Oxidative stress and electron spin resonance

Kopáni, Martin; Celec, Peter; Danišovič, Ľuboš; Michalka, Peter; Bíró, Csaba

doi:10.1016/j.cca.2005.05.016

Cited by 87 publications

(68 citation statements)

References 85 publications

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“…The most widely recognized spin traps are alphaphenyl N-tertiary-butyl nitrone (PBN) and 5,5-dimethylpyrroline N-oxide. Although spin traps are commonly used for identifying free radical species via electron spin resonance, they also serve a biological role as stabilizers of ROS generated in cells undergoing oxidative stress [66,67]. For example, intravenous PBN treatment has been shown to improve energy metabolism in the injured spinal cord, possibly through the reduction of free radical-mediated mitochondrial dysfunction [68].…”

Section: Figmentioning

confidence: 99%

Targeting Mitochondrial Function for the Treatment of Acute Spinal Cord Injury

et al. 2011

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Summary: Traumatic injury to the mammalian spinal cord is a highly dynamic process characterized by a complex pattern of pervasive and destructive biochemical and pathophysiological events that limit the potential for functional recovery. Currently, there are no effective therapies for the treatment of spinal cord injury (SCI) and this is due, in part, to the widespread impact of the secondary injury cascades, including edema, ischemia, excitotoxicity, inflammation, oxidative damage, and activation of necrotic and apoptotic cell death signaling events. In addition, many of the signaling pathways associated with these cascades intersect and initiate other secondary injury events. Therefore, it can be argued that therapeutic strategies targeting a specific biochemical cascade may not provide the best approach for promoting functional recovery. A "systems approach" at the subcellular level may provide a better strategy for promoting cell survival and function and, as a consequence, improve functional outcomes following SCI. One such approach is to study the impact of SCI on the biology and function of mitochondria, which serve a major role in cellular bioenergetics, function, and survival. In this review, we will briefly describe the importance and unique properties of mitochondria in the spinal cord, and what is known about the response of mitochondria to SCI. We will also discuss a number of strategies with the potential to promote mitochondrial function following SCI.

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Section: Figmentioning

confidence: 99%

Targeting Mitochondrial Function for the Treatment of Acute Spinal Cord Injury

et al. 2011

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“…Oxidative stress can be result of dysbalance between the production of free radicals and antioxidant system which plays a role in the pathogenesis of fatal diseases such as cancer and cardiovascular diseases (Kopani et al, 2006). Excessive production of ROS may lead to programmed cell death and may accelerate the process of aging (Chamond et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Antioxidants May Protect Cancer Cells from Apoptosis Signals and Enhance Cell Viability

Akan

Garip²

2013

Asian Pacific Journal of Cancer Prevention

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Quercetin is one of the most abundant dietary flavonoids widely present in many fruits and vegetables. Previous in vitro studies has shown that quercetin acts as an antioxidant and anti-inflammatory agent and it has potent anticarcinogenic properties as an apoptosis inducer. In this study we examined apoptotic effects of quercetin on the K562 erythroleukemia cell line. K562 cells were induced to undergo apoptosis by hydrogen peroxide. Cell viability and apoptosis level were assessed by annexin V and PI staining methods using flow cytometry. Viability of K562 cells was increased by low dose of quercetin (5-100 μM) for 3 hours. High doses of quercetin proved toxic (100-500 μM, 24 hours) and resulted in decrease of K562 cell viability as expected (p<0.01). As to results, 100 µM quercetin was defined as a protective dose. Also, K562 cell apoptosis due to hydrogen peroxide was decreased in a dose dependent manner. As indicated in previous studies, reduction of superoxides by free radical scavengers like quercetin could be beneficial for prevention of cancer but consumption of such flavonoids during cancer treatment may weaken effects of chemotherapeutics and radiotherapy. Especially cancer patients should be carefully considered for traditional phytotherapy during cancer treatment, which can lead to controversial results.

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“…Normally, tissues have defense antioxidant mechanisms that protect them against the toxic effects of free oxygen radicals (Sivonová et al 2004(Sivonová et al , 2006Weis et al 2005;Kopáni et al 2006). Glutathione is considered to be the most important watersoluble antioxidant within cells which is critical to normal cellular physiological processes (Rahman and MacNee 1999).…”

Section: Introductionmentioning

confidence: 99%

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Fattah

He²,

Fa³

et al. 2010

gpb

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Abstract. This study aimed to investigate the possible protective role of curcumin against renal damage caused by administration of cyclosporine A (CsA) in adult male rats. For this purpose, 27 adult male albino rats were used and divided into three equal groups. Group I (control group) and group II (CsA-treated group) received a daily subcutaneous injection of CsA at a dose of 20 mg/kg b.w. Group III (prophylactic group) received a daily oral curcumin at a dose of 15 mg/kg b.w. simultaneously with CsA. After 21 days, all the animals were anaesthetized and the kidneys were rapidly removed and processed to prepare paraffin sections stained with H&E, PAS and Masson's trichrome. In addition, the glutathione S-transferase (GST) enzyme was detected immunohistochemically. The optical density and the area (in %) of positive GST immunoreactions were measured in the cytoplasm of renal tubules and glomeruli and the data were statistically analyzed. Examination of sections from CsA-treated group showed renal tubules with vacuolated cytoplasm and others with darkly stained pyknotic nuclei. Apical brush borders of proximal tubules were undefined and PAS positive granules were noticed in their cytoplasm. The renal corpuscles contained shrunken glomeruli with widening of their Bowman's spaces. Inflammatory cellular infiltrate and increase in the collagen fibers were observed between the renal tubules. In prophylactic group, the structure of renal tubules and corpuscles were preserved except few tubular darkly stained pyknotic nuclei. Numerous blood vessels, few cellular infiltration and thin collagen fibers were observed between the renal tubules. Statistical analysis of morphometric data showed significant increase in the optical density of GST immunoreactivity in the cells of renal tubules and glomeruli of CsAtreated group when compared with the control or prophylactic groups. However, a significant decrease in the area of GST immunoreactivity in sections from prophylactic group was observed when compared with control or CsA-treated groups. In conclusion, protective effect of curcumin against cyclosporine-induced nephrotoxicity in rats was proven based on the study of histological changes and GST immunoexpression. This study supposes the possible therapeutic applications of curcumin in CsA-treated patients.

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Oxidative stress and electron spin resonance

Cited by 87 publications

References 85 publications

Targeting Mitochondrial Function for the Treatment of Acute Spinal Cord Injury

Targeting Mitochondrial Function for the Treatment of Acute Spinal Cord Injury

Antioxidants May Protect Cancer Cells from Apoptosis Signals and Enhance Cell Viability

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

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