Oxidative changes in the blood and serum albumin differentiate rats with monoarthritis and polyarthritis

Abstract

Adjuvant arthritis in rats, as rheumatoid arthritis in humans, may be of greater or lesser severity, namely polyarthritis and monoarthritis, respectively. The present study was planned to evaluate the oxidative changes in the blood and specifically in the serum albumin of rats with adjuvant-induced mono- and poly-arthritis. Total antioxidant capacity, thiols, carbonyl groups, albumin, uric acid and ascorbic acid were measured in the total serum. The specific oxidative status of albumin was also measured after separation by affinity chromatography. All serum oxidative parameters were close to normal in monoarthritic rats with the exception of the ascorbic acid concentration, which was 23 % lower, and albumin carbonyl groups, which were 64 % higher. Many modifications were found in polyarthritic rats, specially the ascorbic acid concentration (35 % lower) and albumin carbonyl groups (102 % higher). The results revealed that the levels of ascorbic acid in the serum and carbonyl groups in the albumin molecule can be regarded as indicators of the severity of arthritis since they were modified by both monoarthritis and polyarthritis, but to different degrees.

β‐Caryophyllene, the major constituent of copaiba oil, reduces systemic inflammation and oxidative stress in arthritic rats

“…Similarly, a smaller ROS production after β-caryophyllene treatment would be expected. 16 In the current study, the plasmatic levels of albumin and thiols were reduced in the same proportion by arthritis and the treatment with β-caryophyllene did not increase them. 42 The same can be said about the respiration of isolated mitochondria, which is modified by changes in the redox state.…”

“…12 Metabolic modifications are equally significant in the liver of rats with adjuvant-induced arthritis, such as increased glycolysis and reduced gluconeogenesis. [14][15][16] Particularly in the liver, where inflammation and metabolic alterations are prominent, oxidative stress is quite pronounced when compared with other organs. [14][15][16] Particularly in the liver, where inflammation and metabolic alterations are prominent, oxidative stress is quite pronounced when compared with other organs.…”

Anti‐Inflammatory and Antioxidant Actions of Copaiba Oil Are Related to Liver Cell Modifications in Arthritic Rats

“…The copaiba oil, however, was not able to decrease the oxidative stress in the plasma of arthritic rats, where antioxidant enzymes and glutathione contribute poorly and the antioxidant activity depends mainly on thiol groups of albumin [Bracht et al, ]. To albumin alone 70% of the antioxidant capacity of the plasma has been attributed and this protein contributes with 80% to the plasmatic thiol groups [Bracht et al, ]. In the present study, the levels of albumin, thiols, and the total antioxidant capacity in the plasma were both decreased in the same proportion by arthritis, a finding that corroborates previous observations [Bracht et al, ].…”

“…Rheumatoid arthritis is a systemic disease and in addition to the joints other organs are affected, such as brain, heart, lungs, and vascular tissue [McInnes and Schett, 2011]. The oxidative status is likewise changed in the serum blood of patients with rheumatoid arthritis and also in the liver, brain, and heart of rats with adjuvant arthritis [Lemarechal et al, 2006;Comar et al, 2013;Wendt et al, 2015;Bracht et al, 2016;Schubert et al, 2016].…”

Hesperidin and Experimentally-Induced Arthritis in Male Rats

“…Different inflammatory cell types as macrophages and neutrophils promote the formation of reactive oxygen species (ROS) which are involved in the progression of RA. There are higher levels of oxidative markers both in serum of RA patients (37) and also in liver of rats with experimental arthritis (38) . Lipid peroxidation, protein oxidation, DNA damage and failure of antioxidant defense system lead to tissue damage and chronicity of the disease (39) .…”