Oxidative stress and cell death induction by amitraz and its metabolite BTS-27271 mediated through cytochrome P450 and NRF2 pathway alteration in primary hippocampal cell

Moyano, Paula; Ruíz, Matilde Carlón; García, José M.; Frejo, María Teresa; Anadón, M.J.; Lobo, Margarita; Pino, Javier del

doi:10.1016/j.fct.2019.04.042

Cited by 16 publications

(12 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This agrees with the function of peroxiredoxins to scavenge ROS, which can be elicited by exposure to many insecticides and plant defense allelochemicals [61,[54][55][56][66][67][68] and can reduce the fitness of insects [57,58]. This is also consistent with the findings that field-evolved imidacloprid resistance in N. lugens is mainly conferred by overexpression of the P450 genes CYP6ER1 and CYP6AY1 [40][41][42][43][44]69] and that P450s are known to be capable of generating ROS while metabolizing xenobiotics [62][63][64][69][70][71][72][73].…”

Section: Discussionsupporting

confidence: 89%

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

et al. 2021

View full text Add to dashboard Cite

Chemical insecticides have been heavily employed as the most effective measure for control of agricultural and medical pests, but evolution of resistance by pests threatens the sustainability of this approach. Resistance-conferring mutations sometimes impose fitness costs, which may drive subsequent evolution of compensatory modifier mutations alleviating the costs of resistance. However, how modifier mutations evolve and function to overcome the fitness cost of resistance still remains unknown. Here we show that overexpression of P450s not only confers imidacloprid resistance in the brown planthopper, Nilaparvata lugens, the most voracious pest of rice, but also leads to elevated production of reactive oxygen species (ROS) through metabolism of imidacloprid and host plant compounds. The inevitable production of ROS incurs a fitness cost to the pest, which drives the increase or fixation of the compensatory modifier allele T65549 within the promoter region of N. lugens peroxiredoxin (NlPrx) in the pest populations. T65549 allele in turn upregulates the expression of NlPrx and thus increases resistant individuals’ ability to clear the cost-incurring ROS of any source. The frequent involvement of P450s in insecticide resistance and their capacity to produce ROS while metabolizing their substrates suggest that peroxiredoxin or other ROS-scavenging genes may be among the common modifier genes for alleviating the fitness cost of insecticide resistance.

show abstract

Section: Discussionsupporting

confidence: 89%

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Imidacloprid toxicity was found to be dependent on the production of the detoxifying enzymes of the cytochrome P450 rather than just the diversity of detoxifying enzymes [ 19 ]. Adding that amitraz toxicity is affected by its metabolism by cytochrome P450 [ 51 ] implies that the effect of imidacloprid and amitraz co-exposure may be intrinsically different from the effect of single exposures.…”

Section: Discussionmentioning

confidence: 99%

Differential Production of Nitric Oxide and Hydrogen Peroxide among Drosophila melanogaster, Apis mellifera, and Mamestra brassicae Immune-Activated Hemocytes after Exposure to Imidacloprid and Amitraz

Sukkar

Laval-Gilly

Bonnefoy

et al. 2023

Insects

View full text Add to dashboard Cite

Invertebrates have a diverse immune system that responds differently to stressors such as pesticides and pathogens, which leads to different degrees of susceptibility. Honeybees are facing a phenomenon called colony collapse disorder which is attributed to several factors including pesticides and pathogens. We applied an in vitro approach to assess the response of immune-activated hemocytes from Apis mellifera, Drosophila melanogaster and Mamestra brassicae after exposure to imidacloprid and amitraz. Hemocytes were exposed to the pesticides in single and co-exposures using zymosan A for immune activation. We measured the effect of these exposures on cell viability, nitric oxide (NO) production from 15 to 120 min and on extracellular hydrogen peroxide (H2O2) production after 3 h to assess potential alterations in the oxidative response. Our results indicate that NO and H2O2 production is more altered in honeybee hemocytes compared to D. melanogaster and M. brassicae cell lines. There is also a differential production at different time points after pesticide exposure between these insect species as contrasting effects were evident with the oxidative responses in hemocytes. The results imply that imidacloprid and amitraz act differently on the immune response among insect orders and may render honeybee colonies more susceptible to infection and pests.

show abstract

“…Our results are in contrast with Radakovic et al (2013) findings, who showed that 3.5-μg/mL AMZ significantly increased catalase reaction with DNA damage and the percentage of apoptotic and necrotic cells in comparison with antioxidant catalase (100 IU/mL or 500 IU/mL). A recent study on mouse hippocampal cells culture found that AMZ induces oxidative stress from 80 to 100 μM, measured with different endpoints (cytochrome P450 isoenzymes, KEAP1/NRF2 pathway, cotreatment with the antioxidant N-acetylcysteine) (Moyano et al 2019). Many studies demonstrated that the concentration at which AMZ decreases cell viability depends on the model used (Ueng et al 2004;Young et al 2005;Padula et al 2012;Radakovic et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, Anchordoquy et al (2019b) observed that lymphocytes were more sensitive than CC to the antiparasitic doramectin. Since it is known that different mechanisms contribute to AMZ induce oxidative stress (Costa et al 1991;Moyano et al 2019), other mechanisms not investigated in this work could contribute to the generation of ROS and induce cell death, more studies are required to determine the cause of cell death in AMZ-treated CC. On the other hand, we cannot rule out that AMZ acts through another mechanism of action independently from the induction of ROS on bovine CC.…”

Section: Discussionmentioning

confidence: 99%

Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation

Nikoloff

Martín

Fabra

et al. 2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 50 24h ) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.

show abstract

Oxidative stress and cell death induction by amitraz and its metabolite BTS-27271 mediated through cytochrome P450 and NRF2 pathway alteration in primary hippocampal cell

Cited by 16 publications

References 44 publications

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

Differential Production of Nitric Oxide and Hydrogen Peroxide among Drosophila melanogaster, Apis mellifera, and Mamestra brassicae Immune-Activated Hemocytes after Exposure to Imidacloprid and Amitraz

Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation

Contact Info

Product

Resources

About