Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox

Štrosová, Miriam; Karlovska, Jana; Spickett, Corinne M.; Grune, Tilman; Orszagova, Zuzana; Horáková, Ľubica

doi:10.4149/gpb_2009_02_195

Cited by 11 publications

(3 citation statements)

References 69 publications

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“…Sarco/ER Ca 2+ ‐ATPase (SERCA) is another important Ca 2+ pumps, which is a Ca 2+ ATPase which transfers Ca 2+ from the cytosol of the cell to ER at the expense of ATP hydrolysis. It has been reported that H 2 O 2 can inhibit Ca 2+ re‐uptake by SERCA [Redondo et al, 2004; Voss et al, 2008; Strosova et al, 2009]. Withstanding the experiment limitations, our data do not imply SERCA.…”

Section: Discussioncontrasting

confidence: 44%

Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells

Wei

Dai

et al. 2010

J of Cellular Biochemistry

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Wogonin, a major active constituent of Scutellaria baicalensis, possesses potent anticancer activities both in vivo and in vitro. This paper describes the different apoptotic effects of wogonin in HepG2 and L02 cells and the possible mechanism for the differences. Through DAPI staining, Annexin-V/PI double-staining assay, JC-1 detection and the expressions of the key apoptotic proteins, we find that wogonin prefers to induce apoptosis in HepG2 cells through the mitochondrial pathway, while has much less effects on L02 cells. Moreover, overexpression of Bcl-2 can block wogonin-induced apoptosis in HepG2 cells. To illustrate the specific selective mechanism of wogonin in apoptosis induction, H(2)O(2), (·)O(2)(-) and Ca(2+) are measured by 2',7'-dichlorfluorescein-diacetate, dihydroethidium and Flou-3 AM assay, respectively. The results show that the different apoptotic effects of wogonin in HepG2 and L02 cells are due to the different regulations to the redox balance of reactive oxygen species and the Ca(2+) release from endoplasmic reticulum. IP(3)R-sensitive Ca(2+) channels are the key targets of the wogonin-increased H(2)O(2). Besides, the activation of PLCγ1 plays as a bridge between H(2)O(2) signal molecules and Ca(2+) release. Taken together, wogonin preferentially kills hepatoma cells by H(2)O(2)-dependent apoptosis triggered by Ca(2+) overload. The results reveal that wogonin is a competitive anticancer drug candidate for the malignant hepatoma therapy.

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Section: Discussioncontrasting

confidence: 44%

Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells

Wei

Dai

et al. 2010

J of Cellular Biochemistry

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“…Trp emission spectra were collected by exciting the sample at 290 nm and the emission spectra were recorded in the range of 300-450 nm (Carney et al 2007). The intensity ratio I 336 nm /I 358 nm established previously by Restall et al (1986), as a determinant of SERCA1 conformational changes was evaluated according to (Strosova et al 2009). …”

Section: Intrinsic Tryptophan Fluorescencementioning

confidence: 99%

Inhibition of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA1) by rutin derivatives

Viskupičová

Májeková

Horáková

2014

J Muscle Res Cell Motil

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The effect of lipophilic rutin derivatives (acylated with fatty acid chain length of 16-22) on sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA1 isoform) compared to the parent molecule rutin was evaluated. Rutin derivatives caused concentration dependent decrease of SERCA1 activity (IC50 ~ 23-64 µM) and significant conformational alterations in the transmembrane region of the enzyme. Upon treatment by peroxynitrite, rutin derivatives exerted a hormetic effect, i.e. prevented enzyme activity decrease at low concentrations, while additionally inhibited at high concentrations. Concerning the posttranslational modifications of SERCA1, rutin esters: (i) induced a significant loss of free sulfhydryl groups, (ii) protected the enzyme from protein carbonyl formation, and (iii) prevented SERCA from tyrosine nitration (except R20:4 and R22:1). In silico study revealed a strong affinity of the derivative R20:4 to the transmembrane region of SERCA1, stabilized via hydrogen bonds with Glu90, Glu771, Thr778 and Thr848 residues. Interaction of rutin derivatives with Glu771, a residue involved in Ca(2+) binding, is likely to be responsible for the inhibitory effect of the esters.

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“…Studies suggest that Cl 2 inhalation damages both airway and alveolar epithelial tissues and that these damaging effects were ameliorated by the prophylactic administration of low molecular-weight antioxidants. Trolox was reported to be protective against oxidative injury induced by HOCl to Ca-ATPase in the sarcoplasmic reticulum of skeletal muscle [220]. Ascorbic acid might also play a protective role (Table 4), especially in individuals consuming supplements containing this vitamin.…”

Section: Purposementioning

confidence: 99%

The Protective Role of Antioxidants in the Defence against ROS/RNS-Mediated Environmental Pollution

Poljšak

Fink

2014

Oxidative Medicine and Cellular Longevity

186

104

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Overproduction of reactive oxygen and nitrogen species can result from exposure to environmental pollutants, such as ionising and nonionising radiation, ultraviolet radiation, elevated concentrations of ozone, nitrogen oxides, sulphur dioxide, cigarette smoke, asbestos, particulate matter, pesticides, dioxins and furans, polycyclic aromatic hydrocarbons, and many other compounds present in the environment. It appears that increased oxidative/nitrosative stress is often neglected mechanism by which environmental pollutants affect human health. Oxidation of and oxidative damage to cellular components and biomolecules have been suggested to be involved in the aetiology of several chronic diseases, including cancer, cardiovascular disease, cataracts, age-related macular degeneration, and aging. Several studies have demonstrated that the human body can alleviate oxidative stress using exogenous antioxidants. However, not all dietary antioxidant supplements display protective effects, for example, β-carotene for lung cancer prevention in smokers or tocopherols for photooxidative stress. In this review, we explore the increases in oxidative stress caused by exposure to environmental pollutants and the protective effects of antioxidants.

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Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox

Cited by 11 publications

References 69 publications

Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells

Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells

Inhibition of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA1) by rutin derivatives

The Protective Role of Antioxidants in the Defence against ROS/RNS-Mediated Environmental Pollution

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Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox

Cited by 11 publications

References 69 publications

Different apoptotic effects of wogonin via induction of H2O2 generation and Ca2+ overload in malignant hepatoma and normal hepatic cells

Different apoptotic effects of wogonin via induction of H2O2 generation and Ca2+ overload in malignant hepatoma and normal hepatic cells

Inhibition of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA1) by rutin derivatives

The Protective Role of Antioxidants in the Defence against ROS/RNS-Mediated Environmental Pollution

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Product

Resources

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Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells

Different apoptotic effects of wogonin via induction of H₂O₂ generation and Ca²⁺ overload in malignant hepatoma and normal hepatic cells