Oxidative burst assessment and neutrophil–platelet complexes in unlysed whole blood

Avendaño, Ariadna; Sales-Pardo, Irene; Marin, Lorena; Marı́n, Pedro; Pétriz, Jordi

doi:10.1016/j.jim.2008.09.003

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…To facilitate comparison with our studies in mice (18), we assessed neutrophil function using PMA as the stimulus for the oxidative burst. Although this is not a physiological stimulus, the advantages of this method are: i) that it produces a strong oxidative burst (55-56) which is clearly distinguished from any low-level activation caused by malaria infection per se , ii) it is not dependent on phagocytosis (which might also be impaired by malaria) (55-56), and iii) variations in the magnitude of the PMA-induced oxidative burst are directly related to the ability of humans to survive infections (57). …”

Section: Discussionmentioning

confidence: 99%

Prolonged Neutrophil Dysfunction after Plasmodium falciparum Malaria Is Related to Hemolysis and Heme Oxygenase-1 Induction

Cunnington

Njie

Correa

et al. 2012

The Journal of Immunology

109

124

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It is not known why people are more susceptible to bacterial infections such as non-Typhoid Salmonella (NTS) during and after a malaria infection but, in mice, malarial hemolysis impairs resistance to NTS by impairing the neutrophil oxidative burst. This acquired neutrophil dysfunction is a consequence of induction of the cytoprotective, heme degrading enzyme heme oxygenase-1 (HO-1) in neutrophil progenitors in bone marrow. In this study, we assessed whether neutrophil dysfunction occurs in humans with malaria and how this relates to hemolysis. We evaluated neutrophil function in 58 Gambian children with Plasmodium falciparum malaria (55 (95%) with uncomplicated disease), and examined associations with erythrocyte count, haptoglobin, hemopexin, plasma heme, expression of receptors for heme uptake, and HO-1 induction. Malaria caused the appearance of a dominant population of neutrophils with reduced oxidative burst activity, which gradually normalized over 8 weeks of follow-up. The degree of neutrophil impairment correlated significantly with markers of hemolysis and HO-1 induction. HO-1 expression was increased in blood during acute malaria, but at a cellular level HO-1 expression was modulated by changes in surface expression of the haptoglobin receptor (CD163). These findings demonstrate that neutrophil dysfunction occurs in P. falciparum malaria and support the relevance of the mechanistic studies in mice. Furthermore, they suggest the presence of a regulatory pathway to limit HO-1 induction by hemolysis in the context of infection, and indicate new targets for therapeutic intervention to abrogate the susceptibility to bacterial infection in the context of hemolysis in humans.

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Section: Discussionmentioning

confidence: 99%

Prolonged Neutrophil Dysfunction after Plasmodium falciparum Malaria Is Related to Hemolysis and Heme Oxygenase-1 Induction

Cunnington

Njie

Correa

et al. 2012

The Journal of Immunology

109

124

View full text Add to dashboard Cite

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“…Among the reactive oxygen intermediates H 2 O 2 is the most stable product [31] and thus a reliable component to quantify in measurements of respiratory burst activity. DHR has been used in assays in a range of species, like man [32,33], dog [34], cattle [35], mouse [36] and also in mussel [37]. In fish, DHR has been used for several species, like trout [38], cod [39], goldfish [40,41], sea bream [30], flounder [42] and ayu [29].…”

Section: Introductionmentioning

confidence: 99%

Flow cytometry assays of respiratory burst in Atlantic salmon (Salmo salar L.) and in Atlantic cod (Gadus morhua L.) leucocytes

Kalgraff

Wergeland

Pettersen

2011

Fish & Shellfish Immunology

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“…Repeated control groups were avoided, and a randomised block plot could be applied during statistical assessment. Although a variety of drugs have been developed to minimise the post-operative inflammatory response, only a few of them have been tested under CPB circumstances (20,21). The sham CPB model is the model usually used in such pre-clinical testing (9)(10)(11).…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Model for Studying Neutrophil Activation During Cardiopulmonary Bypass by Using a Polymerase Chain Reaction Thermocycler

Tang

Zhao²,

Gu³

et al. 2010

Altern Lab Anim

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The accurate temperature control of a polymerase chain reaction (PCR) thermocycler was exploited in developing an in vitro model to study neutrophil activation during cardiopulmonary bypass. Neutrophils from 12 volunteers underwent temperature changes in a PCR thermocycler (37°C for 30 minutes, 28°C for 60 minutes, and then 37°C for 90 minutes). Different co-incubates were applied to neutrophils, as follows: Group A: phosphate-buffered saline solution; Group B: platelet activating factor (PAF); Group C: platelet-depleted plasma; Group D: platelet-depleted plasma + PAF; and Group E: platelet-rich plasma. Membrane-bound elastase (MBE) activity was measured every 30 minutes throughout the experiment. MBE acticity decreased significantly after hypothermia, compared with the baseline level (p < 0.001), and it resumed an increase after re-warming. Among all co-incubates, platelet-rich plasma was the most potent pro-inflammatory stimulus to neutrophils. A linear correlation was found between MBE and platelet count in platelet-rich plasma (p = 0.004). A novel in vitro model involving a PCR thermocycler has been proved to be reliable in the study of neutrophil activation during cardiopulmonary bypass. The model could possibly be used as an alternative to animals in the development of new drugs to combat neutrophil damage to tissues and organs during cardiopulmonary bypass in cardiac surgery.

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Oxidative burst assessment and neutrophil–platelet complexes in unlysed whole blood

Cited by 13 publications

References 33 publications

Prolonged Neutrophil Dysfunction after Plasmodium falciparum Malaria Is Related to Hemolysis and Heme Oxygenase-1 Induction

Prolonged Neutrophil Dysfunction after Plasmodium falciparum Malaria Is Related to Hemolysis and Heme Oxygenase-1 Induction

Flow cytometry assays of respiratory burst in Atlantic salmon (Salmo salar L.) and in Atlantic cod (Gadus morhua L.) leucocytes

An In Vitro Model for Studying Neutrophil Activation During Cardiopulmonary Bypass by Using a Polymerase Chain Reaction Thermocycler

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