Oxidative stress and endoreduplication induced by blue light exposure to CHO cells

Wall, Alexa C.; Gius, Jeffrey P.; Buglewicz, Dylan J.; Banks, Austin B.; Kato, Takamitsu A.

doi:10.1016/j.mrgentox.2019.05.003

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…cell death in gingival fibroblasts 4 and lens epithelial fibroblasts, 5 to generate sister-chromatid exchanges and endoreduplicated chromosomes in CHO cells, 6 and to cause deoxyribonucleic acid (DNA) strand breaks in retinal pigment epithelial cells. 7 Blue wavelengths near ultra-violet A (UVA) (400-450 nm), shown to be the most energetic visible wavelengths, have been supposed to produce the most important adverse effects in mammalian cells, 8 mainly through the extracellular and intracellular photosensitized production of reactive oxygen species (ROS).…”

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confidence: 99%

Blue light induces DNA damage in normal human skin keratinocytes

Chamayou-Robert

Digiorgio

Brack

et al. 2021

Photoderm Photoimm Photomed

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Blue light (400-500 nm) takes part of the visible electromagnetic spectrum. Natural sunlight blue wavelengths, which are always mixed with green, yellow, and red wavelengths, have been shown to play an important role in the regulation of sleeping patterns and circadian rhythms. 1 However, since the 1980s the development of lightemitting diodes (LED) without red and near-infrared wavelengths and the concomitant exponential use of digital technologies, such as flat-screen TVs, computers, tablets, and smartphones, have led to a drastic increase in human exposure to blue light 2 and to several concerns about their possible involvement in the development of degenerative eye diseases. Moreover, the expansion of therapeutic protocols using blue light irradiation in several dental treatments and dermatologic therapies has highlighted their potential side effects. 3 The toxic and genotoxic effects of blue wavelengths have mainly been studied in the context of their therapeutic and ocular risks: They have been shown to induce cellular dysfunction and

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confidence: 99%

Blue light induces DNA damage in normal human skin keratinocytes

Chamayou-Robert

Digiorgio

Brack

et al. 2021

Photoderm Photoimm Photomed

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“…Images were captured by a Diagnostic Instrument SPOT camera and software. Overall, 1000 metaphase cells were analyzed for endoreduplication formation analysis [ 30 , 45 ]. Three independent experiments were performed.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the Genotoxic and Cytotoxic Effects of Thymidine Analogs, 5-Ethynyl-2′-Deoxyuridine and 5-Bromo-2′-Deoxyurdine to Mammalian Cells

Haskins

Maeda

et al. 2020

IJMS

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BrdU (bromodeoxyuridine) and EdU (ethynyldeoxyuridine) have been largely utilized as the means of monitoring DNA replication and cellular division. Although BrdU induces gene and chromosomal mutations and induces sensitization to photons, EdU‘s effects have not been extensively studied yet. Therefore, we investigated EdU’s potential cytotoxic and mutagenic effects and its related underlying mechanisms when administered to Chinese hamster ovary (CHO) wild type and DNA repair-deficient cells. EdU treatment displayed a higher cytotoxicity and genotoxicity than BrdU treatment. Cells with defective homologous recombination repair displayed a greater growth delay and severe inhibition of clonogenicity with EdU compared to wild type and other DNA repair-deficient cells. Inductions of sister chromatid exchange and hypoxanthine phosphorybosyl transferase (HPRT) mutation were observed in EdU-incorporated cells as well. Interestingly, on the other hand, EdU did not induce sensitization to photons to the same degree as BrdU. Our results demonstrate that elevated concentrations (similar to manufacturers suggested concentration; >5–10 μM) of EdU treatment were toxic to the cell cultures, particularly in cells with a defect in homologous recombination repair. Therefore, EdU should be administered with additional precautions.

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“…51 The light of the blue range of the visible spectrum is less harmful but is still able to damage both photosensitive retinal cells and normal embryonic cells, causing oxidative stress. 52,53 Therefore, weak light power of 5, 10 and 20 mJ s −1 with total doses of 1, 2, 3 and 5 J cm −2 was chosen to study the phototoxicity of blue 420 nm light on LNCaP prostate cancer cells. A wavelength of 420 nm was used for irradiation because it coincides with the Soret absorption peak of the ZnTPP molecule.…”

Section: Cytotoxicity Of Nanohybrids To Cancer Cellsmentioning

confidence: 99%