Oxidative phosphorylation in guinea pig mammary gland mitochondria during various functional states

Nelson, Walter L.; Butow, Ronald A.; Ciaccio, Edward I.

doi:10.1016/0003-9861(62)90327-2

Cited by 19 publications

(11 citation statements)

References 11 publications

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“…The changes in mammary mitochondrial ATP synthesis activity that were observed in this study were consistent with previous observations on mammary mitochondrial respiration in lactating guinea pigs (44). In addition, the decrease of mammary mitochondrial ATP synthesis on day 21 postpartum correlates with previously reported decreases in the milk production, as assessed by litter gain and with increases in mammary apoptosis (26).…”

Section: Discussionsupporting

confidence: 92%

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Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Hadsell

Olea

Wei

et al. 2011

Physiological Genomics

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Hadsell DL, Olea W, Wei J, Fiorotto ML, Matsunami RK, Engler DA, Collier RJ. Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle. Physiol Genomics 43: 271-285, 2011. First published December 28, 2010 doi:10.1152/physiolgenomics.00133.2010.-The regulation of mitochondrial biogenesis and function in the lactating mammary cell is poorly understood. The goal of this study was to use proteomics to relate temporal changes in mammary cell mitochondrial function during lactation to changes in the proteins that make up this organelle. The hypothesis tested was that changes in mammary cell mitochondrial biogenesis and function during lactation would be accounted for by coordinated changes in the proteins of the electron transport chain and that some of these proteins might be linked by their expression patterns to PPARGC1␣ and AMP kinase. The mitochondrial proteome was studied along with markers of mitochondrial biogenesis and function in mammary tissue collected from mice over the course of a single prolonged lactation cycle. Mammary tissue concentrations of AMP and ADP were increased (P Ͻ 0.05) during early lactation and then declined with prolonged lactation. Similar changes were also observed for mitochondrial ATP synthesis activity, mitochondrial mass and DNA copy number. Analysis of the mammary cell mitochondrial proteome identified 244 unique proteins. Of these, only two proteins of the electron transport chain were found to increase during early lactation. In contrast, coordinated changes in numerous electron transport chain proteins were observed both during mid-and late lactation. There were six proteins that could be directly linked to PPARGC1␣ through network analysis. Abundance of PPARGC-1␣ and phosphorylation of AMP kinase was highest on day 2 postpartum. The results suggest that the increases in mammary mitochondria ATP synthesis activity during early lactation results from changes in only a limited number proteins. In addition, decreases in a handful of proteins linked to lipid oxidation could be temporally linked to decreases in PPARGC1␣ and phospho-AMP kinase suggesting potential roles for these proteins in coordinating mammary gland metabolism during early lactation. mitochondria; proteome; adenosine 5=-triphosphate

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Section: Discussionsupporting

confidence: 92%

“…In the lactating mammary gland, previous work has highlighted the importance of developmental changes in mitochondrial respiration with the onset of lactation and with mammary involution (17,44). In addition to this, mammary mitochondria have been suggested to play an important role in the provision of citrate for milk lipid biosynthesis (15,54,70).…”

mentioning

confidence: 99%

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Hadsell

Olea

Wei

et al. 2011

Physiological Genomics

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“…In the mouse, mitochondrial numbers increase dramatically during lactogenesis (Rosano et al, 1976). In addition, work in the guinea pig has demonstrated that oxidative phosphorylation activity of the mitochondria is increased with the onset of lactation (Nelson et al, 1962). Our recent work in the lactating mouse found that both ATP synthesis activity and the number of mitochondria per cell are coordinately increased with the potential for milk production during early lactation, and these markers decline with progression into late lactation (Hadsell et al, 2011).…”

mentioning

confidence: 87%

Milk yield differences between 1× and 4× milking are associated with changes in mammary mitochondrial number and milk protein gene expression, but not mammary cell apoptosis or SOCS gene expression

Alex

Collier

Hadsell

et al. 2015

Journal of Dairy Science

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Milking frequency is known to affect milk production and lactation persistence in dairy cows. Despite this, the mechanisms underlying this effect are only partially understood. Previous work in dairy cows examining increases in milk yield due to increased milking frequency have identified changes in apoptosis and expression of genes regulating cytokine signaling. In addition, changes in mitochondrial biogenesis and function have been suggested to play a role during the lactation cycle in regulating milk production. The goal of this study was to test the hypothesis that, when maintained over an entire lactation, extreme differences in milking frequency would be reflected in differences in apoptosis, mammary mitochondrial number, and the mammary expression of genes known to inhibit cytokine signaling. Primiparous Holstein cows (n=6) were assigned to the study 40d before parturition after which 1 udder half was milked once daily (1×) and the other 4 times daily (4×) Mammary biopsies were collected at 15, 60, 120, and 230d of lactation. Average milk yield from the 4× side was 3 times higher than from the 1× side. Analysis of milk composition revealed that protein, lactose, and solids-not-fat percentages were lower in 1× than 4× udder halves. Mammary cell apoptosis was not affected by milking frequency. Mammary cell mitochondrial number, as estimated by succinate dehydrogenase staining, was higher in early lactation, decreasing as days in milk increased, and with increased milking frequency. Although mammary expression of α-lactalbumin (LALBA) and β-casein (CSN2) was significantly increased in 4× glands, the expression of suppressors of cytokine signaling were similar between 1×- and 4×-milked halves. These results support the conclusion that changes in milk production in response to extreme differences in milking frequency may be related to alterations in mitochondrial number and lactose synthesis, but not apoptosis.

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“…G. candidum lipase was reported to be cis-9and cis,cis-9,12-fatty acid specific, and these acids were hydrolyzed regardless of their triacylglycerol positions (32). Staphylococcus aureus lipolytic activity plays an important role in pathogenesis resulting in significant increases of oleic acid in plasma (13,50) and consequently causing an inhibition of protein-oxidative phosphorylation (9,29). S. aureus lipase was nonspecific in hydrolyzing the acyl-glycerol bond regardless of either the position or fatty acid involved (47).…”

Section: Discussionmentioning

confidence: 99%

Microbiological transformations of lipids: acyl-specific hydrolysis of lard by yeasts

El-Sharkawy

Dostal

Rosazza

1993

Appl Environ Microbiol

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The fatty acid and positional specificities of Saccharomyces cerevisiae (UI-SACCH) and Schizosaccharomyces octosporus (NRRL Y-854) in the hydrolysis of lard were studied by using gas-liquid chromatography. Synthetic triglycerides were used to determine the positional specificities of the lipases of both organisms. Palmitic acid is specifically cleaved from all three triglyceride ester positions by S. cerevisiae, while S. octosporus was able to cleave stearic acid at either position 1 or position 3 of the glycerol moiety. Preparative scale fermentation with 200 g of lard per liter yielded 48.4 g of palmitic acid per liter with S. cerevisiae and 42 g of stearic acid per liter with S. octosporus. The free fatty acids produced by microbial transformation of lard were characterized spectrally ('H and C nuclear magnetic resonance and mass spectrometry) and chromatographically (thin-layer and gas chromatographies).

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Oxidative phosphorylation in guinea pig mammary gland mitochondria during various functional states

Cited by 19 publications

References 11 publications

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Milk yield differences between 1× and 4× milking are associated with changes in mammary mitochondrial number and milk protein gene expression, but not mammary cell apoptosis or SOCS gene expression

Microbiological transformations of lipids: acyl-specific hydrolysis of lard by yeasts

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