Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

Borrego, Silvia; Vázquez, Antonio; Dası́, Francisco; Cerda, C; Iradi, Antonio; Tormos, Carmen; Sánchez, Juan Escámez; Bagán, Leticia; Boix, Javier; Zaragozá, Cristóbal; Camps, Jordi; Sáez, Guillermo T.

doi:10.3390/ijms14023467

Cited by 82 publications

(63 citation statements)

References 70 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total protein content is measured by the Lowry method [33].

8-oxo-dG assay in serum by HPLC-EC

Assay of 8-oxo-dG in serum was performed following the method by Li et al (2013) [20] with slight modification reported by Borrego el at. (2013) [26]. For 8-oxo-dG separation and quantification, a Waters 515 HPLC Pump (model n° 5200ª) with 5 µm Spherisorb ODS2 column (4.6 mm x 250 mm) and electrochemical detectors (HPLC-EC) was used.…”

Section: Methodsmentioning

confidence: 99%

One-year follow-up of clinical, metabolic and oxidative stress profile of morbid obese patients after laparoscopic sleeve gastrectomy. 8-oxo-dG as a clinical marker

et al. 2017

Self Cite

View full text Add to dashboard Cite

Obesity has grown worldwide over the last few decades. In its different degrees, obesity is accompanied by many clinical and biochemical alterations reflecting the pathological condition of various body tissues. Among the mechanisms underlying the pathogenesis of obesity and associated complications, oxidative stress (OS) may be playing an important role. In the present study, we have characterized at systemic level the degree of OS status in a group of morbid obese patients (BMI>40 kg/m2) at basal sate and its modulation during one year after bariatric surgery using the laparoscopic sleeve gastrectomy (LSG) technique. As compared with normal weight subjects matched in age, peripheral blood mononuclear cells (PBMc) of obese patients present a significant reduction of the antioxidant enzyme activities superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) as well as a significant increase of the oxidized/reduced glutathione ratio (GSSG/GSH) in these cells. Lipid peroxidation is significantly increased in the patient group as shown by the increased levels of malondialdehyde (MDA) in PBMc and the amount of F2-Isoprostanes (F2-IsoPs) released in urine. In addition, the DNA damage product 8-oxo-7,8-2′-deoxyguanosine (8-oxo-dG) was also observed to be increased in serum and urine of morbid obese patients as compared with the control group. After LSG, an improvement of their ponderal and metabolic profile was accompanied by a progressive recovery of antioxidant enzyme activities and the decline of oxidative byproducts both in PBMc and biological fluids. The observed changes of urinary 8-oxo-dG levels correlate positively with its serum concentration, the lipid peroxidation products MDA and F2-IsoPs, triglycerides, glucose, insulin, HOMA index and body weight and negatively with the percentage of weight and BMI loss and antioxidant activities. We conclude that the analysis of urinary 8-oxo-dG could be validated as a useful marker for the monitoring of ponderal and metabolic status of morbid obese patients.

show abstract

“…Total protein content is measured by the Lowry method [33].

8-oxo-dG assay in serum by HPLC-EC

Section: Methodsmentioning

confidence: 99%

One-year follow-up of clinical, metabolic and oxidative stress profile of morbid obese patients after laparoscopic sleeve gastrectomy. 8-oxo-dG as a clinical marker

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…6,9,11,14,15,22,34,35,37 Oxidative stress leads to oxidative DNA damage, making 8-oxoguanosine, a marker for oxidative DNA damage, an ideal candidate biomarker for detecting and assessing gastric cancer. 15,47 A defective antioxidant response Heme oxygenase 1 (HO-1), a potent antioxidant and antiinflammatory enzyme that is responsible for the degradation of heme, has well-studied and wide-ranging protective functions across many disease models. 49 HO-1 expression is induced by the presence of nitric oxide (NO), leading to decreased IL-8 expression and inflammation.…”

Section: Increased Expression Of Spermine Oxidase During H Pylori Inmentioning

confidence: 99%

“…15,47 8-oxoguanosine and 8-OHdG assays measure oxodized nucleotide adducts, which form as a result of oxidative DNA damage. 47 They are considered direct markers for DNA damage and surrogate markers for ROS production in vivo. 47 Quantitative measurement of ROS in gastric tissues in vivo is difficult, but dihydroethidine staining has been utilized for qualitative studies in infected mice.…”

Section: Increased Expression Of Spermine Oxidase During H Pylori Inmentioning

confidence: 99%

“…47 They are considered direct markers for DNA damage and surrogate markers for ROS production in vivo. 47 Quantitative measurement of ROS in gastric tissues in vivo is difficult, but dihydroethidine staining has been utilized for qualitative studies in infected mice. 48 H. pylori causes an increase in ROS, both from immune cell infiltration and de novo synthesis by epithelial cells.…”

Section: Increased Expression Of Spermine Oxidase During H Pylori Inmentioning

confidence: 99%

See 1 more Smart Citation

Chronic inflammation and oxidative stress

et al. 2013

View full text Add to dashboard Cite

Helicobacter pylori is the leading risk factor associated with gastric carcinogenesis. H. pylori leads to chronic inflammation because of the failure of the host to eradicate the infection. Chronic inflammation leads to oxidative stress, deriving from immune cells and from within gastric epithelial cells. This is a main contributor to DNA damage, apoptosis and neoplastic transformation. Both pathogen and host factors directly contribute to oxidative stress, including H. pylori virulence factors, and pathways involving DNA damage and repair, polyamine synthesis and metabolism, and oxidative stress response. Our laboratory has recently uncovered a mechanism by which polyamine oxidation by spermine oxidase causes H 2O 2 release, DNA damage and apoptosis. Our studies indicate novel targets for therapeutic intervention and risk assessment in H. pylori-induced gastric cancer. More studies addressing the many potential contributors to oxidative stress, chronic inflammation, and gastric carcinogenesis are essential for development of therapeutics and identification of gastric cancer biomarkers.

show abstract

“…Within cancer research, the use of biomarkers is an important tool for the early detection of cancer and for determining prognosis. As DNA damage is a hallmark of carcinogenesis, signatures of DNA damage, specifically 8-oxoG, may serve as powerful biomarkers for gastric cancer [5,80]. 8-oxoG and 8-OHdG assays can measure nucleotide adducts that reflect oxidative DNA damage [80] and serve as direct markers of DNA damage and proxy markers for oxidative stress in vivo [14,21,80].…”

Section: Ape-1: Enzyme That Is Responsible For Creating a Nick In Thementioning

confidence: 99%

At the Bench:Helicobacter pylori, dysregulated host responses, DNA damage, and gastric cancer

Hardbower

Peek

Wilson

2014

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Helicobacter pylori infection is the strongest known risk factor for the development of gastric cancer. Given that ∼50% of the global population is infected with this pathogen, there is great impetus to elucidate underlying causes that mediate progression from infection to cancer. Recent evidence suggests that H. pylori-induced chronic inflammation and oxidative stress create an environment conducive to DNA damage and tissue injury. DNA damage leads to genetic instability and eventually, neoplastic transformation. Pathogen-encoded virulence factors induce a robust but futile immune response and alter host pathways that lower the threshold for carcinogenesis, including DNA damage repair, polyamine synthesis and catabolism, antioxidant responses, and cytokine production. Collectively, such dysregulation creates a protumorigenic microenvironment within the stomach. This review seeks to address each of these aspects of H. pylori infection and to call attention to areas of particular interest within this field of research. This review also seeks to prioritize areas of translational research related to H. pylori-induced gastric cancer based on insights garnered from basic research in this field. See related review by Dalal and Moss, At the Bedside: H. pylori, dysregulated host responses, DNA damage, and gastric cancer.

show abstract

Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

Cited by 82 publications

References 70 publications

One-year follow-up of clinical, metabolic and oxidative stress profile of morbid obese patients after laparoscopic sleeve gastrectomy. 8-oxo-dG as a clinical marker

One-year follow-up of clinical, metabolic and oxidative stress profile of morbid obese patients after laparoscopic sleeve gastrectomy. 8-oxo-dG as a clinical marker

Chronic inflammation and oxidative stress

At the Bench:Helicobacter pylori, dysregulated host responses, DNA damage, and gastric cancer

Contact Info

Product

Resources

About