Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees

Kojić, Danijela; Purać, Jelena; Nikolić, Tatjana; Orčić, Snežana; Vujanović, Dragana; Ilijević, Konstantin; Vukašinović, Elvira L.; Blagojević, Duško

doi:10.1127/entomologia/2019/0743

Cited by 10 publications

(8 citation statements)

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“…ROS (reactive oxygen species) are inevitably involved in the biology of honey bees. For example, they are the honey bees' effective weapons against pathogens [35], but are prone to variations [36]. Their levels were found to be lower in wintering bees, which may imply the reason for higher susceptibility to diseases [35].…”

Section: Discussionmentioning

confidence: 99%

“…However, it has been known that some parameters of oxidative stress, (CAT and GST activities, and MDA concentrations) may be infl uenced by numerous factors, such as living in greenhouses [54] or industrial areas [27], migratory beekeeping [55], seasonality [35,36], fl ying activities [36,56], aging [36,56] and brood rearing [36]. Although radio frequency electromagnetic fi eld of certain strength can infl uence oxidative stress, it is not likely to happen in realistic conditions [57].…”

Section: Discussionmentioning

confidence: 99%

“…Statistical analyses. The data obtained were processed with GraphPad Prism (GraphPad Software), as described by Kojic et al [36]. Owing to the homogeneity of the data (cv<30%), the groups were compared in a two-way repeated measures ANOVA, which was followed by Tukey's or Sidak's multiple comparisons test.…”

Section: Methodsmentioning

confidence: 99%

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Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

et al. 2020

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Apart from the efficiency of coumaphos against Varroa mites, its impact on the oxidative status and survival of the honey bee (Apis mellifera) was assessed. The research was conducted on hives from the same apiary, equalised regarding the number of bees, brood area and food storage. Based on Varroa infestation the hives were allotted to two groups: non-infested (N) and infested (I). Both groups were either treated (T) – NT and IT, or untreated (U) – NU and IU. The treatment of infested bees was controlled with a follow-up treatment with amitraz. The efficiency of coumaphos was 96-97%. This organophosphate had a negligible effect on bee survival, but it significantly affected their oxidative status: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and the concentrations of malonyl dialdehyde (MDA). Coumaphos significantly (p˂0.0001) decreased SOD activity in non-infested bees, but increased it in those infested. By contrast, both CAT and GST activities, as well as MDA concentrations significantly increased (from p˂0.05 to p˂0.0001) after treatment in all groups, with the exception of IT, where it declined. Coumaphos in non-infested hives caused oxidative stress per se, not unlike varroa in infested colonies. However, in infested colonies it decreased oxidative stress, owing to its efficacy against Varroa mites and contributed to the recovery of bee colonies. In spite of its certain downsides, coumaphos remains an effective anti-varroa substance, but should be used with precaution, not to add to the effects of environmental factors which may cause red-ox misbalance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

et al. 2020

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“…This not only preserves limited nutrient stores but also provides a break in the larval disease cycle and suppresses the Varroa mite, the major parasite of developing brood. While immunity and antioxidant expression increases from early to late winter [ 13 ], differences in immunity profiles between summer and winter bees suggests evolutionary adaptations to both conserve energy and resist bacterial pathogens overwinter [ 8 , 14 ]. Consistent with these evolutionary adaptations, beekeepers have found success overwintering honey bee colonies indoors under controlled cold climate conditions of 7 °C and 25% relative humidity [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Overwintering Honey Bee Colonies: Effect of Worker Age and Climate on the Hindgut Microbiota

Maes

Floyd

Mott

et al. 2021

Insects

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Honey bee overwintering health is essential to meet the demands of spring pollination. Managed honey bee colonies are overwintered in a variety of climates, and increasing rates of winter colony loss have prompted investigations into overwintering management, including indoor climate controlled overwintering. Central to colony health, the worker hindgut gut microbiota has been largely ignored in this context. We sequenced the hindgut microbiota of overwintering workers from both a warm southern climate and controlled indoor cold climate. Congruently, we sampled a cohort of known chronological age to estimate worker longevity in southern climates, and assess age-associated changes in the core hindgut microbiota. We found that worker longevity over winter in southern climates was much lower than that recorded for northern climates. Workers showed decreased bacterial and fungal load with age, but the relative structure of the core hindgut microbiome remained stable. Compared to cold indoor wintering, collective microbiota changes in the southern outdoor climate suggest compromised host physiology. Fungal abundance increased by two orders of magnitude in southern climate hindguts and was positively correlated with non-core, likely opportunistic bacteria. Our results contribute to understanding overwintering honey bee biology and microbial ecology and provide insight into overwintering strategies.

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“…Uncontrolled production of ROS impairs the antioxidant system, causingincreased oxidative stress, which affects spermatozoa activity, damages DNA structure, and accelerates apoptosis, sperm motility, viability, and numbers(22–24). Thus, the evolution of a strong anti-oxidative system comprising enzymes such as heme peroxidase (HPX), Duox, catalase, SOD, GST, and other non-enzymatic factors, is believed to play a key role in maintaining optimal homeostasis for long-term storage and survival of sperms (25,26). But the mechanism of this regulation remains unknown in mosquitoes until a recent demonstration that HPX15 activation ensures not only survival of Plasmodium in the gut(27)alsohealthy sperm in the spermathecal of mated adult female An.…”

Section: Introductionmentioning

confidence: 99%

A testis-specific Heme Peroxidase HPX12 regulates male fertility in the mosquitoAnopheles stephensi

Kumari

Chauhan

Rani

et al. 2020

Preprint

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AbstractIn humans, dysregulation of the antioxidant defense system has a detrimental impact on male fertility and reproductive physiology. Establishing such a correlation in disease vectors may pave a new way to manipulate male’s reproductive physiology, but remains the least attended. Since long - term storage of healthy and viable sperm earmarks male’s reproductive competency, we tested whether the anti-oxidative system protein also influences male fertility in the mosquito An. stephensi. We showed that a testis-specific HPX12 is critical for maintaining the physiological homeostasis of male mosquito’s reproductive organs. Disruption of this antioxidant enzyme by dsRNA silencing in the male mosquito severely impairs the reproductive potential of mated blood-fed female mosquitoes,resulting in a loss of 60% eggs. Our data demonstrate that increased ROS in the HPX12 mRNA depleted mosquitoes is an ultimate cause of sperm disabilities both qualitatively as well as quantitatively.

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Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees

Cited by 10 publications

References 0 publications

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees

Overwintering Honey Bee Colonies: Effect of Worker Age and Climate on the Hindgut Microbiota

A testis-specific Heme Peroxidase HPX12 regulates male fertility in the mosquitoAnopheles stephensi

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