Oxidative Stress Biomarkers in the Muscle Tissue of the Rainbow Trout (ONCORHYNCHUS MYKISS WALBAUM) After In Vitro Treatment of Sansevieria Caulescens N.E.Br. Extract

Abstract

The goal of this study was to assess in vitro the effect of buffer extract obtained from leaves of Sansevieria caulescens N.E.Br. on the 2-thiobarbituric acid reactive substances (TBARS) as lipid peroxidation biomarker, aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity (TAC) in the muscle tissue of the rainbow trout. Our study suggests that the leaf S. caulescens extract have shown good antioxidant potential in vitro study after incubation with muscle tissue homogenate of rainbow trout. There were no significant changes for TBARS level as biomarker of lipid peroxidation, aldehydic and ketonic derivatives of oxidatively modified proteins between values in control group and in the muscle tissue of rainbow trout after incubation with extracts from leaves of S. caulescens. Our results showed that extract of S. caulescens efficiently increased the total antioxidant capacity in muscle tissue by 46.6% (p <0.05) due to inhibited the Fe 2+ /ascorbate-induced oxidation of Tween 80, resulting in a decrease in the TBARS level. Taking into account existing experimental evidence, it is reasonable to assume that secondary plant metabolites, i.e. polyphenolic compounds in the extract of S. caulescens may contribute to the antioxidant activity. Further studies including both the use of other medicinal plants as food additives in aquaculture and the assessment of its antioxidant effects on various tissues are in progress.

Lipid Peroxidation and Total Antioxidant Capacity in the Muscle Tissue of Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus Mitchill) after In Vitro Treatment by Extracts Derived from Various Species of Dracaena Genus (Asparagaceae Juss.)

“…Although antimicrobial and antioxidant activities of extracts derived from leaves of various species belonging to the Dracaena genus were investigated (Al-Fatimi et al, 2007;Buyun et al, 2016Buyun et al, , 2017Maryniuk et al, 2017Maryniuk et al, , 2018Maryniuk et al, , 2019Tkachenko et al, 2017aTkachenko et al, , 2017bTkachenko et al, , 2018Tkachenko et al, , 2019aTkachenko et al, , 2019b, studies regarding their protective effects against free radical-induced oxidative stress in the muscle tissue of Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus Mitchill) have not yet been undertaken. Therefore, the aim of the current study was to evaluate in vitro the effect of buffer extracts derived from leaves of selected species belonging to the Dracaena genus (in final concentration 10 mg.mL -1 ) against lipid peroxidation and protein damage using 2-thiobarbituric acid reactive substances and total antioxidant capacity.…”