Oxidative effects of inorganic and organic contaminants on haemolymph of mussels

Kaloyianni, Martha; Dailianis, Stefanos; Chrisikopoulou, E.; Zannou, A.; Koutsogiannaki, Sophia; Alamdari, Daryoush Hamidi; Koliakos, George; Dimitriadis, V.K.

doi:10.1016/j.cbpc.2009.01.006

Cited by 49 publications

(39 citation statements)

References 86 publications

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“…According to the results of the present study, oxidative stress induced by Cd at micromolar concentrations, enhances both glutathionylation and protein carbonylation in haemocytes of the mussel M. galloprovincialis. The increased levels of carbonylation in haemocytes of Cd-exposed mussels are in accordance with previously published studies, reporting the carbonylation of proteins in tissues of mussels exposed to heavy metals such as zinc, copper and Cd (Kirchin et al, 1992;Kaloyianni et al, 2009). Actin is considered to be one of the main proteins to be carbonylated in tissues of mussels (McDonagh et al, 2005;McDonagh and Sheehan, 2007).…”

Section: Discussionsupporting

confidence: 91%

“…Heavy metals, such as cadmium (Cd), are considered as potent catalysts in the oxidative deterioration of biological molecules and their toxicity may, at least in part, be caused by the production of reactive oxygen species (ROS) and perturbation of anti-oxidant efficiency (Stacey et al, 1980;Winston, 1991;Stohs and Bagchi, 1995;Pourahmad and O'Brien, 2000;Micic et al, 2001;Dailianis et al, 2005;Kaloyianni et al, 2009). In order to maintain their physiological functioning in the presence of heavy metals toxicity cells have developed antioxidant mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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The role of signalling molecules on actin glutathionylation and protein carbonylation induced by cadmium in haemocytes of musselMytilus galloprovincialis(Lmk)

Dailianis

Patetsini

Kaloyianni

2009

Journal of Experimental Biology

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SUMMARYThis study investigated the role of Na + /H + exchanger (NHE) and signalling molecules, such as cAMP, PKC, PI 3-kinase, and immune defence enzymes, NADPH oxidase and nitric oxide synthase, in the induction of protein glutathionylation and carbonylation in cadmium-treated haemocytes of mussel Mytilus galloprovincialis. Glutathionylation was detected by western blot analysis and showed actin as its main target. A significant increase of both actin glutathionylation and protein carbonylation, were observed in haemocytes exposed to micromolar concentration of cadmium chloride (5moll -1 ). Cadmium seems to cause actin polymerization that may lead to its increased glutathionylation, probably to protect it from cadmium-induced oxidative stress. It is therefore possible that polymerization of actin plays a signalling role in the induction of both glutathionylation and carbonylation processes. NHE seems to play a regulatory role in the induction of oxidative damage and actin glutathionylation, since its inhibition by 2moll -1 cariporide, significantly diminished cadmium effects in each case. Similarly, attenuation of cadmium effects were observed in cells pre-treated with either 11moll -1 GF-109203X, a potent inhibitor of PKC, 50nmoll -1 wortmannin, an inhibitor of PI 3-kinase, 0.01mmoll -1 forskolin, an adenylyl cyclase activator, 10moll -1 DPI, a NADPH oxidase inhibitor, or 10moll -1 L-NAME, a nitric oxide synthase inhibitor, suggesting a possible role of PKC, PI 3-kinase and cAMP, as well as NADPH oxidase and nitric oxide synthase in the enhancement of cadmium effects on both actin glutathionylation and protein carbonylation.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

The role of signalling molecules on actin glutathionylation and protein carbonylation induced by cadmium in haemocytes of musselMytilus galloprovincialis(Lmk)

Dailianis

Patetsini

Kaloyianni

2009

Journal of Experimental Biology

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“…Indeed, evidence shows that various sea shells and fishes indicate perturbed physiology in polluted areas (Zorita et al 2007;Zorita et al 2008). The analysis of toxic compounds such as heavy metals in mussel haemolymph can potentially provide valuable environmental monitoring (Kaloyiannia et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic blood plasma separation in microfluidic channels

Kersaudy-Kerhoas

Dhariwal

Desmulliez

et al. 2009

Microfluid Nanofluid

120

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The separation of red blood cells from plasma flowing in microchannels is possible by biophysical effects such as the Zweifach-Fung bifurcation law. In the present study, daughter channels are placed alongside a main channel such that cells and plasma are collected separately. The device is aimed to be a versatile but yet very simple module producing high-speed and high-efficiency plasma separation. The resulting lab-on-a-chip is manufactured using biocompatible materials. Purity efficiency is measured for mussel and human blood suspensions as different parameters, such as flow rate and geometries of the parent and daughter channels are varied. The issues of blood plasma separation at the microscale are discussed in relation to the different regimes of flow. Results are compared with those obtained by other researchers in the field of micro-separation of blood.

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“…Under normal conditions, the ROS are kept at a low level. However, increases in the cellular ROS level can cause a shift in the balance between oxidation and antioxidation, and induce oxidative damage to membrane lipids, proteins and DNA [37]. Studies have shown that metabolites and reactive intermediates such as diol epoxides, radical cations and redox active o-quinones of PAHs are characterized by high redox potential.…”

Section: Introductionmentioning

confidence: 99%

Effects of pyrene exposure on immune response and oxidative stress in the pearl oyster, Pinctada martensii

Xie

Zhao

Han

et al. 2017

Fish & Shellfish Immunology

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a b s t r a c tPyrene is a polycyclic aromatic hydrocarbon (PAH) commonly observed in aquatic ecosystems, which originates primarily from the incomplete combustion of fossil fuels and the use of petroleum compounds. Pyrene can cause the immune disturbance and oxidative stress, result in immunotoxicity, DNA damage, reduce reproduction significantly, and induce behavioral changes. Marine bivalves are commonly used as bioindicators for marine pollution, and hemolymph is a metabolite transfer medium for PAH pollutant. However, the vital immune indicator responses of pearl oyster Pinctada martensii hemolymph exposed to pyrene is still unclear. Thus, the immunotoxic responses of pyrene on the hemolymph of the Pinctada martensii were investigated in this study. After exposure to pyrene for 7 days, the total number of hemocytes (THC), cell membrane stability (CMS), phagocytic activity (PA) and total glutathione (GSH T ) all decreased significantly. Pyrene also caused a significant increase in lipid peroxi- ) and GSH T (7.2 mg L À1 ), which indicates that THC and LPO were more sensitive. Additionally, a clear dose-effect relationship indicated that pyrene stimulated a marked immune response, as well as oxidative stress in P. martensii, which demonstrates the subtle effects of pyrene exposure on marine invertebrates and the potential associated risk.

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Oxidative effects of inorganic and organic contaminants on haemolymph of mussels

Cited by 49 publications

References 86 publications

The role of signalling molecules on actin glutathionylation and protein carbonylation induced by cadmium in haemocytes of musselMytilus galloprovincialis(Lmk)

The role of signalling molecules on actin glutathionylation and protein carbonylation induced by cadmium in haemocytes of musselMytilus galloprovincialis(Lmk)

Hydrodynamic blood plasma separation in microfluidic channels

Effects of pyrene exposure on immune response and oxidative stress in the pearl oyster, Pinctada martensii

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