Oxidative damage on the testes of adult rats by sodium metabisulfite (MBS)

Abstract

The effect of sodium metabisulfite (MBS, Na 2 S 2 O 5 ) on the level of malondialdehyde (MDA), testes weight and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) as well as the level of glutathione (GSH) were investigated in the testes of Wistar albino rats. The study was performed to elucidate the effect of MBS (520 mg/kg/day) ingestion on lipid peroxidation and the antioxidant enzymes activities. The results showed that MDA level as well as the activity of SOD was significantly increased in MBS-treated rats whereas the weight of the testes as well as the level of GSH was not significantly affected. However, there was significant reduction in the activity of CAT in the MBS-treated rats compared to the control. The presented data showed that ingestion of MBS has adverse effect on testes as it induced oxidative damage, reflected by increase lipid peroxidation and impairment in the activities of antioxidant enzymes.

Pathological study on the effect of some food additives in male albino rats

“…The free radicals generated can damage nucleic acids and induce mutation according to Meng, (2003) and Meng and Bai, (2004). These results agreed with Mohamad (2011) and Adebayo, and ADenuga (2012). The brain showed tygrolysis and severe degree of encephalomalacia.…”

Sodium metabisulfite-induced changes on testes, spermatogenesis and epididymal morphometric values in adult rats

“…It is estimated that daily sulfite intake from foods and beverages is 180–200 mg/Kg body weight, which is not in agreement with acceptable daily intake level of 0.7 mg/kg body weight ( 11 ). Several studies have shown that ingestion of SMB at a dose of 520mg/kg/day induced oxidative damage in the rats’ testes, liver, and kidney ( 1 , 12 ). The doses of 10,100, and 260 mg/kg/day sulfite were selected from previous studies ( 6 , 13), designed to represent human exposure to different levels of sulfite, which occurred through the consumption of certain foods and drugs.…”

“…Sulfite salts and sulphur dioxide are added as preservatives to a variety of foods, such as biscuits, chocolate, jam, and in many alcoholic beverages such as beer, wine, etc. and in medications such as parenteral amino acid solutions ( 1 ). Endogenous sulfites are generated in vivo by the catabolism of sulfur-containing amino acids, cysteine and methionine ( 2 ).…”

“…Federation of American Societies for Experimental Biology estimates a "no-observed-adverse effect level" of 30-100 mg sulfur dioxide in humans ( 3 ). Some studies have indicated that sulfites produce toxic effects on the reproductive system, the respiratory system ( 4 ), the nervous system (5) and can also induce allergic reactions ( 1 ). In earlier studies, it has been reported that sodium metabisulfite and sulfur dioxide cause an increase in the lipid peroxidation ( 2 , 6 ).…”

“…Testis, the organ that produces sperm and androgens, is sensitive to a variety of stressors and agents that induce germ cell apoptosis ( 1 ). Understanding these agents and studying the effects of these factors on the male reproductive function is essential in the maintenance of male fertility.…”