Oxidative release of natural glycans for functional glycomics

Song, Xuezheng; Ju, Hong; Lasanajak, Yi; Kudelka, Matthew R; Smith, David F.; Cummings, Richard D.

doi:10.1038/nmeth.3861

Cited by 157 publications

(176 citation statements)

References 74 publications

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“…While considering the advantages of the simplification of glycan profile following the core GalNAcol splitting after the mild periodate oxidation, mention is required that this is at the expense of much of the natural O-glycan core which is largely sacrificed. Work is under way to develop further and broaden the beam search technology to encompass core-intact O-glycans released by a recently described method (38). This will serve to identify core-specific recognition which may be missed following the core-split.…”

Section: Discussionmentioning

confidence: 99%

O-Glycome Beam Search Arrays for Carbohydrate Ligand Discovery

Gao

Zhang

et al. 2018

Molecular & Cellular Proteomics

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O-glycosylation is a post-translational modification of proteins crucial to molecular mechanisms in health and disease. O-glycans are typically highly heterogeneous. The involvement of specific O-glycan sequences in many bio-recognition systems is yet to be determined because of a lack of efficient methodologies. We describe here a targeted microarray approach: O-glycome beam search that is both robust and efficient for O-glycan ligand-discovery. Substantial simplification of the complex O-glycome profile and facile chromatographic resolution is achieved by arraying O-glycans as branches, monitoring by mass spectrometry, focusing on promising fractions, and on-array immuno-sequencing. This is orders of magnitude more sensitive than traditional methods. We have applied beam search approach to porcine stomach mucin and identified extremely minor components previously undetected within the O-glycome of this mucin that are ligands for the adhesive proteins of two rotaviruses. The approach is applicable to O-glycome recognition studies in a wide range of biological settings to give insights into glycan recognition structures in natural microenvironments. Molecular & Cellular

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Section: Discussionmentioning

confidence: 99%

O-Glycome Beam Search Arrays for Carbohydrate Ligand Discovery

Gao

Zhang

et al. 2018

Molecular & Cellular Proteomics

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“…Techniques capable of isolating such small amounts of samples are required, in addition to new preparation methods. Although not included in this review, the recent advances in deriving glycan samples from glycoproteins and glycolipids and their use in the biological investigations are also worth mentioning [88,89].…”

Section: Resultsmentioning

confidence: 99%

Addressing the glycan complexity by using mass spectrometry: In the pursuit of decoding glycologic

Kanie¹,

Kanie²

2017

Bio Chem Comp

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Biomolecules often contain carbohydrates. Such molecules, namely glycoconjugates, play central roles in cell adhesion, tumor migration, and attachment of pathogens. The conjugation of "natural products" with glycans is not a template-dependent process but is achieved by multiple enzymatic reactions. Because of the nature of the synthetic process, glycans exist as a complex mixture creating a glycoform and thus, the analysis becomes inevitably difficult. Mass spectrometry is one of the most informative, and thus important, tools for the structural analysis of glycans. The obvious advantage of mass spectrometry is the high sensitivity at low femtomole detection levels. A variety of ions can be obtained depending on the adducted cationic species. In order to obtain sequential information, the gas-phase dissociation reaction is used. Among the various techniques, collision-induced dissociation (CID) has been frequently used in the past. Although the technique is proven useful, there are cases that normal CID process is not suitable. This review highlights an emerging technique that focuses on the activation-energy difference between the isomeric glycans, and will complement the current methods. Furthermore, the possible source and the methodology for obtaining useful structural information are discussed.

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“…Reprinted with permission from Song, X.; Ju, H.; Lasanajak, Y.; Kudelka, M. R.; Smith, D. F.; Cummings, R. D., Oxidative release of natural glycans for functional glycomics. Nat Meth 2016, 13 (6), 528–534 ref(27). (Permission pending).…”

Section: Figurementioning

confidence: 99%

“…recently developed a method to simultaneously release free reducing N- and O-glycans from glycoproteins, and glycan nitriles from glycosphingolipids using household bleach. 27 (Figure 3) The free glycans are generally analyzed using either MALDI-MS or LC-MS. Alternatively, glycoproteins can be analyzed as glycopeptides through protease digestion of the protein, thereby keeping a segment of the peptide backbone intact.…”

Section: Introductionmentioning

confidence: 99%