Oxidative Burst and Hypoosmotic Stress in Tobacco Cell Suspensions

Cazalé, Anne‐Claire; Rouet-Mayer, Marie-Aude; Barbier‐Brygoo, Hélène; Mathieu, Yves; Laurière, Christiane

doi:10.1104/pp.116.2.659

Cited by 119 publications

(114 citation statements)

References 59 publications

(24 reference statements)

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…DPI has been reported to inhibit the oxidative burst in plants (Levine et al, 1994;Cazalé et al, 1998;Papadakis and Roubelakis-Angelakis, 1999). We find that 5 m DPI effectively inhibited the deposition of GvP1 in response to Pmg, and partial inhibition was observed with 1 m. This inhibitory effect appears to be due to the supression of H 2 O 2 formation because DPI had no effect on GvP1 deposition in response to the addition of exogenous H 2 O 2 .…”

Section: Discussionmentioning

confidence: 57%

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

et al. 2001

View full text Add to dashboard Cite

Elicitation or peroxide stimulation of grape (Vitis vinifera L. cv Touriga) vine callus cultures results in the rapid and selective in situ insolubilization of an abundant and ionically bound cell wall protein-denominated GvP1. Surface-enhanced laser desorption/ionization/time of flight-mass spectrometry analysis, the amino acid composition, and the N-terminal sequence of purified GvP1 identified it as an 89.9-kD extensin. Analysis of cell walls following the in situ insolubilization of GvP1 indicates large and specific increases in the major amino acids of GvP1 as compared with the amino acids present in salt-eluted cell walls. We calculate that following deposition, covalently bound GvP1 contributes up to 4% to 5% of the cell wall dry weight. The deposition of GvP1 in situ requires peroxide and endogenous peroxidase activity. Isoelectric focusing of saline eluates of callus revealed only a few basic peroxidases that were all isolated or purified to electrophoretic homogeneity. In vitro and in situ assays of extensin cross-linking activity using GvP1 and peroxidases showed that a 40-kD peroxidase cross-linked GvP1 within minutes, whereas other grapevine peroxidases had no significant activity with GvP1. Internal peptide sequences indicated this extensin peroxidase (EP) is a member of the class III peroxidases. We conclude that we have identified and purified an EP from grapevine callus that is responsible for the catalysis of GvP1 deposition in situ during elicitation. Our results suggest that GvP1 and this EP play an important combined role in grapevine cell wall defense.

show abstract

Section: Discussionmentioning

confidence: 57%

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Because of their ability to regenerate cell walls and their homogenous nature, protoplasts have emerged as a versatile investigative tool for understanding the physiological and genetic aspects of plants in response to experimental treatments and cell wall perturbations (Burgess 1983;Cazale et al 1998;Manfield et al 2004;Pojnar and Cocking 1967;Shea et al 1989;Skopelitis et al 2006;Wang et al 2007;Bart et al 2010). Cell wall regeneration around protoplasts varies in different species ranging from as soon as 10 min following transfer of cells to regeneration medium to *5 days (Amstel and Kengen 1996; Burgess and Fleming 1974;Kwon et al 2005;Yang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

et al. 2011

View full text Add to dashboard Cite

TitleTranscriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice. Abstract Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cells over time. In total, 928 genes exhibited significant up-regulation during cell wall removal, whereas, 79 genes were up-regulated during cell wall regeneration. Both gene sets are enriched for kinases, transcription factors and genes predicted to be involved in cell wall-related functions. Integration of the gene expression datasets with a catalog of known and/or predicted biochemical pathways from rice, revealed metabolic and hormonal pathways involved in cell wall degradation and regeneration. Rice lines carrying Tos17 mutations in genes up-regulated during cell wall removal exhibit dwarf phenotypes. Many of the genes up-regulated during cell wall development are also up-regulated in response to infection and environmental perturbations indicating a coordinated response to diverse types of stress.

show abstract

“…Despite the large electrochemical gradient driving anions outside the cell, anions are accumulated in plant cells in resting conditions. However, signals such as elicitors of plant defense responses, hormones, or hypo-osmotic stress that depolarize the membrane induce anion effluxes that can be accounted for by the activation of anion channels (11)(12)(13)(14). The tight control of anion channel activation by intra-and extracellular factors is thus an important step in signal transduction pathways.…”

mentioning

confidence: 99%

Nucleotides Provide a Voltage-sensitive Gate for the Rapid Anion Channel of Arabidopsis Hypocotyl Cells

Colcombet¹,

Thomine²,

Guern

et al. 2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

The rapid anion channel of Arabidopsis hypocotyl cells is highly voltage-dependent. At hyperpolarized potentials, the channel is closed, and membrane depolarization is required for channel activation. We have previously shown that channel gating is regulated by intracellular nucleotides. In the present study, we further analyze the channel gating, and we propose a mechanism to explain its regulation by voltage. In the absence of intracellular nucleotides, closure at hyperpolarized voltages is abolished. Structure-function studies of adenyl nucleotides show that the apparent gating charge of the current increases with the negative charge carried by nucleotides. We propose that the fast anion channel is gated by the voltage-dependent entry of free nucleotides into the pore, leading to a voltage-dependent block at hyperpolarized potentials. In agreement with this mechanism in which intracellular nucleotides need to be recruited to the channel pore, kinetic analyses of whole-cell and single-channel currents show that the rate of closure is faster when intracellular nucleotide concentration is increased, whereas the rate of channel activation is unchanged. Furthermore, decreasing the concentration of extracellular chloride enhances the intracellular nucleotide block. This result supports the hypothesis of a mechanism in which blocking nucleotides and permeant anions interact within the channel pore.The molecular mechanisms of voltage gating are well understood for some channels. Those mechanisms can vary, but they always involve the movement of charged gating particles through the transmembrane electric field. These gating charges can be intrinsic to the channel protein, as described in potassium, sodium, and calcium channels (1). For instance, in the shaker-like potassium channels described in animal and plant membranes, mutations of basic amino acids of the S4 transmembrane domain drastically change the voltage regulation of the channel, indicating that these amino acids contribute to the gating charge (2). Alternately, voltage-dependent closure can be achieved by voltage-dependent block of the channel pore by a soluble charged molecule. This is the case for NMDA ionotropic receptors (3, 4) and inward rectifying potassium channels (5), which are gated by soluble cations. Except for the channels belonging to the shaker family, the gating mechanisms of plant ion channels remain poorly understood.Plasma membrane anion channels have been identified in a number of different plant tissues (6). Except in guard cells, for which a combination of electrophysiology, pharmacology, and genetic approaches has provided strong evidence that the slowtype anion channel is involved in stomatal closure (7-10), physiological functions of plant anion channels remain to be elucidated. Despite the large electrochemical gradient driving anions outside the cell, anions are accumulated in plant cells in resting conditions. However, signals such as elicitors of plant defense responses, hormones, or hypo-osmotic stress that depolarize the memb...

show abstract

Oxidative Burst and Hypoosmotic Stress in Tobacco Cell Suspensions

Cited by 119 publications

References 59 publications

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

Nucleotides Provide a Voltage-sensitive Gate for the Rapid Anion Channel of Arabidopsis Hypocotyl Cells

Contact Info

Product

Resources

About