OXIDATIVE DISSIMILATION OF AMINO ACIDS AND RELATED COMPOUNDS BY
            <i>SHIGELLA FLEXNERI</i>

Erlandson, Arvid L.; Ruhl, Robert F.

doi:10.1128/jb.72.5.708-712.1956

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…The utilization of stereo-isomers of aspartic acid for the growth was investigated on a limited basis, since previous metabolic studies (Erlandson and Ruhl, 1956) had indicated that the D-isomer of aspartic acid inhibited the oxidationi of the L-isomer, the extent of inhibition being dependent on the concentration of the D-isomer present. No such inhibition was found nutritionally.…”

Section: Resultsmentioning

confidence: 99%

“…Since previous evidence (Erlandson and Ruhl, 1956) indicated that aspartic acid, glutamic acid, and asparagine are oxidized at significant rates by nonproliferating cells of Shigella, the question arose as to whether the organism could utilize one of these compounds not only as a nitrogen source, but as a carbon and energy source as well. No growth was obtained with either glutamic acid or asparagine as the carbon, nitrogen and energy source and only sparse growth was obtained using aspartic acid.…”

Section: Resultsmentioning

confidence: 99%

“…However, no inhibition of the natural isomer by the D-isomer was found. It was of interest to note that of all the nitrogen compounds tested, those compounds which supported the growth of the test organism, when singly supplied as the nitrogen source, were qualitatively correlated with those compounds which were actively oxidized and deaminated by nonproliferating cells (Erlandson and Ruhl, 1956). This correlation, however, appeared strictly qualitative and no quantitative correlation was evident.…”

Section: Nutrition Of Shigellamentioning

confidence: 98%

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NUTRITION OF SHIGELLA: GROWTH OF SHIGELLA FLEXNERI IN A SIMPLE CHEMICALLY DEFINED MEDIUM

Erlandson

Mackey

1958

J Bacteriol

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The nutritional requirements of Shigella grown in chemically defined media have been investigated by Koser et at. (1938a, b), Kligler and Grosowitz (1939), and Weil and Black (1944), among others. These investigators have reported the successful cultivation of various strains of Shigella in chemically defined, synthetic media of variable composition. All of these media consisted of inorganic salts, glucose, nicotinic acid, and various combinations of several amino acids. Recent metabolic studies with Shigella flexneri 3 strain 1013, (Erlandson and Ruhl, 1956) have shown that this organism possesses a very active metabolic mechanism for dissimilating amino acids and related nitrogen compounds. Thus, the previously described synthetic media seemed unduly complex for the growth of S. flexneri 3 by reason of the number of amino acids required in their preparation. Consequently, the present study was undertaken to determine whether a single amino acid or other nitrogen compound could be utilized as the nitrogen source for growth and subsequently to develop a simple, chemically defined medium for the cultivation of Shigella. Interest in this laboratory has centered on S. flexneri 3 strain 1013, which was used in previous studies. The nutritional requirements of this culture were studied extensively and the findings applied subsequently to other serotypes and species of Shigella. EXPERIMENTAL METHODS Strain 1013 of Shigella flexneri 3 was employed routinely throughout the experimental study and all data refer to this organism, unless spe-1 The opinions or assertions contained herein are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. A preliminary report of this work appeared previously (Erlandson and Mackey, 1957).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Nutrition Of Shigellamentioning

confidence: 98%

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NUTRITION OF SHIGELLA: GROWTH OF SHIGELLA FLEXNERI IN A SIMPLE CHEMICALLY DEFINED MEDIUM

Erlandson

Mackey

1958

J Bacteriol

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“…Manometric studies. Resting cell suspensions were prepared as previously described (Erlandson and Ruhl, 1956). Approximately 0.4 mg of cellular nitrogen was used per Warburg vessel and all reactions were run aerobically at pH 7.0 and 37 C.…”

Section: Experimental Methodsmentioning

confidence: 99%

GROWTH AND MANOMETRIC STUDIES ON CARBOHYDRATE UTILIZATION BY SHIGELLA FLEXNERI

Erlandson

Mackey

1958

J Bacteriol

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“…It is known that resting cells of Shigellaflexneri 3 are capable of oxidizing glutamate (Erlandson and Ruhl, 1956) but the pathway through which this takes place has not as yet been described. It is the purpose of the present report to show that glutamate is oxidized via the tricarboxylic acid (TCA) cycle by a strain of S. flexneri 3.…”

mentioning

confidence: 99%