Oxidative Lipidomics: Analysis of Oxidized Lipids and Lipid Peroxidation in Biological Systems with Relevance to Health and Disease

Kodali, Surya T.; Kauffman, Philip; Kotha, Sainath R.; Yenigalla, Anita; Veeraraghavan, Rengasayee; Pannu, Sonal; Hund, Thomas J.; Satoskar, Abhay R.; McDaniel, Jodi C.; Maddipati, Rao K.; Parinandi, Narasimham L.

doi:10.1007/978-3-030-47318-1_5

Cited by 13 publications

(8 citation statements)

References 72 publications

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“…The proportional depletions in omega-3 and omega-6 polyunsaturated FAs due to multi-mycotoxin exposure were accompanied by notable decreases in unsaturation index and average chain length. These changes affect the sensitivity of lipids to oxidation and lipid peroxidation that occur as a result of ROS [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Combined Exposure of Fusarium Mycotoxins on Lipid Peroxidation, Antioxidant Defense, Fatty Acid Profile, and Histopathology in Laying Hens’ Liver

Kulcsár,

Turbók,

Kövér

et al. 2024

Toxins

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Fumonisin B1, T-2 toxin, and deoxynivalenol are frequently detected in feed materials. The mycotoxins induce free radical formation and, thereby, lipid peroxidation. The effects of mycotoxin exposure at the EU recommended limit (T-2/HT-2 toxin: 0.25 mg/kg; DON = 3AcDON/15-AScDON: 5 mg/kg; fumonisin B1: 20 mg/kg) and double dose (T-2/HT-2 toxin: 0.5 mg/kg, DON/3-AcDON/15-AcDON: 10 mg, and FB1: 40 mg/kg feed) were investigated during short-term (3 days) per os exposure in the liver of laying hens. On day 1 higher while on day 3 lower MDA concentrations were found in the low-dose group compared to the control. Fatty acid composition also changed: the proportion of monounsaturated fatty acids increased (p < 0.05) and the proportion of polyunsaturated fatty acids decreased by day 3. These alterations resulted in a decrease in the index of unsaturation and average fatty acid chain length. Histopathological alterations suggested that the incidence and severity of liver lesions were higher in the mycotoxin-treated laying hens, and the symptoms correlated with the fatty acid profile of total phospholipids. Overall, the findings revealed that mycotoxin exposure, even at the EU-recommended limits, induced lipid peroxidation in the liver, which led to changes in fatty acid composition, matched with tissue damage.

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Section: Discussionmentioning

confidence: 99%

The Effect of Combined Exposure of Fusarium Mycotoxins on Lipid Peroxidation, Antioxidant Defense, Fatty Acid Profile, and Histopathology in Laying Hens’ Liver

Kulcsár,

Turbók,

Kövér

et al. 2024

Toxins

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“…These fluorescent compounds (FCs) have not been fully chemically defined but are widely used as markers of lipid oxidation (Ivica and Wilhelm, 2014). In addition, when polyunsaturated fatty acids are oxidized, rearrangements of double bonds lead to the formation of membrane conjugated dienes (CDs) that are also a marker of lipid peroxidation (Kodali et al, 2020). To determine the production of FCs and CDs we conducted measurements on synaptosomal membranes, which were extracted using 2:1 chloroform: methanol containing 0.005% Beta-hydroxytoluene and 0.5 mM EDTA according to the method of Folch et al (Folch et al, 1957).…”

Section: Biochemical Analysismentioning

confidence: 99%

Hypercapnia Causes Injury of the Cerebral Cortex and Cognitive Deficits in Newborn Piglets

Fritz,

Sanidas,

Cardenas

et al. 2024

eNeuro

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In critically ill newborns, exposure to hypercapnia (HC) is common and often accepted in neonatal intensive care units (NICUs) to prevent severe lung injury. However, as a “safe” range of arterial partial pressure of carbon dioxide (PaCO2) levels in neonates has not been established, the potential impact of HC on the neurodevelopmental outcomes in these newborns remains a matter of concern. Here, in a newborn Yorkshire piglet model of either sex, we show that acute exposure to HC induced persistent cortical neuronal injury, associated cognitive and learning deficits, and long-term suppression of cortical electroencephalographic (EEG) frequencies. HC induced a transient energy failure in cortical neurons, a persistent dysregulation of calcium-dependent pro-apoptotic signaling in the cerebral cortex, and activation of the apoptotic cascade, leading to nuclear deoxyribonucleic acid (DNA) fragmentation. While neither one hour of HC nor the rapid normalization of HC were associated with changes in cortical bioenergetics, rapid resuscitation resulted in a delayed onset of synaptosomal membrane lipid peroxidation, suggesting a dissociation between energy failure and the occurrence of synaptosomal lipid peroxidation. Even short durations of HC triggered biochemical responses at the subcellular level of the cortical neurons resulting in altered cortical activity and impaired neurobehavior. The deleterious effects of HC on the developing brain should be carefully considered as crucial elements of clinical decisions in the neonatal intensive care unit.Significance StatementHospitalized critically ill neonates commonly experience hypercapnia as part of common practice in neonatal intensive care, as ventilated newborns are subjected to "permissive hypercapnia" to reduce lung injury associated with positive pressure ventilation. To answer the clinically important question of how "safe" that practice is, the present study provides new insights into the complex effects of hypercapnia on neuronal processes, with significant ramifications for ongoing neurodevelopment.

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“…In this sense, antioxidants act in the neutralization of free radicals, donating hydrogen molecules and stabilizing them. On the other hand, some antioxidants act by inhibiting prooxidant agents, delaying lipid oxidation (Kodali et al, 2020).…”

Section: Lipid Oxidation In Meatmentioning

confidence: 99%

Use of ultrasound and acerola (Malpighia emarginata) residue extract in meat pork

et al. 2023

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Consumers are more demanding in relation to meat quality, developing new demands, such as increased shelf life and good sensory quality. The tenderness of the meat is one of the most important elements in the choice of the product, the modification in the intrinsic structure of the meat, for example, increase of the proteolysis and fragmentation of the myofibrils contribute to the improvement of the tenderness. However, it is necessary to develop technological strategies so that this ultrasound technology can be added to improve meat tenderness. Thus, the objective of this review was to know the main aspects of the use of ultrasound and acerola residue in meat tenderness. Overall, existing research demonstrates excellent prospects for this new redesign approach.

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Oxidative Lipidomics: Analysis of Oxidized Lipids and Lipid Peroxidation in Biological Systems with Relevance to Health and Disease

Cited by 13 publications

References 72 publications

The Effect of Combined Exposure of Fusarium Mycotoxins on Lipid Peroxidation, Antioxidant Defense, Fatty Acid Profile, and Histopathology in Laying Hens’ Liver

The Effect of Combined Exposure of Fusarium Mycotoxins on Lipid Peroxidation, Antioxidant Defense, Fatty Acid Profile, and Histopathology in Laying Hens’ Liver

Hypercapnia Causes Injury of the Cerebral Cortex and Cognitive Deficits in Newborn Piglets

Use of ultrasound and acerola (Malpighia emarginata) residue extract in meat pork

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