in hindlimb-unweighted rats

Getting the jump on skeletal muscle disuse atrophy: preservation of contractile performance in aestivatingCyclorana alboguttata(Günther 1867)

Skeletal muscle atrophy occurs slowly and selectively during prolonged aestivation inCyclorana alboguttata(Günther 1867)

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