Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens

JingTing, Shu; Qin, Xiao; Shan, Yanju; Zhang, Ming; Tu, Yan; Ji, Gaige; Sheng, Zhongwei; Zou, Jianmin

doi:10.1371/journal.pone.0183118

Cited by 15 publications

(10 citation statements)

References 47 publications

(63 reference statements)

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“…Interestingly, our data showed up-regulation of several genes, such as MYBPC1 and SMPX [127,128], that are more closely associated with slow-twitch oxidative muscle rather than fast-twitch glycolytic muscle. For example, LMOD2 has been alternatively called cardiac leiomodin and its levels in cardiac muscle are directly linked to the length of actin-containing thin filaments due to competition for binding with tropomodulin-1 [129].…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Lake

Papah

Abasht

2019

Genes

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Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from 101 birds at 14 days of age. Birds were subsequently raised to 7 weeks of age to allow sample selection based on the wooden breast phenotype at market age. RNA-sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p-value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

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Section: Discussionmentioning

confidence: 99%

Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Lake

Papah

Abasht

2019

Genes

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“…The genes related to muscle growth and fat deposition are PPP3CA, DPYD, and FGGY. Some literature reports that calcineurin (PPP3CA) can promote the dephosphorylation of the nuclear factor of activated T cells (NFAT), allowing it to smoothly enter the nucleus from the cytoplasm, and the target gene of NFAT can participate in skeletal muscle differentiation and Hypertrophic Gene Program (Figure 6(c)) [67, 68]. The DPYD gene is involved in β -alanine metabolism and participated in the modification of lysine, and lysine plays an important role in lipid peroxidation and muscle and muscle bond development (Figure 6(d)).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Effect of Long Intergenic Noncoding RNAs on Pig Muscle Growth and Fat Deposition

Chen

Cheng

Shi

et al. 2019

BioMed Research International

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Muscle growth and fat deposition are the two important biological processes in the development of pigs which are closely related to the pig production performance. Long intergenic noncoding RNAs (lincRNAs), with lack of coding potential and the length of at least 200nt, have been extensively studied to play important roles in many biological processes. However, the importance and molecular regulation mechanism of lincRNAs in the process of muscle growth and fat deposition in pigs are still to be further studied comprehensively. In our study, we used the data, including liver, abdominal fat, and longissimus dorsi muscle of 240 days’ age of two F2 full-sib female individuals from the white Duroc and Erhualian crossbreed, to identify 581 putative lincRNAs associated with pig muscle growth and fat deposition. The 581 putative lincRNAs shared many common features with other mammalian lincRNAs, such as fewer exons, lower expression levels, and shorter transcript lengths. Cross-tissue comparisons showed that many transcripts were tissue-specific and were involved in the important biological processes in their corresponding tissues. Gene ontology and pathway analysis revealed that many potential target genes (PTGs) of putative lincRNAs were involved in pig muscle growth and fat deposition-related processes, including muscle cell proliferation, lipid metabolism, and fatty acid degradation. In Quantitative Trait Locus (QTLs) analysis, some PTGs were screened from putative lincRNAs, MRPL12 is associated with muscle growth, GCGR and SLC25A10 were associated with fat deposition, and PPP3CA, DPYD, and FGGY were related not only to muscle growth but also to fat deposition. Therefore, it implied that these lincRNAs might participate in the biological processes related to muscle growth or fat deposition through homeostatic regulation of PTGs, but the detailed molecular regulatory mechanisms still needed to be further explored. This study lays the molecular foundation for the in-depth study of the role of lincRNAs in the pig muscle growth and fat deposition and further provides the new molecular markers for understanding the complex biological mechanisms of pig muscle growth and fat deposition.

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“…Previous studies showed that PRKAG3 was also related to meat quality [ 46 , 47 ]. Shu et al [ 48 ] found that PRKAG3 expression levels were significantly higher in glycolytic skeletal muscle than in oxidative skeletal muscle by transcriptome sequencing, but a higher content of oxidative (red) fibers in muscles can result in higher meat quality [ 49 ]. This suggested that high expression of the PRKAG3 gene may decrease meat quality.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

Dai

Chen

et al. 2018

PLoS ONE

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Chicken is widely favored by consumers because of some unique features. The leg muscles occupy an important position in the market. However, the specific mechanism for regulating muscle growth speed is not clear. In this experiment, we used Jinghai yellow chickens with different body weights at 300 days as research subjects. The chickens were divided into fast- and slow-growing groups, and we collected leg muscles after slaughtering for use in RNA-seq. After comparing the two groups, 87 differentially expressed genes (DEGs) were identified (fold change ≥ 2 and FDR < 0.05). The fast-growing group had 42 up-regulated genes and 45 down-regulated genes among these DEGs compared to the slow-growing group. Six items were significantly enriched in the biological process: embryo development ending in birth or egg hatching, chordate embryonic development, embryonic skeletal system development, and embryo development as well as responses to ketones and the sulfur compound biosynthetic process. Two significantly enriched pathways were found in the KEGG pathway analysis (P-value < 0.05): the insulin signaling pathway and the adipocytokine signaling pathway. This study provides a theoretical basis for the molecular mechanism of chicken growth and for improving the production of Jinghai yellow chicken.

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Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens

Cited by 15 publications

References 47 publications

Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Transcriptome Analysis Reveals the Effect of Long Intergenic Noncoding RNAs on Pig Muscle Growth and Fat Deposition

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

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