Oxidative injury induced by drinking water disinfection by-products dibromoacetonitrile and dichloroacetonitrile in mouse hippocampal neuronal cells: The protective effect of N-acetyl-L-cysteine

Li, Fang; Zhou, Jie; Zhu, Xueyu; Lu, Rongzhu; Yang, Yong; Wang, Suhua; Xing, Guangwei; Shen, Honglie

doi:10.1016/j.toxlet.2022.06.005

Cited by 9 publications

(2 citation statements)

References 44 publications

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“…When compared to acetonitrile, CAN is 10 times more hazardous. CANs are highly hazardous nitrogenous disinfection by‐products (N‐DBPs) that are commonly found in water supply systems and have gotten a lot of attention (Li et al, 2022). It's been employed as a fumigant and is an organic intermediary in the production of fenoxycarb, an insecticide, and guanethidine, a cardiovascular medication.…”

Section: Introductionmentioning

confidence: 99%

Chloroacetonitrile reduces rat prenatal bone length and induces oxidative stress, apoptosis, and DNA damage in rat fetal liver

Abd-Allah

Fouad

Ghareeb

et al. 2023

Birth Defects Research

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One of the disinfection byproducts of chlorinating drinking water is chloroacetonitrile (CAN). Thirty-six female rats were used and distributed equally into four groups. The low dose treated group received CAN at a dose of 5.5 mg/kg body weight/day (1/40 LD 50 ) orally from the 6th to 12th day of gestation. The high dose treated group received 11 mg/kg body weight/day (1/20 LD 50 ) of CAN orally for the same period, the vehicle control group received 1 mL of corn oil, and the water control group received 1 mL of distilled water orally for the same period. High dose exposure to CAN significantly reduced gravid uterine weight, fetal body weights, and length, and caused obvious skeletal deformities, weak mineralization. Fetal tibial growth plates displayed histopathologic changes. Induced oxidative stress and redox imbalance in fetal liver tissues was evidenced by significantly decreased in catalase and superoxide dismutase activity, and elevated malondialdehyde levels. Histopathological, glycogen content changes, and DNA damage were observed in the fetal liver of high dose treated group. Additionally, administration of high dose of CAN induced apoptosis, evidenced by increased caspase-3 concentration in fetal liver. Thus, extensive exposure to CAN induces poor pregnancy outcomes.CAN levels in water should be monitored regularly. K E Y W O R D S chloroacetonitrile, DNA damage, fetal liver, rat, water disinfection byproducts Highlights • Pregnant rats were given chloroacetonitrile (CAN) orally during organogenesis. • Extensive exposure to CAN caused a reduction in lengths of fetal bones and damage to fetal hepatic tissues. • High dose maternally treated fetuses showed elevated levels of caspase-3 activity and DNA damage.

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Section: Introductionmentioning

confidence: 99%

Chloroacetonitrile reduces rat prenatal bone length and induces oxidative stress, apoptosis, and DNA damage in rat fetal liver

Abd-Allah

Fouad

Ghareeb

et al. 2023

Birth Defects Research

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“…DBPs have been reported to be neurotoxic [ 22 ] and activate the Nrf2-mediated oxidative stress response pathway [ 23 ] by reducing intracellular glutathione and increasing ROS [ 24 ]. Further there is some but not strong evidence that DBP exposure may adversely affect neuropsychological development [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water

Escher

Blanco

Caixach

et al. 2023

J Expo Sci Environ Epidemiol

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Background Location-specific patterns of regulated and non-regulated disinfection byproducts (DBPs) were detected in tap water samples of the Barcelona Metropolitan Area. However, it remains unclear if the detected DBPs together with undetected DPBs and organic micropollutants can lead to mixture effects in drinking water. Objective To evaluate the neurotoxicity, oxidative stress response and cytotoxicity of 42 tap water samples, 6 treated with activated carbon filters, 5 with reverse osmosis and 9 bottled waters. To compare the measured effects of the extracts with the mixture effects predicted from the detected concentrations and the relative effect potencies of the detected DBPs using the mixture model of concentration addition. Methods Mixtures of organic chemicals in water samples were enriched by solid phase extraction and tested for cytotoxicity and neurite outgrowth inhibition in the neuronal cell line SH-SY5Y and for cytotoxicity and oxidative stress response in the AREc32 assay. Results Unenriched water did not trigger neurotoxicity or cytotoxicity. After up to 500-fold enrichment, few extracts showed cytotoxicity. Disinfected water showed low neurotoxicity at 20- to 300-fold enrichment and oxidative stress response at 8- to 140-fold enrichment. Non-regulated non-volatile DBPs, particularly (brominated) haloacetonitriles dominated the predicted mixture effects of the detected chemicals and predicted effects agreed with the measured effects. By hierarchical clustering we identified strong geographical patterns in the types of DPBs and their association with effects. Activated carbon filters did not show a consistent reduction of effects but domestic reverse osmosis filters decreased the effect to that of bottled water. Impact statement Bioassays are an important complement to chemical analysis of disinfection by-products (DBPs) in drinking water. Comparison of the measured oxidative stress response and mixture effects predicted from the detected chemicals and their relative effect potencies allowed the identification of the forcing agents for the mixture effects, which differed by location but were mainly non-regulated DBPs. This study demonstrates the relevance of non-regulated DBPs from a toxicological perspective. In vitro bioassays, in particular reporter gene assays for oxidative stress response that integrate different reactive toxicity pathways including genotoxicity, may therefore serve as sum parameters for drinking water quality assessment.

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Toxicity of DBPs

Du,

2024

The Handbook of Environmental Chemistry

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Oxidative injury induced by drinking water disinfection by-products dibromoacetonitrile and dichloroacetonitrile in mouse hippocampal neuronal cells: The protective effect of N-acetyl-L-cysteine

Cited by 9 publications

References 44 publications

Chloroacetonitrile reduces rat prenatal bone length and induces oxidative stress, apoptosis, and DNA damage in rat fetal liver

Chloroacetonitrile reduces rat prenatal bone length and induces oxidative stress, apoptosis, and DNA damage in rat fetal liver

In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water

Toxicity of DBPs

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