Oxidation state of glutathione and membrane proteins in human red cells of different age

Piccinini, Giampiero; Minetti, Giampaolo; Balduini, Cesare; Brovelli, A

doi:10.1016/0047-6374(94)01511-j

Cited by 36 publications

(26 citation statements)

References 27 publications

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“…At time zero, the density distribution and the 4.1a/ 4.1b ratio profiles were similar in control and LC samples (see Fig. 1), and the 4.1a/4.1b values increased with the density of the cell population, as expected from a typical distribution of erythrocytes of different age [12].…”

Section: Cellular Properties Of the Total Population Of Erythrocytes mentioning

confidence: 54%

“…Results obtained so far have shown that the population of circulating red cells is in fact very heterogeneous, with respect to membrane surface, cell volume, cation and water content, and cell deformability [12,13]. It is thus reasonable to suspect that subpopulations of erythrocytes of different age will acquire different cellular properties when faced with the storage conditions, and behave differently after reinfusion [14].…”

Section: Introductionmentioning

confidence: 99%

“…Since the discovery that the ratio of protein 4.1a to protein 4.1b, which are a part of the red cell membrane cytoskeleton, is correlated with erythrocyte life span in mammals [7][8][9], several authors have adopted the 4.1a/4.1b ratio as a parameter to characterize the absolute age of density-fractionated human erythrocytes [10][11][12]. Results obtained so far have shown that the population of circulating red cells is in fact very heterogeneous, with respect to membrane surface, cell volume, cation and water content, and cell deformability [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

Arduini¹,

Minetti

Ciana

et al. 2006

American J Hematol

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L-Carnitine (LC) in the preservation medium during storage of red blood cells (RBC) can improve the mean 24-hr percent recovery in vivo and increase RBC life-span after reinfusion. The purpose of the study was to investigate the differences in the biochemical properties of RBCs stored in the presence or absence of LC, and the cell-age related responses to storage conditions and to LC. RBC concentrates in saline-adenine-glucose-mannitol (SAG-M) were stored in the presence or absence of 5 mM LC at 48C for up to 8 weeks. RBC subpopulations of different densities were prepared by centrifugation on Stractan density gradient. Cells were sampled at 0, 3, 6, and 8 weeks, and hematological and cellular properties analyzed (MCV, MCHC, 4.1a/4.1b ratio as a cell age parameter, intracellular Na + and K + ). After 6 weeks, MCV of RBC stored in the presence of LC was lower than that of controls (6 weeks MCV: controls 95.4 ± 1.8 fl; LC 91.5 ± 2.0 fl; n = 6; P < 0.005). This was due to swelling of control cells, and affected mainly older RBCs. LC appeared to reduce or retard cell swelling. Among the osmotically active substances whose changes during storage could contribute to cell swelling, only intracellular Na

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Section: Cellular Properties Of the Total Population Of Erythrocytes mentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

Arduini¹,

Minetti

Ciana

et al. 2006

American J Hematol

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“…In addition, enzymatic activities decline in RBCs during ageing, so it could also be that the altered activity of GCL is only present in a subpopulation of RBCs. Previous studies by Piccinini provided evidence that although the total cell GSH was decreased with aging, there is no significant difference in its concentration in the RBCs preparations of different ages when correlated with cell water content [41].Therefore, the effect of age difference of RBCs on GSH synthesis during blood storage regimens should be evaluated further. …”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 99%

Inhibition of Glutathione Synthesis via Decreased Glucose Metabolism in Stored RBCs

Xiong

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Although red blood cells (RBCs) transfusions can be lifesaving, they are not without risk. RBCs storage is associated with the abnormal metabolism of glutathione (GSH), which may increase the risk of the oxidative damage of RBCs after transfusion. The responsible mechanisms remain unknown. Methods: We determined the L-cysteine efflux and influx by evaluating the changes of free -SH concentrations in stored RBCs. The glutamate cysteine ligase (GCL) activities and protein content in stored RBCs was determined by fluorescence assay and western blotting. In addition, the glucose metabolism enzyme activity of RBCs was measured by spectrophotometric assay under in vitro incubation conditions. Results: We found that both L-cysteine transport and GCL activity significantly declined, thereby inducing the dysfunction of GSH synthesis during blood storage, which could be attenuated by ATP supplement and DTT treatment. In addition, the glycometabolic enzyme (G6PDH, HK, PK and LDH) activity significantly decreased after 6 weeks storage. Oxidant stress-induced dysfunction in glucose metabolism was the driving force for decreased GSH synthesis during storage. Conclusion: These experimental findings reflect an underlying molecular mechanism that oxidant stress induced glucose metabolism dysfunction contribute to decreased GSH synthesis in stored RBCs.

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“…There is extensive literature on the metabolic changes that occur during cell aging (1,11,39,55,56,75,77,98,107). Changes have been reported for the antioxidant enzymes SOD1, catalase, and GPx (35).…”

Section: Rbc Antioxidant Systemmentioning

confidence: 99%

Hemoglobin Redox Reactions and Red Blood Cell Aging

Rifkind

Nagababu

2013

Antioxidants & Redox Signaling

132

112

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Significance: The physiological mechanism(s) for recognition and removal of red blood cells (RBCs) from circulation after 120 days of its lifespan is not fully understood. Many of the processes thought to be associated with the removal of RBCs involve oxidative stress. We have focused on hemoglobin (Hb) redox reactions, which is the major source of RBC oxidative stress. Recent Advances: The importance of Hb redox reactions have been shown to originate in large parts from the continuous slow autoxidation of Hb producing superoxide and its dramatic increase under hypoxic conditions. In addition, oxidative stress has been shown to be associated with redox reactions that originate from Hb reactions with nitrite and nitric oxide (NO) and the resultant formation of highly toxic peroxynitrite when NO reacts with superoxide released during Hb autoxidation. Critical Issues: The interaction of Hb, particularly under hypoxic conditions with band 3 of the RBC membrane is critical for the generating the RBC membrane changes that trigger the removal of cells from circulation. These changes include exposure of antigenic sites, increased calcium leakage into the RBC, and the resultant leakage of potassium out of the RBC causing cell shrinkage and impaired deformability. Future Directions: The need to understand the oxidative damage to specific membrane proteins that result from redox reactions occurring when Hb is bound to the membrane. Proteomic studies that can pinpoint the specific proteins damaged under different conditions will help elucidate the cellular aging processes that result in cells being removed from circulation. Antioxid. Redox Signal. 18, 2274-2283.

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Oxidation state of glutathione and membrane proteins in human red cells of different age

Cited by 36 publications

References 27 publications

Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

Cellular properties of human erythrocytes preserved in saline–adenine–glucose–mannitol in the presence of L‐carnitine

Inhibition of Glutathione Synthesis via Decreased Glucose Metabolism in Stored RBCs

Hemoglobin Redox Reactions and Red Blood Cell Aging

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