Oxidative and genotoxic effects of 900MHz electromagnetic fields in the earthworm Eisenia fetida

Tkalec, Mirta; Štambuk, Anamaria; Šrut, Maja; Malarić, Krešimir; Klobučar, Göran

doi:10.1016/j.ecoenv.2012.12.005

Cited by 39 publications

(15 citation statements)

References 57 publications

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“…Selected in vivo models have been recently used to suit specific scientific questions. For example, Eisenia Fetida (Oligochaeta, heartworm) was selected for standardized assays to evaluate ecological risk in terrestrial ecosystems [52]. This model has been validated for toxicological endpoints of genotoxicity such as DNA damage, anti-oxidative enzyme activities, as well as lipid peroxidation.…”

Section: The Effect Of Rf Exposure In Vivo: New Insights On Biosystemmentioning

confidence: 99%

Searching for the Perfect Wave: The Effect of Radiofrequency Electromagnetic Fields on Cells

Gherardini

Ciuti

Tognarelli

et al. 2014

IJMS

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There is a growing concern in the population about the effects that environmental exposure to any source of “uncontrolled” radiation may have on public health. Anxiety arises from the controversial knowledge about the effect of electromagnetic field (EMF) exposure to cells and organisms but most of all concerning the possible causal relation to human diseases. Here we reviewed those in vitro and in vivo and epidemiological works that gave a new insight about the effect of radio frequency (RF) exposure, relating to intracellular molecular pathways that lead to biological and functional outcomes. It appears that a thorough application of standardized protocols is the key to reliable data acquisition and interpretation that could contribute a clearer picture for scientists and lay public. Moreover, specific tuning of experimental and clinical RF exposure might lead to beneficial health effects.

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Section: The Effect Of Rf Exposure In Vivo: New Insights On Biosystemmentioning

confidence: 99%

Searching for the Perfect Wave: The Effect of Radiofrequency Electromagnetic Fields on Cells

Gherardini

Ciuti

Tognarelli

et al. 2014

IJMS

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“…1/ In E. fetida not only did EMF trigger DNA strand breaks, as previously reported (7), but it also caused DNA modifications such as single base pairs substitutions, deletions, and insertions resulting in the modification of the hybridisation sites of RAPD probes.…”

Section: Discussionmentioning

confidence: 70%

“…In a previous study on E. fetida, it was observed that EMF triggered DNA strand breaks in coelomocytes, as evidenced using the comet assay, as well as the onset of oxidative stress as exemplified by protein carbonylation, lipid peroxidation, increased catalase activity, and increased glutathione reductase activity (7).…”

mentioning

confidence: 91%

Electromagnetic fields at a mobile phone frequency (900 MHz) trigger the onset of general stress response along with DNA modifications in Eisenia fetida earthworms

Bourdineaud

Šrut

Štambuk

et al. 2017

Archives of Industrial Hygiene and Toxicology

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Eisenia fetida earthworms were exposed to electromagnetic field (EMF) at a mobile phone frequency (900 MHz) and at field levels ranging from 10 to 120 V m-1 for a period of two hours (corresponding to specific absorption rates ranging from 0.13 to 9.33 mW kg-1). Potential effects of longer exposure (four hours), field modulation, and a recovery period of 24 h after two hours of exposure were addressed at the field level of 23 V m-1. All exposure treatments induced significant DNA modifications as assessed by a quantitative random amplified polymorphic DNA-PCR. Even after 24 h of recovery following a two hour-exposure, the number of probe hybridisation sites displayed a significant two-fold decrease as compared to untreated control earthworms, implying a loss of hybridisation sites and a persistent genotoxic effect of EMF. Expression of genes involved in the response to general stress (HSP70 encoding the 70 kDa heat shock protein, and MEKK1 involved in signal transduction), oxidative stress (CAT, encoding catalase), and chemical and immune defence (LYS, encoding lysenin, and MYD, encoding a myeloid differentiation factor) were up-regulated after exposure to 10 and modulated 23 V m-1 field levels. Western blots showing an increased quantity of HSP70 and MTCO1 proteins confirmed this stress response. HSP70 and LYS genes were up-regulated after 24 h of recovery following a two hour-exposure, meaning that the effect of EMF exposure lasted for hours.

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“…Organisms possess antioxidant defense systems against oxidative damage caused by ROS (Meral et al 2007, Tkalec et al 2013. Antioxidant vitamins (vitamins A, C and E), catalase, ceruloplasmin, glutathione peroxide, GSH and SOD protect cells against lipid oxidation (Irmak et al 2002, Meral et al 2007.…”

Section: Declaration Of Interestmentioning

confidence: 99%

Can prenatal exposure to a 900 MHz electromagnetic field affect the morphology of the spleen and thymus, and alter biomarkers of oxidative damage in 21-day-old male rats?

Hancı

Türedi

Topal

et al. 2015

Biotechnic & Histochemistry

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We investigated the effects of a 900 Megahertz (MHz) electromagnetic field (EMF), applied during the prenatal period, on the spleen and thymus of 21-day-old male rat pups. Pregnant Sprague-Dawley rats were divided into control and EMF groups. We applied 900 MHz EMF for 1 h/day to the EMF group of pregnant rats. Newborn male rat pups were removed from their mothers and sacrificed on postnatal day 21. Spleen and thymus tissues were excised and examined. Compared to the control group, thymus tissue malondialdehyde levels were significantly higher in the group exposed to EMF, while glutathione levels were significantly decreased. Increased malondialdehyde and glutathione levels were observed in splenic tissue of rats exposed to EMF, while a significant decrease occurred in superoxide dismutase values compared to controls. Transmission electron microscopy showed pathological changes in cell morphology in the thymic and splenic tissues of newborn rats exposed to EMF. Exposure to 900 MHz EMF during the prenatal period can cause pathological and biochemical changes that may compromise the development of the male rat thymus and spleen.

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Oxidative and genotoxic effects of 900MHz electromagnetic fields in the earthworm Eisenia fetida

Cited by 39 publications

References 57 publications

Searching for the Perfect Wave: The Effect of Radiofrequency Electromagnetic Fields on Cells

Searching for the Perfect Wave: The Effect of Radiofrequency Electromagnetic Fields on Cells

Electromagnetic fields at a mobile phone frequency (900 MHz) trigger the onset of general stress response along with DNA modifications in Eisenia fetida earthworms

Can prenatal exposure to a 900 MHz electromagnetic field affect the morphology of the spleen and thymus, and alter biomarkers of oxidative damage in 21-day-old male rats?

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