Oxidative stress and antioxidants: Distress or eustress ?

Niki, Etsuo

doi:10.1016/j.abb.2015.11.017

Cited by 156 publications

(100 citation statements)

References 93 publications

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“…The resulting residue was purified using flash chromatography and eluted with hexanes/ethyl acetate (3:2) affording an orange solid (47 mg, 11%). 1 Trolox (0.026 g, 0.1 mmol, 1 eq) and H 4 MeBOH (0.018 g, 0.07 mmol, 0.7 eq) were dissolved in dry THF (1 mL) under argon. Dimethylaminopyridine (0.0024 g, 0.02 mmol, 0.2 eq) and N,N 0 -Diisopropylcarbodiimide (0.016 mL, 0.1 mmol, 1 eq) were then added.…”

Section: Methodsmentioning

confidence: 99%

“…The solution was stirred for 1 h at room temperature after which the solvent was removed under reduced pressure. The resulting residue was purified using flash column chromatography and eluted with hexanes/ethyl acetate (3:2) affording a pink solid (8 mg, 24%) 1 • 8-((AE)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carbonyloxy)-methyl-2,6-dichloro-1,3,5,7-tetramethyl pyrromethene fluoroborate, (Cl 2 BTOH). Trolox (0.024 g, 0.1 mmol, 1 eq) and Cl 2 BOH (0.018 g, 0.08 mmol, 0.8 eq) were dissolved in dry THF (1 mL) under argon.…”

Section: Methodsmentioning

confidence: 99%

“…The solution was stirred for 1.5 h at room temperature after which the solvent was removed under reduced pressure. The resulting residue was purified using flash column chromatography and eluted with hexanes/ethyl acetate (3:2) affording a pink solid (36.5 mg, 81%) 1 0009 mol) was dissolved in thionyl chloride (2 mL) under argon and stirred at 50°C for 1 h. The thionyl chloride was then removed by coevaporation with toluene (15 mL, five times), and the flask was left on a vacuum pump overnight. The resulting acid chloride was then dissolved in 10 mL of dichloromethane under argon.…”

Section: Methodsmentioning

confidence: 99%

“…Reactive oxygen species (ROS) and their detrimental/beneficial effects have been greatly debated recently (1). Crucial to unraveling the specific roles and implications of ROS in various pathologies (2,3) and physiologies (4) are tools that may report the evolution of ROS concentration in both time and space coordinates in live cells and in a noninvasive manner.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tuning Photoinduced Electron Transfer Efficiency of Fluorogenic BODIPY‐α‐Tocopherol Analogues

Greene

Lincoln

Cosa

2018

Photochem & Photobiology

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Fluorogenic analogues of α‐tocopherol developed by our group have been instrumental in monitoring reactive oxygen species (ROS) within lipid membranes. Prepared as two‐segment trap‐reporter (chromanol‐BODIPY) probes, photoinduced electron transfer (PeT) was utilized to provide these probes with an off/on switch mechanism warranting the necessary sensitivity. Herein, we rationalize within the context of Marcus theory of electron transfer how substituents on the BODIPY core and linker length joining the trap and reporter segments, tune PeT efficiency. DFT and electrochemical studies were used to estimate the thermodynamic driving force of PeT in our constructs. By tuning the redox potential over a 400 mV range, we observed over an order of magnitude increase in PeT efficiency. Increasing the linker length between the chromanol and BODIPY by 2.8 angstroms, in turn, decreased PeT efficiency 2.7‐fold. Our results illustrate how substituent and linker choice enable “darkening” the off state of fluorogenic probes based on BODIPY fluorophores, by favoring PeT over radiative emission from the singlet excited state manifold. Ultimately, our work brings light to the sensitivity ceiling one may achieve in developing fluorogenic antioxidant analogues of α‐tocopherol. The work provides general guidelines applicable to those developing fluorogenic probes based on PeT.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tuning Photoinduced Electron Transfer Efficiency of Fluorogenic BODIPY‐α‐Tocopherol Analogues

Greene

Lincoln

Cosa

2018

Photochem & Photobiology

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“…It serves as a key molecule in the Third Principle of the Redox Code, which is: “Redox sensing through activation/deactivation cycles of H 2 O 2 production linked to the NAD and NADP systems to support spatiotemporal organisation of key processes” [6]·H 2 O 2 occurs in normal metabolism in mammalian cells [7] and is a key metabolite in oxidative stress (see [8]). The term “oxidative eustress” [9], [10], which denotes physiological oxidative stress, may serve in the distinction from excessive load, “oxidative distress”, causing oxidative damage (see also [11], [12], [13], [14]). The general concept of eustress vs .…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

2017

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Hydrogen peroxide emerged as major redox metabolite operative in redox sensing, signaling and redox regulation. Generation, transport and capture of H2O2 in biological settings as well as their biological consequences can now be addressed. The present overview focuses on recent progress on metabolic sources and sinks of H2O2 and on the role of H2O2 in redox signaling under physiological conditions (1–10 nM), denoted as oxidative eustress. Higher concentrations lead to adaptive stress responses via master switches such as Nrf2/Keap1 or NF-κB. Supraphysiological concentrations of H2O2 (>100 nM) lead to damage of biomolecules, denoted as oxidative distress. Three questions are addressed: How can H2O2 be assayed in the biological setting? What are the metabolic sources and sinks of H2O2? What is the role of H2O2 in redox signaling and oxidative stress?

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Ozone at low concentration modulates microglial activity in vitro: A multimodal microscopy and biomolecular study

Lacavalla

Inguscio

Cisterna

et al. 2022

Microscopy Res & Technique

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Oxygen-ozone (O 2 -O 3 ) therapy is an adjuvant/complementary treatment based on the activation of antioxidant and cytoprotective pathways driven by the nuclear factor erythroid 2-related factor 2 (Nrf2). Many drugs, including dimethyl fumarate (DMF), that are used to reduce inflammation in oxidative-stress-related neurodegenerative diseases, act through the Nrf2-pathway. The scope of the present investigation was to get a deeper insight into the mechanisms responsible for the beneficial result of O 2 -O 3 treatment in some neurodegenerative diseases. To do this, we used an integrated approach of multimodal microscopy (bright-field and fluorescence microscopy, transmission and scanning electron microscopy) and biomolecular techniques to investigate the effects of the low O 3 concentrations currently used in clinical practice in lipopolysaccharide (LPS)-activated microglial cells human microglial clone 3 (HMC3) and in DMF-treated LPS-activated (LPS + DMF) HMC3 cells. The results at light and electron microscopy showed that LPSactivation induced morphological modifications of HMC3 cells from elongated/ branched to larger roundish shape, cytoplasmic accumulation of lipid droplets, decreased electron density of the cytoplasm and mitochondria, decreased amount of Nrf2 and increased migration rate, while biomolecular data demonstrated that Heme oxygenase 1 gene expression and the secretion of the pro-inflammatory cytokines, Interleukin-6, and tumor necrosis factor-α augmented. O 3 treatment did not affect cell viability, proliferation, and morphological features of both LPS-activated and LPS + DMF cells, whereas the cell motility and the secretion of proinflammatory cytokines were significantly decreased. This evidence suggests that modulation of microglia activity may contribute to the beneficial effects of the O 2 -O 3 therapy in patients with neurodegenerative disorders characterized by chronic inflammation.

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Oxidative stress and antioxidants: Distress or eustress ?

Cited by 156 publications

References 93 publications

Tuning Photoinduced Electron Transfer Efficiency of Fluorogenic BODIPY‐α‐Tocopherol Analogues

Tuning Photoinduced Electron Transfer Efficiency of Fluorogenic BODIPY‐α‐Tocopherol Analogues

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

Ozone at low concentration modulates microglial activity in vitro: A multimodal microscopy and biomolecular study

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