Oxidative effects of the acute exposure to a pesticide mixture of cypermethrin and chlorpyrifos on carp and zebrafish – A comparative study

Nunes, Mauro Eugênio Medina; Müller, Talise E.; Murussi, Camila; Amaral, Aline Monique Blank do; Gomes, Jeane L.C.; Marins, Aline Teixeira; Leitemperger, Jossiele; Rodrigues, Cíntia Corte Real; Fiuza, Tiago da Luz; Costa, Maiara; Severo, Eduardo Stringini; Loro, Vania Lúcia

doi:10.1016/j.cbpc.2018.03.002

Cited by 35 publications

(22 citation statements)

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“…Moreover, we identified 12 transcripts in cluster 5 as GST genes; one of these was uniquely expressed in larvae feeding on N. tabacum . This GST gene is a member of the microsomal subfamily that is thought to be involved in oxidative stress response [64, 65].…”

Section: Discussionmentioning

confidence: 99%

An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)

2019

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BackgroundTo understand the genetic mechanisms of insect herbivory, the transcriptional response of insects feeding on different host plant species has to be studied. Here, we generated gene expression data of the generalist herbivore Spodoptera exigua (Hübner) feeding on three selected host plant species and a control (artificial diet). The host plant species used in this study –cabbage (Brassica oleracea), maize (Zea mays) and tobacco (Nicotiana tabacum)- are members of different plant families that each employ specific defence mechanisms and toxins.ResultsSpodoptera exigua larvae had a higher growth rate, indicator for herbivore success, when feeding on Z. mays compared to larvae feeding on B. oleracea or N. tabacum. Larvae feeding on the different host plant species showed divergent transcriptional responses. We identified shared and unique gene expression patterns dependent of the host plant species the larvae fed on. Unique gene expression patterns, containing uniquely upregulated transcripts including specific detoxification genes, were found for larvae feeding on either B. oleracea or N. tabacum. No diet-specific gene cluster was identified for larvae feeding on the host for which larvae showed optimal herbivore success, Z. mays, or artificial diet. In contrast, for larvae feeding on hosts for which they showed low herbivore success, specific diet-dependent gene clusters were identified. Functional annotation of these clusters indicates that S. exigua larvae deploy particular host plant-specific genes for digestion and detoxification.ConclusionsThe lack of a host plant-specific gene activity for larvae feeding on Z. mays and the artificial diet suggest a general and non-specific gene activity for host plants with optimal herbivore success. Whereas the finding of specific gene clusters containing particular digestion and detoxifying genes expressed in larvae feeding on B. oleracea and N. tabacum, with low herbivore success, imply a host plant-specific gene activity for larvae feeding on host plants with suboptimal herbivore success. This observation leads to the conclusion that a polyphagous herbivore is able to feed on a large variation of host plants due to the flexibility and diversity of genes involved in digestion and detoxification that are deployed in response to particular host plant species.

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Section: Discussionmentioning

confidence: 99%

An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)

2019

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“…Thus pesticides can pose a considerable risk to nontarget organisms when they enter aquatic environments (Ormad et al 2008; Caquet et al 2013; Davis et al 2013). Furthermore, because multiple pesticides are often applied to crops, chemical cocktails are frequently detected in coastal waters, where they may act synergistically or additively, causing a wide range of adverse health effects in exposed aquatic species (Relyea 2009; Magnusson et al 2010; Nunes et al 2018). Pesticides usually persist at relatively low concentrations in coastal waters and generally exceed water quality guidelines only after periods of heavy rainfall (Davis et al 2012; Gallen et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Polyps of the Jellyfish Aurelia aurita Are Unaffected by Chronic Exposure to a Combination of Pesticides

Olguín-Jacobson

Pitt

Carroll

et al. 2020

Enviro Toxic and Chemistry

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Pesticides are a major contaminant in coastal waters and can cause adverse effects in marine invertebrates such as jellyfish. Most studies have investigated short‐term responses of organisms to unrealistically high concentrations of pesticides; however, chronic exposure to persistent low concentrations, which are more likely to occur in the environment, are rarely analyzed. We tested the response of polyps of the moon jellyfish Aurelia aurita to environmental concentrations of the herbicide atrazine and the insecticide chlorpyrifos, individually and in combination, over 9 wk. We hypothesized that exposure to individual pesticides would reduce rates of asexual reproduction and alter polyps' metabolite profiles, and that the results would be more severe when polyps were exposed to the combined pesticides. Polyps survived and reproduced (through budding) in all treatments, and no differences among treatments were observed. Proton nuclear magnetic resonance spectroscopy revealed no difference in profiles of polar metabolites of polyps exposed to the individual or combined pesticides. Our results suggest that A. aurita polyps are unaffected by chronic exposure to atrazine and chlorpyrifos at concentrations recommended as being protective by current Australian water quality guidelines. Environ Toxicol Chem 2020;39:1685–1692. © 2020 SETAC

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“…These include high synteny with the human genome, optical clarity of embryos, highly developed immune system, rapid life cycle, and ease of genetic modification, among others. 64 In Brazil, the zebrafish has been used to characterize the mechanism of action and toxicity of several chemicals, such as metals, [65][66][67] pesticides, [68][69][70] pharmaceutical compounds, [71][72][73] and nanomaterial. [74][75][76] Pharmacology & Toxicology is the second largest area researched in Brazil, and accounts for 16.9% of the articles published up to 2019, contrary to what is observed worldwide, where more developmental biology, cellular biology, molecular biology, biochemistry, and genetics studies have been published.…”

Section: Research Areasmentioning

confidence: 99%

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

et al. 2020

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The zebrafish (Danio rerio) is an emerging model system in several research areas worldwide, especially in the Global South. In this context, the present study revised the historical use and trends of zebrafish as experimental models in Brazil. The data concerning the bibliometric parameters, research areas, geographic distribution, experimental design, zebrafish strain, and reporter lines, as well as recent advances were revised. In addition, the comparative trends of Brazilian and global research were discussed. Revised data showed the rapid growth of Brazilian scientific production using zebrafish as a model, especially in three main research areas (Neuroscience &and Behavior, Pharmacology and Toxicology, and Environment/Ecology). Studies were conducted in 19 Brazilian states (70.37%), confirming the wide geographic distribution and importance of zebrafish research. Results indicated that research related to toxicological approaches are widespread in Global South countries such as Brazil. Studies were performed mainly using in vivo tests (89.58%) with adult fish (59.75%) and embryos (30.67%). Moreover, significant research gaps and recommendations for future research are presented. The present study shows that the zebrafish is a suitable vertebrate model system in the Global South.

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Oxidative effects of the acute exposure to a pesticide mixture of cypermethrin and chlorpyrifos on carp and zebrafish – A comparative study

Cited by 35 publications

References 35 publications

An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)

An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)

Polyps of the Jellyfish Aurelia aurita Are Unaffected by Chronic Exposure to a Combination of Pesticides

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

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