Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess

Ranieri, Annamaria; Castagna, Antonella; Scebba, Francesca; Careri, Maria; Zagnoni, Ingrid; Predieri, Giovanni; Pagliari, M.; Toppi, Luigi Sanità di

doi:10.1016/j.plaphy.2004.12.004

Cited by 114 publications

(48 citation statements)

References 44 publications

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“…The synthesis of PCs in response to Cd has been reported in a number of studies conducted on various species including a marine diatom (Thalassiosira nordenskioeldii, Wang and Wang, 2011), freshwater green alga (Scenedesmus vacuolatus, Le Faucheur et al, 2005), tobacco cell culture (Nicotiana tabacum, Zitka et al, 2011), bread wheat (Triticum aestivum, Ranieri et al, 2005), rice (Nocito et al, 2011) and broad bean (Vicia faba, Čabala et al, 2011). The fact that no PCs were detected in the roots of lettuce grown in either Cd treatment indicates that PCs were not involved in Cd 2+ chelation and accumulation in lettuce root.…”

Section: Phytochelatinsmentioning

confidence: 96%

“…It has been suggested that GSH might act as a first line of defense against Cd 2+ toxicity by complexing metal ions before sufficient PCs are synthesized (Thangavel et al, 2007). Once PCs take over the detoxification process, GSH gets involved in a secondary defense mechanism by scavenging free radicals in Cd 2+ -induced oxidative stress (Gallego et al, 2005;Ranieri et al, 2005). Thus, GSH is not only a precursor for PC synthesis (Grill et al, 1989) but also an important antioxidant in plants.…”

Section: Precursor Peptidesmentioning

confidence: 99%

“…Previous studies have reported Cd-induced PC synthesis (Grill et al, 1985;Ranieri et al, 2005;Wang and Wang, 2011) and identified Cd 2+ -PC n complexes either under laboratory conditions (Kobayashi and Yoshimura, 2006;Chekmeneva et al, 2007; or from plants in their native environment (Scarano and Morelli, 2002;Chen et al, 2007) as evidence for the proposed mechanism (Sanità di Toppi and Gabbrielli, 1999) involved in Cd detoxification in plants.…”

Section: Introductionmentioning

confidence: 98%

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Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

Akhter

McGarvey

Macfie

2012

Journal of Plant Physiology

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SummaryCadmium (Cd) is a non-essential trace element and its environmental concentrations are approaching toxic levels, especially in some agricultural soils. Understanding how and where Cd is stored in plants is important for ensuring food safety. In this study, we examined two plant species that differ in the distribution of Cd among roots and leaves. Lettuce and barley were grown in nutrient solution under two conditions: chronic (4 week) exposure to a low, environmentally relevant concentration (1.0 µM) of Cd and acute (1 h) exposure to a high concentration (5.0 mM) of Cd. Seedlings grown in solution containing 1.0 µM CdCl 2 did not show symptoms of toxicity and, at this concentration, 77% of the total Cd was translocated to leaves of lettuce, whereas only 24% of the total Cd was translocated to barley leaves. We tested the hypothesis that differential accumulation of Cd in roots and leaves is related to differential concentrations of phytochelatins (PCs), and its precursor peptides. The amounts of PCs and their precursor peptides in the roots and shoots were measured using HPLC. Each of PC 2-4 was synthesized in the barley root upon chronic exposure to Cd and did not increase further upon acute exposure. In the case of lettuce, no PCs were detected in the root given either Cd treatment.The high amounts of PCs produced in barley root could have contributed to preferential retention of Cd in barley roots.

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Section: Phytochelatinsmentioning

confidence: 96%

Section: Precursor Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

Akhter

McGarvey

Macfie

2012

Journal of Plant Physiology

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“…In spite of that Cd is a non-redox-active metal; its toxicity is undoubtedly connected with increased levels of ROS. An enhanced level of lipid and protein peroxidation, increased level of ROS and activation of antioxidant apparatus indicated that in HM-stressed roots the presence of oxidative stress is a general syndrome in several plant species (Hegedüs et al 2001;Schützendübel et al 2001;Ranieri et al 2005;Garnier et al 2006;Rellán-Á lvarez et al 2006;Rodríguez-Serrano et al 2006;Ortega-Villasante et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Role of reactive oxygen species-generating enzymes and hydrogen peroxide during cadmium, mercury and osmotic stresses in barley root tip

Tamás

Mistrı́k

Huttová

et al. 2009

Planta

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The effect of cadmium (Cd) on the expression and activity of NADPH oxidase, peroxidase and oxalate oxidase as well as on the expression of aquaporins and dehydrins was studied in barley root tip. The root tip represented intact apical part of the barley root containing the root cap, meristems and elongation zone. Except stress induced by Cd, barley root tips were analysed after their exposure to phytotoxic concentration of mercury (Hg)-, hydrogen peroxide (H2O2)- or polyethylene glycol (PEG)-induced water stress in order to compare the Cd-induced changes with changes induced by these other stress factors. Cd, Hg, H2O2 and with some exceptions also PEG treatments caused similar alterations in the gene expression of reactive oxygen species (ROS)-generating and water deficiency-related genes, and in the activity of ROS-generating enzymes. These evidences support our opinion that ROS accumulation and water imbalance are the common symptoms of these stress factors and that the elevated production of H2O2 plays, probably as a signal molecule, a key role in the induction of plant responses to abiotic stresses in barley root tip. On the other hand, H2O2 at permanent high concentration is probably the main toxic factor during stress conditions.

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“…Plants synthesize metal-chelating peptides, such as GSH and phytochelatins, or Cys-rich proteins. such as metallothioneins, that bind to Cd(II) and reduce its toxicity (9,10).…”

mentioning

confidence: 99%

Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast

Kim

Shim

et al. 2008

Journal of Biological Chemistry

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Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess

Cited by 114 publications

References 44 publications

Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

Role of reactive oxygen species-generating enzymes and hydrogen peroxide during cadmium, mercury and osmotic stresses in barley root tip

Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast

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