Oxidative stress and antioxidant response in rainbow trout fry exposed to acute hypoxia is affected by selenium nutrition of parents and during first exogenous feeding

Wischhusen, Pauline; Larroquet, Laurence; Durand, Thierry; Oger, Camille; Galano, Jean‐Marie; Rocher, Amandine; Vigor, Claire; Prabhu, P. Antony Jesu; Véron, Vincent; Briens, Mickaël; Roy, Jérôme; Kaushik, Sadasivam; Fauconneau, Benoı̂t; Fontagné-Dicharry, Stéphanie

doi:10.1016/j.freeradbiomed.2020.05.006

Cited by 28 publications

(22 citation statements)

References 53 publications

(71 reference statements)

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“…Regarding the transmethylation pathway, similar to normoxic fry, in stressed fry bhmt mRNA levels were lower when originating from broodstock fed the inorganic Se-supplemented diet Bss. This persistent effect by parental Se suggests a strong nutritional programming effect on the methionine cycling, which is similar to the effects we observed earlier in the antioxidant system, where a transcriptional downregulation in gene expression was observed in stressed fry originating from parents fed sodium selenite supplemented diets (10) .…”

Section: Impact Of Selenium On the 1c Metabolism In Stressed Rainbow supporting

confidence: 87%

“…On the other hand, these fry presented lower total body glutathione (GSH) levels leading to the hypothesis that parental Se might relate to epigenetic modifications at early stages possibly reflecting a phenomenon known as nutritional programming (9) . In a follow-up study it was confirmed that in the progeny even after the beginning of exogenous feeding parental Se supplementation decreased the expression of genes related to the GSH metabolism such as GSH peroxidase and GSH reductase (10) . This effect was especially strong after the fish were exposed to a hypoxic stress, which has been associated with an increase in cellular production of reactive oxygen species and oxidative stress levels in fish (11,12) .…”

Section: Introductionmentioning

confidence: 82%

“…The sample size was determined using power analysis (R: pwr2, version 1.0) with a medium expected effect size for gene expression data (n=27) and a large expected effect size for other parameters (n=9) based on previous studies. (10) The statistical power was set to a minimum of 80% at a significance level of p < 0.05 in all analyses. Data were tested for homogeneity and normal distribution resulting in rank transformation of gene expression data before further analysis.…”

Section: Discussionmentioning

confidence: 99%

“…At the end of the 11-week feeding period (average final body weight: 4.6 ± 0.1 g), the fish were either sampled or subjected to a hypoxic stress challenge as previously described (10) .…”

Section: Donzacqmentioning

confidence: 99%

“…The mRNA was extracted from three randomly chosen individual fry per tank (=nine individuals per dietary treatment) and analyzed by high-throughput qRT-PCR at the GenotoulPlatform (http://genomique.genotoul.fr/) using the BioMark ® 96:96 Dynamic Array (Fluidigm Corporation, San Francisco, CA, USA) as previously described (10) . The primer sequences are given in Table 2.…”

Section: Gene Expressionmentioning

confidence: 99%

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Long-term effect of parental selenium supplementation on the one-carbon metabolism in rainbow trout (Oncorhynchus mykiss) fry exposed to hypoxic stress

et al. 2021

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This study evaluated how different forms of Se supplementation into rainbow trout broodstock diets modified the one-carbon metabolism of the progeny after the beginning of exogenous feeding and followed by hypoxia challenge. The progeny of three groups of rainbow trout broodstock fed either a control diet (Se level: 0.3 µg/g) or a diet supplemented with inorganic sodium selenite (Se level: 0.6 µg/g) or organic hydroxy-selenomethionine (Se level: 0.6 µg/g) were cross-fed with diets of similar Se composition for 11 weeks. Offspring were sampled either before or after being subjected to an acute hypoxic stress (1.7 mg/L dissolved oxygen) for 30 minutes. In normoxic fry, parental Se supplementation allowed higher glutathione levels compared to fry originating from parents fed the control diet. Parental hydroxy-selenomethionine treatment also increased cysteine and cysteinyl-glycine concentrations in fry. Dietary Se supplementation decreased cgl mRNA levels. Hydroxy-selenomethionine feeding also lowered the levels of some essential free amino acids in muscle tissue. Supplementation of organic Se to parents and fry reduced bhmt expression in fry. The hypoxic stress decreased whole-body homocysteine, cysteine, cysteinyl-glycine and glutathione levels. Together with the higher mRNA levels of cbs, a transsulfuration enzyme, this suggests that under hypoxia, glutathione synthesis through transsulfuration might have been impaired by the depletion of a glutathione precursor. In stressed fry, S-adenosylmethionine levels were significantly decreased, but S-adenosylhomocysteine remained stable. Decreased bhmt and amd1a mRNA levels in stressed fry suggest a nutritional programming by parental Se also on methionine metabolism of rainbow trout.

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Section: Impact Of Selenium On the 1c Metabolism In Stressed Rainbow supporting

confidence: 87%

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

“…At the end of the 11-week feeding period (average final body weight: 4.6 ± 0.1 g), the fish were either sampled or subjected to a hypoxic stress challenge as previously described (10) .…”

Section: Donzacqmentioning

confidence: 99%

Section: Gene Expressionmentioning

confidence: 99%

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