Abstract:Biosynthetically produced alkenes are high-value molecules that can serve as ‘drop-in’ replacements for fossil fuels. Alkenes are also heavily used in the polymer, lubricant, and detergent industries. UndB is the only known membrane-bound fatty acid decarboxylase that catalyzes the conversion of fatty acids to terminal alkenes at the highest reported in vivo titers. However, the enzyme remains poorly understood and enigmatic. Here, we demonstrate the first-time purification of UndB and establish that it is an … Show more
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