Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration

Abstract

Plant derived flavonoids have not been well explored in tissue engineering applications due to difficulties in efficient formulations with biomaterials for controlled presentation. Here, the authors report that surface coating of epigallocatechin gallate (EGCG) on polymeric substrates including poly (L‐lactic acid) (PLLA) nanofibers can be performed via oxidative polymerization of EGCG in the presence of cations, enabling regulation of biological functions of multiple cell types implicated in bone regeneration. EGCG coating on the PLLA nanofiber promotes osteogenic differentiation of adipose‐derived stem cells (ADSCs) and is potent to suppress adipogenesis of ADSCs while significantly reduces osteoclastic maturation of murine macrophages. Moreover, EGCG coating serves as a protective layer for ADSCs against oxidative stress caused by hydrogen peroxide. Finally, the in vivo implantation of EGCG‐coated nanofibers into a mouse calvarial defect model significantly promotes the bone regeneration (61.52 ± 28.10%) as compared to defect (17.48 ± 11.07%). Collectively, the results suggest that EGCG coating is a simple bioinspired surface modification of polymeric biomaterials and importantly can thus serve as a promising interface for tuning activities of multiple cell types associated with bone fracture healing.

Utility of Thermal Cross-Linking in Stabilizing Hydrogels with Beta-Tricalcium Phosphate and/or Epigallocatechin Gallate for Use in Bone Regeneration Therapy

“…In this study, vhEc-GS-β containing EGCG induced more rapid resorption of the encapsulated β-TCP granules than did β-TCP alone and more osteoclasts, at least in the condition using 28 mg β-TCP. This discrepancy may be due to the different doses of EGCG; EGCG inhibits osteoclast differentiation at >20 μM [ 15 , 45 ], possibly via inhibiting the receptor activator of NF-κB ligand (RANKL) [ 46 ], whereas it promotes osteoblast differentiation, such as stem cells, from 1 to 10 μM [ 47 , 48 ]. The dose of EGCG in vhEc-GS was established based on the concentrations that significantly promote osteogenesis from previous studies [ 26 ].…”

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

“…The results show that PLLA nanofiber does not perform well in promoting the proliferation of bone marrow mesenchymal cells, but also inhabiting the activity of RAW2.64.7 osteoclasts. Moreover, the scaffold coating EGCG can provide a proper environment for osteoblast (Madhurakkat Perikamana et al, 2019). Compared with the scaffold, not coating EGCG, nanofiber coating EGCG significantly suppress inflammation.…”

“…Nevertheless, chemical approaches coupling nanoparticles and EGCG may increase potential cytotoxicity. Some reports put out the view of EGCG coating, a form of easy physical adsorption to probably take the place of chemical approach and reduce the cytotoxicity further (Madhurakkat Perikamana et al, 2019).…”

Assessing the analgesic efficacy of oral epigallocatechin-3-gallate on epidural catheter analgesia in patients after surgical stabilisation of multiple rib fractures: a prospective double-blind, placebo-controlled clinical trial

“…Oxidative EGCG coating on polymeric substrates has been demonstrated to regulate activities of multiple cell types that are beneficial for healing of bone fractures (Madhurakkat Perikamana et al. 2019 ). However, the role of EGCG in pain management of MRF has yet to be investigated.…”