Oxidative damage and antioxidative indicators in 48 h germinated rice embryos during the vitrification–cryopreservation procedure

Abstract

Cu/Zn SOD and other genes may be critical indicators of a stress response to reactive oxygen species (ROS) accumulation in 48 h germinated rice embryos subjected to vitrification cryopreservation. In the current study, reactive oxygen species (ROS) accumulation was investigated in 48 h germinated rice embryos during the vitrification-cryopreservation process. We found that vitrification-cryopreservation significantly affected ROS levels, especially superoxide anion levels, in 48 h germinated rice embryos. Malonaldehyde content in the apical meristems of germinated embryos was significantly positively correlated with the rate of superoxide anion generation and the highest levels of malonaldehyde content were reached after vitrification treatment. Cell viability in 48 h germinated embryos was significantly negatively correlated with the rate of superoxide anion generation, malonaldehyde content, and electrolyte leakage. Spatial and temporal patterns in ROS accumulation in these embryos existed during the vitrification procedure. Among the vitrification-cryopreservation treatments we assessed, the preculture treatment was found to stimulate superoxide anion generation and to activate the response system in the apical meristems of germinated embryos. Loading treatments motivated the catalase and ascorbate peroxidase activities. During the vitrification-dehydration treatment, oxidative stress reached the highest levels causing an antioxidative response. This response involved antioxidant enzymes promoting detoxification of ROS. Based on a comprehensive correlation analysis involving ROS accumulation, cell viability, the activities of antioxidant enzymes, and gene expression profiles, Cu/Zn SOD, CAT1, APX7, GR2, GR3, MDHAR1, and DHAR1 may be critical indicators of oxidative stress affected by the vitrification-cryopreservation treatments. The investigation of these antioxidative responses in 48 h germinated rice embryos may, therefore, provide useful information with respect to plant vitrification-cryopreservation.

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

“…Membrane lipids are the primary target in oxidative damage [ 54 ], and MDA acting as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa [ 52 , 55 ], Azadirachta indica [ 56 ], Arabidopsis thaliana [ 8 ], Agapanthus praecox [ 10 ], Hancornia speciose [ 57 ], and Passiflora suberosa [ 53 ]. With the addition of NRSP in the cryoprotectant, the MDA content decreased and the SOD activity increased leading to more intact membrane and better quality of boar sperm [ 21 ].…”

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

“…Effective antioxidant response during dehydration step improved EC survival after cryopreservation ROS-induced oxidative stress is a major reason of low survival in samples after cryopreservation [40][41][42][43][44][45][46][47][48]. In many species cryopreservation, H 2 O 2 is the major component of ROS leading to oxidative stress [8,45].…”

“…Membrane lipids are the primary target in oxidative damage [49], and MDA acting as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa [47,50], Azadirachta indica [51], Arabidopsis thaliana [8] and Agapanthus praecox [44], Hancornia speciose [52], Passi ora suberosa [48].…”

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

“…Effective antioxidant response during the dehydration step improved EC survival after cryopreservation ROS-induced oxidative stress is a major reason of low survival in samples after cryopreservation [46][47][48][49][50][51][52][53][54]. In many species cryopreservation, H 2 O 2 is the major component of ROS leading to oxidative stress [8,51].…”

“…Membrane lipids are the primary target in oxidative damage [55], and MDA acting as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa [53,56], Azadirachtaindica [57], Arabidopsisthaliana [8], Agapanthus praecox [50], Hancornia speciose [58], and Passi ora suberosa [54]. With the addition of NRSP in the cryoprotectant, the MDA content decreased and the SOD activity increased leading to more intact membrane and better quality of boar sperm [21].…”