Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide

Abstract

Rapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colored product absorbed at maximum 434 nm. Regression analysis of linearity range was found (0.6-2.8) µg.ml-1. The optimum conditions that affect the oxidation were studied. The developed method was found to be precise with mean value of relative standard deviation (1.153- 1.303) and accurate with relative error (-0.5940-1.7821) .The calculated molar absorptivity and sandal sensitivity values of (29825 L.mol-1.cm-1), 0.0099 µg.cm-2 respectively. The limit of detection and limit of quantitation were of 0.04696, 0.156548 µg.ml-1 respectively .The suggested method showed good recovery with a mean value of 100.77% for analysis of dosage forms.

A New Green Approach of CFIA Technique for Direct Assay with a High Throughput of Sulfamethoxazole Drugs Using Condensation Reaction with NQS Agent

“…Several methods have been described for SMZ drug estimation like flow injection analysis 4 . selective electroed 5 , spectrophotometric method [6][7][8] , HPLC [9][10][11] , and fluorometry 13 in this manuscript, an accurate, sensitive, and simple of semiautomated flow injection analysis CFIA/MZ technique [14][15][16][17][18] were used to examine the quantitate…”

Indirect spectrophotometric determination of Mebendazole using n-bromosuccinimide as an oxidant and tartarazine dye as analytical reagent

“…64, No. 9 (2021) bromoaniline in the presence of N-bromosuccinimide [15]. MBZ in Vermox Plus tablets was determined by high performance thin layer chromatography (HPTLC) and RP-HPLC using isocratic separation [16].…”