Oxidative stress and apoptosis was induced by bio-insecticide spinosad in the liver of Oreochromis niloticus

Piner, Petek; Üner, Nevin

doi:10.1016/j.etap.2013.08.009

Cited by 30 publications

(13 citation statements)

References 52 publications

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“…The reduction in the levels of MDA in the present study is not in agreement with the study performed by Mozhdeganloo et al ( 2015 ). The decrease in the GSH/GSSG ratio for 0.5 mg/L MT exposure groups in the present study, implied the oxidation of GSH to GSSG with the signal of increased detoxification of ROS (Guzmán-Guillén et al 2013 ), which was the same as tilapia liver under spinosad exposure (Piner and Üner 2013 ).…”

Section: Discussionsupporting

confidence: 61%

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

et al. 2016

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The normal dose of 17α-methyltestosterone (MT) used in fish farming was 60 mg/L, and now the analysis of residual androgens was carried out in waste water obtained from the Beijing area, which could be detected in levels ranging from 4.1 to 7.0 ng/L. For the purpose of aquatic early warning, the present study clearly demonstrated that chronic exposure by higher concentration of MT than environmental relevant concentrations could trigger oxidative stress response to juvenile tilapia by modulating hepatic antioxidant enzyme activities and gene transcription. Some antioxidative parameters (T-GSH, GSH/GSSG and MDA) were significant decreased under 0.5 mg/L MT exposure at 7 and 14 days. Some antioxidant enzymes (SOD, CAT and GST) and transcriptional changes (sod and cat) were revealed significant decreases for MT treated groups at 7 days. Total antioxidant capacity was significant increased only in 5 mg/L MT exposure groups, but GR activities were not affected all through the whole exposure period. Almost all of the antioxidant enzymatic genes detected in the present study were showed significant increments for MT exposure both at 14 and 21 days, and the genotoxicity profile of antioxidant enzymatic genes were revealed dose-dependent manner. This study presented evidence that MT could result in oxidative stress response in the early stages of GIFT tilapia.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-1946-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 61%

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

et al. 2016

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“…GSH/GSSG was significantly decreased (2.13-fold) and increased (1.25-fold) for 0.5 and 5 mg/L PCB-exposure groups, respectively, at 7 days (Table 1). Increased detoxification of ROS implies the oxidation of GSH to GSSG (Guzmán-Guillén et al, 2013), which was similar to results observed for tilapia livers following spinosad exposure (Piner and Üner, 2013). The alteration of GSH content and metabolism in different studies suggest that GSH has a key role in oxidative-induced toxicity caused by PCBs.…”

Section: Resultssupporting

confidence: 72%

“…Furthermore, mRNA expression of antioxidant genes (sod, cat, and gpx1) was strongly enhanced in the Chinese rare minnow (Gobiocypris rarus) by Aroclor 1254 (a highly chlorinated PCB mixture Wu et al, 2014a). In addition to frequently used oxidative stress markers (SOD, CAT, and GPx), the GSH-related antioxidant system attempted to protect the liver cells of Oreochromes niloticus from hepatotoxicity (Piner and Üner, 2013), and markers in this system include GST, GR activity, GSH content, and the GSH/GSSG ratio among others. Genetic Improvement of Farmed Tilapia (GIFT), O. niloticus, is sensitive to the stress of pollutants and is an ideal subject for toxicity experiments (Piner and Üner, 2013;Meng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to frequently used oxidative stress markers (SOD, CAT, and GPx), the GSH-related antioxidant system attempted to protect the liver cells of Oreochromes niloticus from hepatotoxicity (Piner and Üner, 2013), and markers in this system include GST, GR activity, GSH content, and the GSH/GSSG ratio among others. Genetic Improvement of Farmed Tilapia (GIFT), O. niloticus, is sensitive to the stress of pollutants and is an ideal subject for toxicity experiments (Piner and Üner, 2013;Meng et al, 2014). The main purpose of the present study was to investigate the hepatic genotoxicity (transcriptional) and antioxidant enzymatic signature (post-transcriptional) in juvenile freshwater tilapia, O. niloticus, in response to 0.5 or 5 mg/L PCB exposure.…”

Section: Introductionmentioning

confidence: 99%

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Effect of polychlorinated biphenyls on oxidation stress in the liver of juvenile GIFT, Oreochromis niloticus

et al. 2016

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“…Owing to its highly selective toxicological profile, spinosad is widely used for control of crop and amenity plants, public hygiene, and veterinary pests (Böhm et al, 2014;Mandal, Singh, Battu, & Singh, 2013;Markussen & Kristensen, 2012). There is considerable evidence that spinosad may have oxidative damage by compromise antioxidant systems, excess reactive oxygen species (ROS) production and induce lipid peroxidation (Ahmed 2012;Pérez-Pertejo, Reguera, Ordóñez, & Balaña-Fouce, 2008;Piner & Üner, 2013Piner & Üner, , 2014. Furthemore, several studies have reported that spinosad exposure may cause DNA fragmentation and chromosomal aberration in rats (Ahmed 2012;Mansour, Mossa, & Heikal, 2008).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

Yang

Gao

et al. 2017

Food and Agricultural Immunology

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Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA damage were measured. The results showed that spinosad caused a time-dependent increase in the formation of malondialdehyde and decrease in the activity of superoxide dismutase and catalase. Further studies confirmed that spinosad induced 8-oxoguanine accumulation in Sf9 cells, which is accompanied by increased expression of DNA repair enzymes (OGG1 and MTH1). The neutral comet assay revealed that spinosad induced significant timerelated increases of DNA double-strand breaks in Sf9 cells. Our results indicate that spinosad effectively induced oxidative stress and DNA damage in Sf9 cells. ARTICLE HISTORY

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Oxidative stress and apoptosis was induced by bio-insecticide spinosad in the liver of Oreochromis niloticus

Cited by 30 publications

References 52 publications

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

Effect of polychlorinated biphenyls on oxidation stress in the liver of juvenile GIFT, Oreochromis niloticus

Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

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