Oxidative Modifications of Rat Liver Cell Components During<i>Fasciola hepatica</i>Infection

Siemieniuk, Ewa; Kołodziejczyk, Lidia; Skrzydlewska, Elżbieta

doi:10.1080/15376510701624001

Cited by 19 publications

(16 citation statements)

References 42 publications

(47 reference statements)

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“…These pathological processes can be simultaneously observed in some diseases, such as diabetes, cadmium poisoning, and neurodegenerative diseases (24,30,45). Additionally, there is growing evidence that oxidative stress plays an important role in infection-induced apoptosis (11,31,41).…”

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confidence: 99%

Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice

Liu

Zhang

et al. 2012

Infect Immun

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dToxoplasma gondii infection in pregnant women may result in abortion or in fetal teratogenesis; however, the underlying mechanisms are still unclear. In this paper, based on a murine model, we showed that maternal infection with RH strain T. gondii tachyzoites induced elevated production of reactive oxygen species (ROS), local oxidative stress, and subsequent apoptosis of placental trophoblasts. PCR array analysis of 84 oxidative stress-related genes demonstrated that 27 genes were upregulated at least 2-fold and that 9 genes were downregulated at least 2-fold in the T. gondii infection group compared with levels in the control group. The expression of NADPH oxidase 1 (Nox1) and glutathione peroxidase 6 (Gpx6) increased significantly, about 25-fold. The levels of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) increased significantly with T. gondii infection, and levels of glutathione (GSH) decreased rapidly. T. gondii infection increased the early expression of endoplasmic reticulum stress (ERS) markers, followed by cleavage of caspase-12, activation of ASK1/JNK, and increased apoptosis of trophoblasts, both in vivo and in vitro. The apoptosis of trophoblasts, the activation of caspase-12 and the ASK1/JNK pathway, and the production of peroxides were dramatically inhibited by pretreatment with N-acetylcysteine (NAC). The upregulation of Nox1 was contact dependent and preceded the increase in levels of ERS markers and the activation of the proapoptosis cascade. Thus, we concluded that apoptosis in placental trophoblasts was initiated predominantly by ROS-mediated ERS via activation of caspase-12, CHOP, and the JNK pathway in acute T. gondii infection. Elevated ROS production is the central event in T. gondiiinduced apoptosis of placental trophoblasts.

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confidence: 99%

Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice

Liu

Zhang

et al. 2012

Infect Immun

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“…Beside dismutation, the Cu/Zn SOD izoenzyme catalyzes surrogate reactions, such as the production of hydroxyl radicals, using anionic scavengers and hydrogen peroxide [41]. The very reactive hydroxyl radicals react rapidly with a wide range of biological macromolecules, causing oxidative damage [42] and a decrease in activity/level of basic cellular enzymatic and non-enzymatic antioxidants [43]. Recent studies proved that in CCl 4 exposed mice the level of SOD, CAT and GPX mRNAs decreased significantly [44].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

et al. 2015

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“…Glaucomatous eyes are often characterized with a high level of oxidative stress that can lead to cell death 9 10 . Given that GNGA materials exhibit excellent free radical scavenging abilities due to grafting of GA molecules, in vitro oxidative challenges are employed to evaluate their antioxidant capacities.…”

Section: Resultsmentioning

confidence: 99%

In vivo Pharmacological Evaluations of Pilocarpine-Loaded Antioxidant-Functionalized Biodegradable Thermogels in Glaucomatous Rabbits

Chou

Luo

Lai

2017

Sci Rep

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To alleviate oxidative stress-induced ocular hypertension, grafting of antioxidant molecules to drug carriers enables a dual-function mechanism to effectively treat glaucomatous intraocular pressure (IOP) dysregulation. Providing potential application for intracameral administration of antiglaucoma medications, this study, for the first time, aims to examine in vivo pharmacological efficacy of pilocarpine-loaded antioxidant-functionalized biodegradable thermogels in glaucomatous rabbits. A series of gallic acid (GA)-grafted gelatin-g-poly(N-isopropylacrylamide) (GN) polymers were synthesized via redox reactions at 20–50 °C. Our results showed that raising redox radical initiation reaction temperature maximizes GA grafting level, antioxidant activity, and water content at 40 °C. Meanwhile, increase in overall hydrophilicity of GNGA carriers leads to fast polymer degradation and early pilocarpine depletion in vivo, which is disadvantageous to offer necessary pharmacological performance at prolonged time. By contrast, sustained therapeutic drug concentrations in aqueous humor can be achieved for long-term (i.e., 28 days) protection against corneal aberration and retinal injury after pilocarpine delivery using dual-function optimized carriers synthesized at 30 °C. The GA-functionalized injectable hydrogels are also found to contribute significantly to enhancement of retinal antioxidant defense system and preservation of histological structure and electrophysiological function, thereby supporting the benefits of drug-containing antioxidant biodegradable thermogels to prevent glaucoma development.

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Oxidative Modifications of Rat Liver Cell Components DuringFasciola hepaticaInfection

Cited by 19 publications

References 42 publications

Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice

Reactive Oxygen Species-Triggered Trophoblast Apoptosis Is Initiated by Endoplasmic Reticulum Stress via Activation of Caspase-12, CHOP, and the JNK Pathway in Toxoplasma gondii Infection in Mice

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

In vivo Pharmacological Evaluations of Pilocarpine-Loaded Antioxidant-Functionalized Biodegradable Thermogels in Glaucomatous Rabbits

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