Oxidative and other posttranslational modifications in extracellular vesicle biology

Szabó-Taylor, Katalin; Ryan, Brent J.; Osteikoetxea, Xabier; Szabó, Tamás; Sódar, Barbara; Holub, Marianna Csilla; Németh, Andrea H.; Pálóczi, Krisztina; Pállinger, Éva; Winyard, Paul G.; Buzás, Edit I.

doi:10.1016/j.semcdb.2015.02.012

Cited by 43 publications

(36 citation statements)

References 98 publications

(134 reference statements)

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“…Oxidized γ-synuclein is released into exosomes and incorporated in neighbouring cells, amplifying the toxic proteinaggregation cascade that finally causes a degenerative synucleinopathy [118]. Besides, other oxidized proteins appear in EVs as described in other studies (reviewed in [107]) ( Table 2 and Fig. 1).…”

Section: Other Ptms In Evsmentioning

confidence: 70%

“…Nevertheless, ubiquitin and UBLs are not the only PTMs detected in EVs [73,107]. For example, many phosphorylated proteins appear in exosomes, including the calcium-dependent phospholipid-binding protein Annexin A2, which is enriched in cholesterol-rich lipid raft micro-domains of exosome membranes [101,108,109].…”

Section: Other Ptms In Evsmentioning

confidence: 99%

“…1). Moreover, several studies have revealed the presence of glycosylated proteins in EVs (reviewed in [73,107]). Notably, EVs contain mainly mannose or sialic acid enriched-glycans and N-linked glycoproteins.…”

Section: Other Ptms In Evsmentioning

confidence: 99%

“…The function of PTMs such as oxidation, phosphorylation, glycosylation and citrullination in the incorporation of proteins into EVs, has been recently reviewed in [107]. Briefly, these PTMs can regulate EVs release and uptake, acting as a disposal mechanism for cellular harmful components or as a repair mechanism in physio-pathological conditions [107].…”

Section: The Role Of Ubl and Other Ptms In The Sorting Of Proteins Tomentioning

confidence: 99%

“…Briefly, these PTMs can regulate EVs release and uptake, acting as a disposal mechanism for cellular harmful components or as a repair mechanism in physio-pathological conditions [107]. Similar to ubiquitin, the UBL SUMO also plays an important role in protein sorting to EVs.…”

Section: The Role Of Ubl and Other Ptms In The Sorting Of Proteins Tomentioning

confidence: 99%

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Post-translational add-ons mark the path in exosomal protein sorting

Moreno-Gonzalo

Fernández-Delgado

Sánchez‐Madrid

2017

Cell. Mol. Life Sci.

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Section: Other Ptms In Evsmentioning

confidence: 70%

Section: Other Ptms In Evsmentioning

confidence: 99%

Section: Other Ptms In Evsmentioning

confidence: 99%

Section: The Role Of Ubl and Other Ptms In The Sorting Of Proteins Tomentioning

confidence: 99%

Section: The Role Of Ubl and Other Ptms In The Sorting Of Proteins Tomentioning

confidence: 99%

See 3 more Smart Citations

Post-translational add-ons mark the path in exosomal protein sorting

Moreno-Gonzalo

Fernández-Delgado

Sánchez‐Madrid

2017

Cell. Mol. Life Sci.

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Extracellular vesicles in toxicological studies: key roles in communication between environmental stress and adverse outcomes

Qin

Huang

2020

J of Applied Toxicology

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External stressors, especially environmental toxicants can disturb biological homeostasis and thus lead to adverse health effects. However, there is limited understanding of how cells directly exposed to stressors transmit the signals to cells indirectly in contact with stressors. Extracellular vesicles (EVs) are receiving increasing attention as signal transductors between various types of cells in organisms. Cargo in EVs, including RNAs, proteins, lipids, and other signal molecules can be transferred between cells and become critical determining factors of intercellular communication. EVs can be a powerful mediator of environmental stimuli. It has been shown that external stressors reshape the secretion of EVs, modify the composition of EVs, and thus influence the mediating function of EVs. These abnormal EVs can lead to dysfunction of recipient cells, and even the pathogenesis of diseases. In this review, we first summarized current knowledge about the responses of EVs to external stimuli, including chemicals and chemical mixtures. Then we explained how these altered EVs regulate signal pathways in recipient cells, thus mediating physio‐pathological responses in detail. The most up‐to‐date evidence from molecular, cellular, animal and human levels was synthesized to systematically address the mediating roles of EVs. EVs can be regarded as a bridge to link external stressors and internal response. Further toxicological and molecular epidemiological studies are expected to provide further insight into the roles of EVs in toxicology. The gaps in the engulfment of toxicants into EVs are listed as the priority to be solved in future studies.

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Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

et al. 2019

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Issues associated with upscaling exosome production for therapeutic use may be overcome through utilizing artificial exosomes. Cell‐derived mimetic nanovesicles (M‐NVs) are a potentially promising alternative to exosomes for clinical applicability, demonstrating higher yield without incumbent production and isolation issues. Although several studies have shown that M‐NVs have similar morphology, size and therapeutic potential compared to exosomes, comprehensive characterization and to what extent M‐NVs components mimic exosomes remain elusive. M‐NVs were generated through the extrusion of cells and proteomic profiling demonstrated an enrichment of proteins associated with membrane and cytosolic components. The proteomic data herein reveal a subset of proteins that are highly abundant in M‐NVs in comparison to exosomes. M‐NVs contain proteins that largely represent the parental cell proteome, whereas the profile of exosomal proteins highlight their endosomally derived origin. This advantage of M‐NVs alleviates the necessity of endosomal sorting of endogenous therapeutic proteins or RNA into exosomes. This study also highlights differences in protein post‐translational modifications among M‐NVs, as distinct from exosomes. Overall this study provides key insights into defining the proteome composition of M‐NVs as a distinct from exosomes, and the potential advantage of M‐NVs as an alternative nanocarrier when spontaneous endosomal sorting of therapeutics are limited.

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Oxidative and other posttranslational modifications in extracellular vesicle biology

Cited by 43 publications

References 98 publications

Post-translational add-ons mark the path in exosomal protein sorting

Post-translational add-ons mark the path in exosomal protein sorting

Extracellular vesicles in toxicological studies: key roles in communication between environmental stress and adverse outcomes

Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes

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