Oxidative DNA Damage and Antioxidant Defense after Reperfusion in Acute Myocardial Infarction

Abstract

Although not correlated with other cardiac markers, plasma level of 8-OHdG shows a decrease after reperfusion therapy in patients with MI, and primary percutaneous transluminal coronary angioplasty seems much more effective than thrombolytic therapy for providing a low level of 8-OHdG.

The effects of percutaneous transluminal coronary intervention on biomarkers of oxidative stress in the erythrocytes of elderly male patients

“…The decreased MDA levels indicated the improvement of the pro-and antioxidant balance in the SAP patients, probably due to the reduction in the number of ischemic events. These observations are consistent with the results of other studies [38,44,54,55]. Bashar and Akhtan [38] demonstrated that the regular treatment of AMI patients led to a significant decrease in oxidative stress parameters.…”

“…In the present study, a more advanced state of atherosclerosis was associated with a greater pro-and antioxidant imbalance. Thus, the level of oxidative stress may be used as an independent predictor of cardiovascular events, in agreement with the observations of other authors [15,40,41,[43][44][45][46][47]. Blankenberg et al [43] found an inverse association between erythrocytic GSH-Px and the risk of cardiovascular events.…”

“…After the restoration of blood flow, vascular cells produce more ROS, which results in ROS-generated reperfusion injury [23,36,45]. Ischemia/reperfusion injury is a major clinical problem due to the initiation of myocardial damage [52], which is why oxidative stress during and after PTCA or EC has been intensively investigated in numerous studies [22,23,44,45,53]. In the present study, the parameters of oxidative stress in the blood remained unchanged immediately after the PTCA and EC procedures.…”

“…Kochiadakis et al [54] also observed changes in plasma total peroxides after PTCA with stenting in CAD patients. Similarly, the levels of 8hydroxydeoxyguanosine, which is a marker of oxidative DNA damage, sharply decreased 3 days after PTCA in AMI patients in the Himmetoglu et al study [44]. Moreover, vitamin C infusion 2-6 hours before PTCA resulted in significantly lower serum values of 8-hydroxydeoxyguanosine after the procedure [55].…”

Effects of Oral Glutathione Supplementation on Systemic Oxidative Stress Biomarkers in Human Volunteers

“…However, immunoassays for measurement of 8-OHdG and F2-isoP have been criticized, although results employing this technology being widely published. [26][27][28] Recent publications critiquing ELISA-based 8-OHdG methods suggest that ELISA-based values tended to be higher values than ''gold-standard'' LC/ MS-based measurement. 29 However, several steps were taken in this trial protocol to reduce sources of variability that should have improved the ability to detect a trend for change, if one was present.…”