Oxidative phosphorylation in bone cells

Sabini, Elena; Arboit, Lorenzo; Khan, Mohd Parvez; Lanzolla, Giulia; Schipani, Ernestina

doi:10.1016/j.bonr.2023.101688

Cited by 7 publications

(4 citation statements)

References 60 publications

(80 reference statements)

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“…In an effort to further explore the cause in the aforementioned results, we evaluated key molecules of oxidative stress and energy metabolism, cellular processes that affect bone health and bone-related diseases such as osteoporosis [16,[49][50][51][52][53][54]. We first examined the gene and/or protein expression levels of Pgc1a and Tfam, as important factors of mitochondrial biogenesis in bone cells [24].…”

Section: Discussionmentioning

confidence: 99%

Defining the most potent osteoinductive culture conditions for MC3T3-E1 cells reveals no implication of oxidative stress or energy metabolism

Semicheva,

Ersoy,

Vasilaki

et al. 2024

Preprint

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MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge and, particularly, the osteogenic medium (OSM) composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regards to mineralization capacity and explore if oxidative stress or cellular metabolism processes are implicated. Cells were treated with 9 different combinations of long-lasting ascorbate (Asc) and beta-glycerophosphate (betaGP) and osteogenesis/calcification were evaluated at 3 different time-points by qPCR, Western blotting and bone nodule staining. Key molecules of oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in 150 ug.mL-1/2mM Asc/betaGP combination at 21d in OSM and this was supported by Runx2, Alpl, Bglap and Col1a1 expression levels increase. NOX2 and SOD2 as well as PGC1alpha and Tfam were also monitored as indicators of redox and metabolic processes, respectively, where no differences were observed. Elevation in OCN protein levels and ALP activity showed that mineralisation comes as a result of these differences. This work defines the most appropriate culture conditions for MC3T3-E1 cells and could be used by other research laboratories in this field.

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Section: Discussionmentioning

confidence: 99%

Defining the most potent osteoinductive culture conditions for MC3T3-E1 cells reveals no implication of oxidative stress or energy metabolism

Semicheva,

Ersoy,

Vasilaki

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In an effort to further explore the cause of the aforementioned results, we evaluated key molecules of oxidative stress and the energy metabolism, cellular processes which affect bone health and bone-related diseases such as osteoporosis [16,[69][70][71][72][73][74]. We first examined the gene and/or protein expression levels of Pgc1a and Tfam, important factors of mitochondrial biogenesis in bone cells [24].…”

Section: Discussionmentioning

confidence: 99%

Defining the Most Potent Osteoinductive Culture Conditions for MC3T3-E1 Cells Reveals No Implication of Oxidative Stress or Energy Metabolism

Semicheva,

Ersoy,

Vasilaki

et al. 2024

IJMS

View full text Add to dashboard Cite

The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)’s composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regard to their mineralization capacity and explore if oxidative stress or the cellular metabolism processes are implicated. Cells were treated with nine different combinations of long-lasting ascorbate (Asc) and β-glycerophosphate (βGP), and osteogenesis/calcification was evaluated at three different time-points by qPCR, Western blotting, and bone nodule staining. Key molecules of the oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in the 150 μg.mL−1/2 mM Asc/βGP combination on day 21 in OSM, and this was supported by Runx2, Alpl, Bglap, and Col1a1 expression level increases. NOX2 and SOD2 as well as PGC1α and Tfam were also monitored as indicators of redox and metabolic processes, respectively, where no differences were observed. Elevation in OCN protein levels and ALP activity showed that mineralisation comes as a result of these differences. This work defines the most appropriate culture conditions for MC3T3-E1 cells and could be used by other research laboratories in this field.

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Section: An Overview Of Immunometabolites In Viral Infectionsmentioning

confidence: 99%

“…OXPHOS is a crucial metabolic pathway in eukaryotic cells' mitochondria 196 . It involves the generation of ATP through the transfer of electrons along the ETC coupled with the phosphorylation of ADP to ATP 196 . OXPHOS plays a central role in cellular energy production and is essential for various cellular processes, including growth, differentiation, and immune function 197,198 .…”

Section: An Overview Of Immunometabolites In Viral Infectionsmentioning

confidence: 99%

Immunometabolites in viral infections: Action mechanism and function

Bouzari,

Chugaeva,

Karampoor

et al. 2024

Journal of Medical Virology

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The interplay between viral pathogens and host metabolism plays a pivotal role in determining the outcome of viral infections. Upon viral detection, the metabolic landscape of the host cell undergoes significant changes, shifting from oxidative respiration via the tricarboxylic acid (TCA) cycle to increased aerobic glycolysis. This metabolic shift is accompanied by elevated nutrient accessibility, which is vital for cell function, development, and proliferation. Furthermore, depositing metabolites derived from fatty acids, TCA intermediates, and amino acid catabolism accelerates the immunometabolic transition, facilitating pro‐inflammatory and antimicrobial responses. Immunometabolites refer to small molecules involved in cellular metabolism regulating the immune response. These molecules include nutrients, such as glucose and amino acids, along with metabolic intermediates and signaling molecules adenosine, lactate, itaconate, succinate, kynurenine, and prostaglandins. Emerging evidence suggests that immunometabolites released by immune cells establish a complex interaction network within local niches, orchestrating and fine‐tuning immune responses during viral diseases. However, our current understanding of the immense capacity of metabolites to convey essential cell signals from one cell to another or within cellular compartments remains incomplete. Unraveling these complexities would be crucial for harnessing the potential of immunometabolites in therapeutic interventions. In this review, we discuss specific immunometabolites and their mechanisms of action in viral infections, emphasizing recent findings and future directions in this rapidly evolving field.

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Oxidative phosphorylation in bone cells

Cited by 7 publications

References 60 publications

Defining the most potent osteoinductive culture conditions for MC3T3-E1 cells reveals no implication of oxidative stress or energy metabolism

Defining the most potent osteoinductive culture conditions for MC3T3-E1 cells reveals no implication of oxidative stress or energy metabolism

Defining the Most Potent Osteoinductive Culture Conditions for MC3T3-E1 Cells Reveals No Implication of Oxidative Stress or Energy Metabolism

Immunometabolites in viral infections: Action mechanism and function

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