Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles

Huang, Chuan-Chin; Aronstam, Robert S.; Chen, Da‐Ren; Huang, Yue-Wern

doi:10.1016/j.tiv.2009.09.007

Cited by 371 publications

(235 citation statements)

References 39 publications

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“…In wild-type, the presence of metalloproteins provides antioxidant protection to the organism; this protection is significantly impaired in the triple knockout mutant, findings that mirror those of others (Sharma et al, 2012a,b;Ahamed et al, 2011;Sharma et al, 2009;Hanley et al, 2009;Huang et al, 2010).…”

Section: Resultssupporting

confidence: 72%

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

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Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Spurgeon, David J.; Sturzenbaum, Stephen R.. 2014. Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegansContact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. suggesting that the nominal exposure to pure ZnONPs represents in vivo, at best, a mixture exposure of ionic zinc and nanoparticles. Nevertheless, the analyzes provided evidence that the metallothioneins (mtl-1 and mtl-2), the pytochelatin synthase (pcs-1) and an apoptotic marker (cep-1) were transcriptionally activated. In addition, the DCFH-DA assay provided in vitro evidence of the oxidative potential of ZnONPs in the metal exposure sensitive triple mutant.Raman spectroscopy highlighted that the biomolecular phenotype changes significantly in the metal sensitive knockout worm upon ZnONP exposure, suggesting that these metalloproteins are instrumental in the protection against cytotoxic damage. Finally, ZnONP exposure was shown to decrease growth and development, reproductive capacity and lifespan, effects which were amplified in the triple knockout. By combining diverse toxicological strategies, we identified that individuals (genotypes) housing mutations in key metalloproteins are more susceptible to ZnONP exposure, which underlines the importance of fully functional metalloproteins to minimize ZnONP induced toxicity.

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Section: Resultssupporting

confidence: 72%

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Polak

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Jurkschat

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Results also demonstrated DNA damage, oxidative stress and mitochondrial damage [218]. Similarly, when human bronchial epithelial cells were treated with zinc oxide at 100 μg/mL, the observed effects include the release of LDH, decrease in cell viability and oxidative stress [219]. The toxicity of titanium nanoparticles affects differentiation, cell proliferation, apoptosis and mobility [220,221].…”

Section: Mechanistic Studiesmentioning

confidence: 93%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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“…Notably, its selective cytotoxic effect on cancer cells is among its important features in this regard [112,113]. NPs induced ROS generation and consequent OS is frequently observed and is the most discussed paradigm for NP toxicity [114,115]. Cytotoxic potential of ZnO is attributed to many features, including induction of oxidative stress through generation of ROS on the surface of particles [115][116][117], dissolution and release of Zn2+ ions and physical interaction of ZnO NPs with the membrane wall cause deformation and rupture of membrane [118].…”

Section: Camel's Milk and Urine Nanoparticlesmentioning

confidence: 99%

“…Accordingly, numerous of camel urine compounds have been also used for such applications. Notably, multifunctional zinc oxide (ZnO) is used in various forms such as NPs or nanorods for biomedical applications including biosensing, imaging, drug delivery, and clinical implants [113,114,146] the additive or synergistic effects of ZnO NPs with anti-cancer compounds (or drugs) was found to upregulate induction of apoptosis in cancer cells [147]. Hippuric acid nanocomposite (HAN) (a preparation from hippuric acid (HA) and ZnO) combination with doxorubicin and/or with oxaliplatin induce suppression of cell proliferation and exhibit supreme cytotoxicity in several cancer cell lines as compared to individual effects.…”

Section: Development Efforts On Camel Products Based Anticancer Nanopmentioning

confidence: 99%

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

Alebie¹,

Yohannes²,

Worku³

2017

J Cancer Sci Ther

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Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles

Cited by 371 publications

References 39 publications

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

Metalloproteins and phytochelatin synthase may confer protection against zinc oxide nanoparticle induced toxicity in Caenorhabditis elegans

In vitro and in vivo toxicity assessment of nanoparticles

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

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