Oxidative Stress and Its Association with Male Infertility

Agarwal, Ashok; Sengupta, Pallav

doi:10.1007/978-3-030-32300-4_6

Cited by 47 publications

(42 citation statements)

References 98 publications

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“…1); this finding agrees with previously reported results [24,25]. The negative correlation between ROS and antioxidants has been confirmed; low seminal antioxidant potential allows ROS overproduction in infertile men [26]. The exposure to ROS is extremely destructive to sperm plasma membrane [12].…”

Section: Discussionsupporting

confidence: 90%

Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

Palani

Alahmar

2020

Afr J Urol

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Background Oxidative stress has been implicated in male infertility through decrease in sperm quality. However, men with normal semen parameters (normozoospermia) may be unable to fertilize their partners even when they have normal sperm function. Thus, they would be considered infertile. The purpose of this study was to investigate the role of oxidative stress in the pathogenesis of unexplained male infertility. Methods In this case–control study, infertile men with normozoospermia (n = 46) and fertile control group (n = 21) underwent seminal fluid analyses according to WHO 2010 criteria. Serum and seminal plasma levels of total antioxidant capacity (TAC), glutathione, malondialdehyde, uric acid and albumin were also measured using colorimetric methods. Results The level of total antioxidant capacity in both serum and seminal plasma was significantly lower in normozoospermic infertile men in comparison with fertile group (p < 0.0001). However, no significant differences were observed in serum and seminal plasma levels of glutathione, uric acid, albumin and malondialdehyde between infertile and fertile groups. Conclusion Low TAC level induces oxidative stress and consequently causes sperm dysfunction and male infertility. Estimation of TAC can be a useful tool in the diagnosis of male infertility. Antioxidant supplementation should be considered in the treatment of oxidative stress-induced male infertility.

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Section: Discussionsupporting

confidence: 90%

Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

Palani

Alahmar

2020

Afr J Urol

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“…ROS have a determinant role regarding the causes of abnormal sperm function. Excessive ROS production the seminal plasma seems to be associated with reduced sperm fertilising potential, impaired metabolism, motility and morphology (Agarwal & Sengupta, 2020). ROS‐induced cell damage occurs due to an unbalanced cell redox, especially when the antioxidant systems cannot compensate for the increased ROS.…”

Section: Discussionmentioning

confidence: 99%

“…Depending on the concentration, location and time of exposure, ROS can have a beneficial or harmful effect on spermatozoa. At normal physiological levels, ROS are essential for motility, capacitation, hyperactivation, acrosome reaction and, therefore, fertilisation (Agarwal & Sengupta, 2020; Di Meo et al., 2016). Overproduction of free radicals exceeding the antioxidant capacity of both the spermatozoa and the seminal plasma, known as oxidative stress (OS), can lead to apoptosis, lipid peroxidation, low sperm quality, and protein and DNA damage (Aitken, 2017; Subramanian et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA damage and seminal antioxidant activity in subfertile men

et al. 2021

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Supraphysiological ROS levels can lead to apoptosis, lipid peroxidation, and DNA and protein damage. This pilot study aimed to investigate the sperm oxidative damage in subfertile men, to describe the relationship between the antioxidant system and ROS. Sixty‐four semen samples were categorised according to the evaluated routine parameters (WHO, WHO laboratory manual for the examination and processing of human semen, 2010). Results were cross‐referenced with the DNA damage [Comet (n = 53) and TUNEL (n = 49) assays], antioxidant enzyme activity [SOD (n = 51), CAT (n = 48) and GST (n = 48)], and content of total thiols (n = 36), lipid hydroperoxides (n = 35) and MDA (n = 31). Compared to pathospermic samples, normozoospermic presented 40%–45% fewer spermatozoa with fragmented DNA, 19% fewer hydroperoxides, and slightly higher total thiols and MDA levels. Asthenozoospermic/asthenoteratozoospermic samples had the lowest GST activity. SOD and CAT showed a similar trend. Our results evidenced significant positive correlations between DNA damage and immotile spermatozoa; SOD and CAT, GST and total thiols; CAT and GST; total thiols and sperm concentration; and MDA levels and head/midpiece abnormalities and hydroperoxides. This work contributes to the existing body of knowledge by showing that the oxidative status correlates with the classic sperm analysis parameters. Oxidative stress and DNA damage evaluation might be a valuable diagnostic and prognostic tool in cases of idiopathic male subfertility.

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“…ROS are major causes of sperm DNA damage (Agarwal & Sengupta, 2020). ROS affect spermatozoa mainly during the transit through the epididymis where they enhance endonuclease and caspases activities, resulting in an increasing rate of germ cell apoptosis (Sakkas & Alvarez, 2010).…”

Section: What Are the Current Guidelines For Testing Sperm Dna Intementioning

confidence: 99%

Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation

2020

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Male infertility has a complex etiology, and many times, the cause is unknown. While routine semen analysis provides an overview of basic semen parameters, such as sperm concentration, motility, viability and morphology, a significant overlap of these parameters has been reported in fertile and infertile men. Moreover, conventional semen parameters do not reveal the cellular or molecular mechanisms of sperm dysfunctions leading to infertility. Therefore, sperm functional parameters, including sperm chromatin integrity, are evaluated to provide information on subtle sperm defects that are not routinely identified. Incomplete or defective sperm chromatin condensation increases the susceptibility of the sperm DNA to oxidative damage or other factors. To evaluate sperm chromatin integrity, different methods with varying degrees of diagnostic and prognostic capabilities are available. Among these assays, SCSA, TUNEL and SCD assays are most commonly used. While these assays rather evaluate the DNA directly for damages, the aniline blue and chromomycin A3 stains test for the quality of chromatin condensation. Thus, this review discusses and compares different methods used to evaluate sperm chromatin integrity and condensation, and their inclusion in the routine evaluation of the male infertility.

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Oxidative Stress and Its Association with Male Infertility

Cited by 47 publications

References 98 publications

Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

Sperm DNA damage and seminal antioxidant activity in subfertile men

Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation

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