“…Thiol groups, which are part of the NPSH, protect sperm cells against oxidative stress (Bansal and Bilaspuri, 2008). The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with diluent when compared to the group supplemented with Trolox (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Membrane sulfhydryl groups serve as an important defense mechanism of the spermatozoa to fight against oxidative stress. Reduction in motility and losses of sperm functions can mask the activity of sulfhydryl groups and can be associated with male infertility (Bansal and Kaur, 2009;Bansal and Bilaspuri, 2008;Nivsarkar et al, 1998). In addition to that, the nonprotein thiols play a significant role in eliminating toxic compounds (Seligman et al, 2005).…”
Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.
“…Thiol groups, which are part of the NPSH, protect sperm cells against oxidative stress (Bansal and Bilaspuri, 2008). The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with diluent when compared to the group supplemented with Trolox (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Membrane sulfhydryl groups serve as an important defense mechanism of the spermatozoa to fight against oxidative stress. Reduction in motility and losses of sperm functions can mask the activity of sulfhydryl groups and can be associated with male infertility (Bansal and Kaur, 2009;Bansal and Bilaspuri, 2008;Nivsarkar et al, 1998). In addition to that, the nonprotein thiols play a significant role in eliminating toxic compounds (Seligman et al, 2005).…”
Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.
“…[20] This fact could be justified by an increase in the amount of glutathione, a peptide with antioxidant functions through its cysteine residue. [21,22] This increase could reveal that the semen sample is under oxidant conditions caused by acute lifestyle changes and that the organism is producing more antioxidants as a defense mechanism. However, the CO groups revealed no statistical differences between TP1 and TP2 ( Figure 4, 5).…”
Objective: To study the effects of an acute lifestyle change in human semen oxidative stress (OS) by applying seminal parameters and OS markers and to study the feasibility of mid-infrared spectroscopy with Fourier transform infrared spectroscopy (FT-IR) as a complementary tool to evaluate the effects of OS on human sperm samples.
Material and methods:Sperm samples were collected from healthy male students (n=8) who voluntarily submitted themselves to acute lifestyle changes during academic festivities. The samples were obtained before and after the academic festivities and were compared by basic semen analyses and OS markers, namely with thiobarbituric acid reactive species (TBARS) and total thiol (SH) groups by spectrophotometric assays and carbonyl (CO) groups by slot blot. The samples were also submitted for spectroscopic analysis to evaluate the feasibility of FT-IR coupled with multivariate analysis to calibrate OS biomarkers. Statistical analysis was performed applying paired Wilcoxon tests.Results: Acute lifestyle alterations during academic week festivities were associated with a significant decrease in the percentage of normal spermatozoa in the ejaculate (p=0.011) and a decrease in sperm concentration and in semen volume. Regarding OS, acute lifestyle changes promoted a significant increment of TBARS (p=0.018) and an increasing trend in the SH group. With FT-IR and multivariate analysis, it was possible to develop calibration models to the following protein OS biomarkers: SH groups and CO.Conclusions: Acute lifestyle changes during academic festivities have negative effects on sperm quality, in both conventional seminal parameters and OS markers. The evaluation of OS biomarkers and FT-IR could improve andrology diagnosis and therapeutic follow-up.
“…Enzymatic antioxidants are composed of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione reductase (GR) [66]. SOD spontaneously dismutase • generated by NADPH oxidase in neutrophils and play a major role in decreasing LPO and protecting spermatozoa against oxidative damage [64]. Catalase is mainly found in ram and cattle spermatozoa, and its potential role is to control oxidative stress caused by H 2 O 2 , , and thus, it prevents aging process in sperm [43].…”
Section: Enzymatic Antioxidantsmentioning
confidence: 99%
“…These groups serve as defense mechanisms of sperm cells to fight against oxidative stress [62]. A variety of biological and chemical antioxidants that attack ROS and LPO are presently under investigation [64]. Diluter supplemented with antioxidant or combination of antioxidants prior to the cryopreservation process may be recommended to facilitate the enhancement of sperm cryopreservation technique for the goat breeding industry [36].…”
Oxidative stress (OS) has been considered a major contributory factor to the male infertility. It is the result of imbalance between the reactive oxygen species (ROS) and antioxidants in the body which can lead to sperm damage, deformity, and eventually male infertility. Although high concentrations of the ROS cause sperm pathology (ATP depletion) leading to insufficient axonemal phosphorylation, lipid peroxidation, and loss of motility and viability, but many evidences demonstrate that low and controlled concentrations of these ROS play an important role in sperm physiological processes such as capacitation, acrosome reaction, and signaling processes to ensure fertilization. ROS are also generated during cryopreservation of spermatozoa for AI practices. To reduce the oxidative stress, there are certain compounds and reactions which dispose, scavenge, and suppress the formation of ROS or oppose their actions are called antioxidants. Currently, many antioxidants are under investigation. The supplementation of a cryopreservation extender with antioxidant has been shown to provide a cryoprotective effect on mammalian sperm quality. This chapter explains the impacts of oxidative stress and reactive oxygen species on spermatozoa functions, causes of ROS generation, and antioxidative strategies to reduce this OS. This study also suggests that antioxidant supplementation could be of clinical importance in prolonging the spermatozoal storage for assisted reproductive techniques (ARTs) like artificial insemination (AI), in vitro fertilization (IVF), and intrauterine insemination (IUI) purposes.
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