Oxidative stress and aging: Learning from yeast lessons

Eleuthério, Elis C. A.; Brasil, Aline de Araújo; França, Mauro Braga; Almeida, Diego S. G. de; Rona, Germana Breves; Magalhães, Rayne Stfhany Silva

doi:10.1016/j.funbio.2017.12.003

Cited by 42 publications

(27 citation statements)

References 164 publications

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“…Removal of water by dry-air and elevated temperatures generate modifications in the water potential of the medium and in the cells themselves, leading to a decrease in their volume and an increase of their contact surface with air (Beker and Rapoport, 1987;Gervais and Beney, 2001;Lemetais et al, 2012;Rapoport et al, 2016). All these modifications can induce the accumulation of reactive oxygen species (ROS), consequently, oxidative stress and cause the cells death (Eleutherio et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

et al. 2020

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During industrial yeast production, cells are often subjected to deleterious hydric variations during dehydration, which reduces their viability and cellular activity. This study is focused on the yeast Lachancea thermotolerans, particularly sensitive to dehydration. The aim was to understand the modifications of single-cells biophysical profiles during different dehydration conditions. Infrared spectra of individual cells were acquired before and after dehydration kinetics using synchrotron radiation-based Fourier-transform infrared (S-FTIR) microspectroscopy. The cells were previously stained with fluorescent probes in order to measure only viable and active cells prior to dehydration. In parallel, cell viability was determined using flow cytometry under identical conditions. The S-FTIR analysis indicated that cells with the lowest viability showed signs of membrane rigidification and modifications in the amide I (α-helix and β-sheet) and amide II, which are indicators of secondary protein structure conformation and degradation or disorder. Shift of symmetric C-H stretching vibration of the CH 2 group upon a higher wavenumber correlated with better cell viability, suggesting a role of plasma membrane fluidity. This was the first time that the biophysical responses of L. thermotolerans single-cells to dehydration were explored with S-FTIR. These findings are important for clarifying the mechanisms of microbial resistance to stress in order to improve the viability of sensitive yeasts during dehydration.

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Section: Introductionmentioning

confidence: 99%

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

et al. 2020

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“…A severe oxidative stress can lead to a massive ROS production possibly followed by cell death [ 40 ]. S. cerevisiae plays a key role in understanding of the relationships between aging and the non-homeostatic ROS production, given that the mammalian and yeast antioxidant responses are similar [ 41 ]. So, it appeared relevant to study the response of HCA-treated aging yeast cells to H 2 O 2 induced oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

In S. cerevisiae hydroxycitric acid antagonizes chronological aging and apoptosis regardless of citrate lyase

et al. 2020

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Caloric restriction mimetics (CRMs) are promising molecules to prevent age-related diseases as they activate pathways driven by a true caloric restriction. Hydroxycitric acid (HCA) is considered a bona fide CRM since it depletes acetyl-CoA pools by acting as a competitive inhibitor of ATP citrate lyase (ACLY), ultimately repressing protein acetylation and promoting autophagy. Importantly, it can reduce inflammation and tumour development. In order to identify phenotypically relevant new HCA targets we have investigated HCA effects in Saccharomyces cerevisiae, where ACLY is lacking. Strikingly, the drug revealed a powerful anti-aging effect, another property proposed to mark bona fide CRMs. Chronological life span (CLS) extension but also resistance to acetic acid of HCA treated cells were associated to repression of cell apoptosis and necrosis. HCA also largely prevented cell deaths caused by a severe oxidative stress. The molecule could act widely by negatively modulating cell metabolism, similarly to citrate. Indeed, it inhibited both growth reactivation and the oxygen consumption rate of yeast cells in stationary phase. Genetic analyses on yeast CLS mutants indicated that part of the HCA effects can be sensed by Sch9 and Ras2, two conserved key regulators of nutritional and stress signal pathways of primary importance. Our data together with published biochemical analyses indicate that HCA may act with multiple mechanisms together with ACLY repression and allowed us to propose an integrated mechanistic model as a basis for future investigations.

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“…There are as yet few similar investigations on vertebrate cells including human; it would therefore be of great interest to confirm the data obtained in Saccharomyces cerevisiae. Actually, it has been reported that 25% of genes related to human degenerative pathologies overlap almost completely with those of yeast pathologies, thus allowing the study of homologous antioxidant response genes in much simpler eukaryotic organisms (46).…”

Section: Superoxide Dismutasementioning

confidence: 99%

Copper/Zinc Superoxide Dismutase in Human Skin: Current Knowledge

et al. 2020

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Superoxide dismutase is widespread in the human body, including skin and its appendages. Here, we focus on human skin copper/zinc superoxide dismutase, the enzyme that protects skin and its appendages against reactive oxygen species. Human skin copper/zinc superoxide dismutase resides in the cytoplasm of keratinocytes, where up to 90% of cellular reactive oxygen species is produced. Factors other than cell type, such as gender, age and diseased state influence its location in skin tissues. We review current knowledge of skin copper/zinc superoxide dismutase including recent studies in an attempt to contribute to solving the question of its remaining unexplained functions. The research described here may be applicable to pathologies associated with oxidative stress. However, recent studies on copper/zinc superoxide dismutase in yeast reveal that its predominant function may be in signaling pathways rather than in scavenging superoxide ions. If confirmed in the skin, novel approaches might be developed to unravel the enzyme's remaining mysteries.

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Oxidative stress and aging: Learning from yeast lessons

Cited by 42 publications

References 164 publications

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

In S. cerevisiae hydroxycitric acid antagonizes chronological aging and apoptosis regardless of citrate lyase

Copper/Zinc Superoxide Dismutase in Human Skin: Current Knowledge

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