Oxidative damage and mitochondrial functionality in hearts from KO UCP3 mice housed at thermoneutrality

Napolitano, Gaetana; Fasciolo, Gianluca; Magnacca, Nunzia; Goglia, Fernando; Lombardi, Assunta; Venditti, Paola

doi:10.1007/s13105-022-00882-9

Cited by 6 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upregulation of UCP3 in skeletal muscle protects against diet‐induced insulin resistance in mice and diabetic patients' UCP3 is reduced by 50% compared with health men [16]. Ablation of UCP3 in mice makes the heart more susceptible to oxidative stress insult by decreasing the oxygen consumption rate and antioxidant enzyme activity and by inducing mitochondrial structural changes thus increases the incidence of myocardial ischemia–reperfusion injury [17,18].…”

mentioning

confidence: 99%

Human mitochondrial uncoupling protein 3 functions as a metabolite transporter

De Leonardis,

Ahmed,

Vozza

et al. 2023

FEBS Letters

View full text Add to dashboard Cite

Since its discovery, a major debate about mitochondrial uncoupling protein 3 (UCP3) has been whether its metabolic actions result primarily from mitochondrial inner membrane proton transport, a process that decreases respiratory efficiency and ATP synthesis. However, UCP3 expression and activity are induced by conditions that would seem at odds with inefficient ‘uncoupled’ respiration, including fasting and exercise. Here, we demonstrate that the bacterially expressed human UCP3, reconstituted into liposomes, catalyses a strict exchange of aspartate, malate, sulphate and phosphate. The R282Q mutation abolishes the transport activity of the protein. Although the substrate specificity and inhibitor sensitivity of UCP3 display similarity with that of its close homolog UCP2, the two proteins significantly differ in their transport mode and kinetic constants.

show abstract

mentioning

confidence: 99%

Human mitochondrial uncoupling protein 3 functions as a metabolite transporter

De Leonardis,

Ahmed,

Vozza

et al. 2023

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Uncoupling proteins (UCPs) are inner membrane carrier proteins that can induce proton leakage and dissipate proton motive force. Several pieces of evidence suggest that mild uncoupling is mediated by uncoupling proteins 2 and 3 (UCP2 and UCP3) that control the production of ROS, decreasing membrane potential [49], therefore reducing oxidative damage [50].…”

Section: Mitochondrial Production Of Ros and Their Role In Agingmentioning

confidence: 99%

Changes in the Mitochondria in the Aging Process—Can α-Tocopherol Affect Them?

Napolitano

Fasciolo

Tomajoli

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Aerobic organisms use molecular oxygen in several reactions, including those in which the oxidation of substrate molecules is coupled to oxygen reduction to produce large amounts of metabolic energy. The utilization of oxygen is associated with the production of ROS, which can damage biological macromolecules but also act as signaling molecules, regulating numerous cellular processes. Mitochondria are the cellular sites where most of the metabolic energy is produced and perform numerous physiological functions by acting as regulatory hubs of cellular metabolism. They retain the remnants of their bacterial ancestors, including an independent genome that encodes part of their protein equipment; they have an accurate quality control system; and control of cellular functions also depends on communication with the nucleus. During aging, mitochondria can undergo dysfunctions, some of which are mediated by ROS. In this review, after a description of how aging affects the mitochondrial quality and quality control system and the involvement of mitochondria in inflammation, we report information on how vitamin E, the main fat-soluble antioxidant, can protect mitochondria from age-related changes. The information in this regard is scarce and limited to some tissues and some aspects of mitochondrial alterations in aging. Improving knowledge of the effects of vitamin E on aging is essential to defining an optimal strategy for healthy aging.

show abstract

“…The content of ROS was measured following the fluorescence of dichlorofluorescein after ROS-induced conversion of 2 ′ ,7 ′ -dichlorodihydrofluorescin diacetate (non-fluorescent compound) in dichlorofluorescein (fluorescent compound) according to Driver et al (2000) and modified by Napolitano et al (2022). The fluorescence was measured using a multi-mode microplate reader (Synergy™ HTX Multi-Mode Microplate Reader, BioTek) with excitation and emission wave at 485 and 530 nm, respectively.…”

Section: Tissue Ros Contentmentioning

confidence: 99%