Oxidative Stress Components Explored in Anoxic and Hypoxic Global Gene Expression Data

Blokhina, Olga; Törönen, Petri; Fagerstedt, Kurt

doi:10.1007/978-3-7091-1254-0_2

Cited by 10 publications

(11 citation statements)

References 105 publications

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“…As observed in Arabidopsis (Blokhina et al, 2014 ), one of the main effects of hypoxic conditions in apple cortex is modulation of the oxygenase expression. In addition, the differential expression of a large number of members of the superfamily of cytochromes P450 may indicate the presence of finely-tuned mechanisms activating oxygen in signaling events (Lewis, 2002 ).…”

Section: Discussionmentioning

confidence: 76%

Extreme Hypoxic Conditions Induce Selective Molecular Responses and Metabolic Reset in Detached Apple Fruit

et al. 2016

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The ripening physiology of detached fruit is altered by low oxygen conditions with profound effects on quality parameters. To study hypoxia-related processes and regulatory mechanisms, apple (Malus domestica, cv Granny Smith) fruit, harvested at commercial ripening, were kept at 1°C under normoxic (control) and hypoxic (0.4 and 0.8 kPa oxygen) conditions for up to 60 days. NMR analyses of cortex tissue identified eight metabolites showing significantly different accumulations between samples, with ethanol and alanine displaying the most pronounced difference between hypoxic and normoxic treatments. A rapid up-regulation of alcohol dehydrogenase and pyruvate-related metabolism (lactate dehydrogenase, pyruvate decarboxylase, alanine aminotransferase) gene expression was detected under both hypoxic conditions with a more pronounced effect induced by the lowest (0.4 kPa) oxygen concentration. Both hypoxic conditions negatively affected ACC synthase and ACC oxidase transcript accumulation. Analysis of RNA-seq data of samples collected after 24 days of hypoxic treatment identified more than 1000 genes differentially expressed when comparing 0.4 vs. 0.8 kPa oxygen concentration samples. Genes involved in cell-wall, minor and major CHO, amino acid and secondary metabolisms, fermentation and glycolysis as well as genes involved in transport, defense responses, and oxidation-reduction appeared to be selectively affected by treatments. The lowest oxygen concentration induced a higher expression of transcription factors belonging to AUX/IAA, WRKY, HB, Zinc-finger families, while MADS box family genes were more expressed when apples were kept under 0.8 kPa oxygen. Out of the eight group VII ERF members present in apple genome, two genes showed a rapid up-regulation under hypoxia, and western blot analysis showed that apple MdRAP2.12 proteins were differentially accumulated in normoxic and hypoxic samples, with the highest level reached under 0.4 kPa oxygen. These data suggest that ripe apple tissues finely and specifically modulate sensing and regulatory mechanisms in response to different hypoxic stress conditions.

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Section: Discussionmentioning

confidence: 76%

Extreme Hypoxic Conditions Induce Selective Molecular Responses and Metabolic Reset in Detached Apple Fruit

et al. 2016

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“…Studies in an array of species and O 2 conditions have noted elevation of transcripts associated with response to oxidative stress, emphasizing their importance (Branco‐Price et al ., ; Blokhina et al ., ). There are also many demonstrated instances of ROS production or damage in response to reoxygenation (reviewed by Fukao & Bailey‐Serres, ; Bailey‐Serres & Chang, ; Blokhina & Fagerstedt, ).…”

Section: After the Delugementioning

confidence: 97%

Flood adaptive traits and processes: an overview

2015

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Summary Unanticipated flooding challenges plant growth and fitness in natural and agricultural ecosystems. Here we describe mechanisms of developmental plasticity and metabolic modulation that underpin adaptive traits and acclimation responses to waterlogging of root systems and submergence of aerial tissues. This includes insights into processes that enhance ventilation of submerged organs. At the intersection between metabolism and growth, submergence survival strategies have evolved involving an ethylene‐driven and gibberellin‐enhanced module that regulates growth of submerged organs. Opposing regulation of this pathway is facilitated by a subgroup of ethylene‐response transcription factors (ERFs), which include members that require low O2 or low nitric oxide (NO) conditions for their stabilization. These transcription factors control genes encoding enzymes required for anaerobic metabolism as well as proteins that fine‐tune their function in transcription and turnover. Other mechanisms that control metabolism and growth at seed, seedling and mature stages under flooding conditions are reviewed, as well as findings demonstrating that true endurance of submergence includes an ability to restore growth following the deluge. Finally, we highlight molecular insights obtained from natural variation of domesticated and wild species that occupy different hydrological niches, emphasizing the value of understanding natural flooding survival strategies in efforts to stabilize crop yields in flood‐prone environments.

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“…), resulting in transcriptional reprogramming of a wide‐array of genes, most notably those involved in metabolism and the stress response (Blokhina et al . ). Moreover, the dimerized protein complex of HIF‐1 is crucial in the cellular shift from oxidative phosphorylation to glycolysis, as demonstrated by the concurrent increase of the glucose import proteins GLUT‐1 and GLUT‐4 (Clerici & Matthay, ), and the upregulation of its transcriptional target, pyruvate dehydrogenase kinase (PDK), which blocks the conversion of pyruvate to acetyl CoA (the first substrate of the mitochondrial TCA cycle) by pyruvate dehydrogenase.…”

Section: Introductionmentioning

confidence: 97%

The role of declining adaptive homeostasis in ageing

Pomatto

Davies

2017

The Journal of Physiology

156

108

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Adaptive homeostasis is "the transient expansion or contraction of the homeostatic range for any given physiological parameter in response to exposure to sub-toxic, non-damaging, signalling molecules or events, or the removal or cessation of such molecules or events" (Davies, 2016). Adaptive homeostasis enables biological systems to make continuous short-term adjustments for optimal functioning despite ever-changing internal and external environments. Initiation of adaptation in response to an appropriate signal allows organisms to successfully cope with much greater, normally toxic, stresses. These short-term responses are initiated following effective signals, including hypoxia, cold shock, heat shock, oxidative stress, exercise-induced adaptation, caloric restriction, osmotic stress, mechanical stress, immune response, and even emotional stress. There is now substantial literature detailing a decline in adaptive homeostasis that, unfortunately, appears to manifest with ageing, especially in the last third of the lifespan. In this review, we present the hypothesis that one hallmark of the ageing process is a significant decline in adaptive homeostasis capacity. We discuss the mechanistic importance of diminished capacity for short-term (reversible) adaptive responses (both biochemical and signal transduction/gene expression-based) to changing internal and external conditions, for short-term survival and for lifespan and healthspan. Studies of cultured mammalian cells, worms, flies, rodents, simians, apes, and even humans, all indicate declining adaptive homeostasis as a potential contributor to age-dependent senescence, increased risk of disease, and even mortality. Emerging work points to Nrf2-Keap1 signal transduction pathway inhibitors, including Bach1 and c-Myc, both of whose tissue concentrations increase with age, as possible major causes for age-dependent loss of adaptive homeostasis.

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Oxidative Stress Components Explored in Anoxic and Hypoxic Global Gene Expression Data

Cited by 10 publications

References 105 publications

Extreme Hypoxic Conditions Induce Selective Molecular Responses and Metabolic Reset in Detached Apple Fruit

Extreme Hypoxic Conditions Induce Selective Molecular Responses and Metabolic Reset in Detached Apple Fruit

Flood adaptive traits and processes: an overview

The role of declining adaptive homeostasis in ageing

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