Oxidation of Sperm Nucleus in Mammals: A Physiological Necessity to Some Extent with Adverse Impacts on Oocyte and Offspring

Drevet, Joël R.; Aitken, R. John

doi:10.3390/antiox9020095

Cited by 48 publications

(35 citation statements)

References 53 publications

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“…Different studies have proven that oxidative stress represents an important issue leading to sperm cell defects such as DNA damage, protein modifications, plasma membrane damage and even epigenetic modifications that can be transmitted to the offspring [68,69]. Oxidative DNA damage in mature sperm cells is irreparable, since the nature of the DNA condensation impedes the transcription of DNA repair enzymes; therefore, the integrity of the genetic material is compromised in the male gamete [15].…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Ribas-Maynou

Yeste

Salas‐Huetos

2020

Biology

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Achieving high embryo quality following IVF and ICSI procedures is a key factor in increasing fertility outcomes in human infertile couples. While the male factor is known to underlie infertility in about 50% of cases, studies performed in human infertile couples have not been able to define the precise effect of sperm affectations upon embryo development. This lack of consistency is, in most cases, due to the heterogeneity of the results caused by the multiple male and female factors that mask the concrete effect of a given sperm parameter. These biases can be reduced with the use of animal gametes, being a good approach for basic researchers to design more homogeneous studies analyzing the specific consequences of a certain affectation. Herein, we conducted a systematic review (March 2020) that assessed the relationship between sperm oxidative stress alterations and IVF/ICSI outcomes in nonhumans mammals. The review was conducted according to PRISMA guidelines and using the MEDLINE-PubMed and EMBASE databases. Thirty articles were included: 11 performed IVF, 17 conducted ICSI, and two carried out both fertilization methods. Most articles were conducted in mouse (43%), cattle (30%) and pig models (10%). After IVF treatments, 80% of studies observed a negative effect of sperm oxidative stress on fertilization rates, and 100% of studies observed a negative effect on blastocyst rates. After ICSI treatments, a positive relationship of sperm oxidative stress with fertilization rates (75% of studies) and with blastocyst rates (83% of studies) was found. In conclusion, the present systematic review shows that sperm oxidative stress is associated with a significant reduction in fertilization rates and in vitro embryo development.

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Section: Discussionmentioning

confidence: 99%

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Ribas-Maynou

Yeste

Salas‐Huetos

2020

Biology

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“…There are a lot of second messengers reported, and the knock-in technique can be applied to them since a wide variety of genetically encoded fluorescent sensors have been developed 16 . For example, cyclic AMP, inositol 1,4,5-trisphosphate, diacylglycerol and reactive oxygen species 73,74 can be detected by their specific fluorescent sensors. In addition, the active status of proteases, kinases, and GTPases 75,76 can be visualized.…”

Section: Discussionmentioning

confidence: 99%

Cas9-mediated Genome Editing Reveals a Significant Contribution of Calcium Signaling Pathways to Anhydrobiosis in Pv11

Miyata

Fuse

Tokumoto

et al. 2020

Preprint

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Pv11 is an insect cell line established from the midge Polypedilum vanderplanki that exhibits an extreme desiccation tolerance known as anhydrobiosis. Pv11 has also an anhydrobiotic ability which is induced by trehalose treatment. Here we report the successful construction of the genome editing system for Pv11 cells and its application for identifying the signaling pathways in the anhydrobiosis. Using the Cas9-mediated gene knock-in system, we established GCaMP3-stably expressing Pv11 cells to monitor intracellular Ca2+ mobilization. Intriguingly, trehalose treatment evoked a transient increase of cytosolic Ca2+ concentration, and further experiments indicated the contribution of the calmodulin - calcineurin - NFAT pathway to the tolerance for trehalose treatment as well as the desiccation tolerance, while the calmodulin - calmodulin Kinase - CREB pathway conferred only the desiccation tolerance on Pv11 cells. Thus, our results show the critical contribution of the trehalose-induced Ca2+ surge to the anhydrobiosis and the temporal different roles of each signaling pathway.

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“…It was shown that optimal levels of ROS facilitate the production of intracellular ATP and phosphorylation of axoneme proteins, which enhance sperm motility, while low levels of ROS reduce sperm motility. Secondly, ROS (in particular H 2 O 2 ) were also more recently shown to be physiologically necessary to some extent for the post-testicular epididymal maturation of spermatozoa structures [ 16 ]. Therefore, ROS are both necessary and dangerous for sperm cells.…”

Section: Reactive Oxygen Species and Male Infertilitymentioning

confidence: 99%

“…This discrepancy was explained by the fact that the human sperm nucleus is a lot less condensed than the mouse sperm nucleus, authorizing ROS to penetrate deeper inside it, readily contacting each chromosome. This lower level of nuclear compaction of the human sperm nucleus is due to its higher content in persisting histones approximately 10 times more represented than in the mouse sperm nucleus [ 16 , 47 ]. In the mouse and the human, some special hot spots for sperm DNA oxidation were identified [ 43 , 47 ].…”

Section: Sperm Dna Oxidation a Hidden Dangermentioning

confidence: 99%

Oxidation of Sperm DNA and Male Infertility

et al. 2021

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One important reason for male infertility is oxidative stress and its destructive effects on sperm structures and functions. The particular composition of the sperm membrane, rich in polyunsaturated fatty acids, and the easy access of sperm DNA to oxidative damage due to sperm cell specific cytologic and metabolic features (no cytoplasm left and cells unable to mount stress responses) make it the cell type in metazoans most susceptible to oxidative damage. In particular, oxidative damage to the spermatozoa genome is an important issue and a cause of male infertility, usually associated with single- or double-strand paternal DNA breaks. Various methods of detecting sperm DNA fragmentation have become important diagnostic tools in the prognosis of male infertility and such assays are available in research laboratories and andrology clinics. However, to date, there is not a clear consensus in the community as to their respective prognostic value. Nevertheless, it is important to understand that the effects of oxidative stress on the sperm genome go well beyond DNA fragmentation alone. Oxidation of paternal DNA bases, particularly guanine and adenosine residues, the most sensitive residues to oxidative alteration, is the starting point for DNA damage in spermatozoa but is also a danger for the integrity of the embryo genetic material independently of sperm DNA fragmentation. Due to the lack of a spermatozoa DNA repair system and, if the egg is unable to correct the sperm oxidized bases, the risk of de novo mutation transmission to the embryo exists. These will be carried on to every cell of the future individual and its progeny. Thus, in addition to affecting the viability of the pregnancy itself, oxidation of the DNA bases in sperm could be associated with the development of conditions in young and future adults. Despite these important issues, sperm DNA base oxidation has not attracted much interest among clinicians due to the lack of simple, reliable, rapid and consensual methods of assessing this type of damage to the paternal genome. In addition to these technical issues, another reason explaining why the measurement of sperm DNA oxidation is not included in male fertility is likely to be due to the lack of strong evidence for its role in pregnancy outcome. It is, however, becoming clear that the assessment of DNA base oxidation could improve the efficiency of assisted reproductive technologies and provide important information on embryonic developmental failures and pathologies encountered in the offspring. The objective of this work is to review relevant research that has been carried out in the field of sperm DNA base oxidation and its associated genetic and epigenetic consequences.

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Oxidation of Sperm Nucleus in Mammals: A Physiological Necessity to Some Extent with Adverse Impacts on Oocyte and Offspring

Cited by 48 publications

References 53 publications

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Cas9-mediated Genome Editing Reveals a Significant Contribution of Calcium Signaling Pathways to Anhydrobiosis in Pv11

Oxidation of Sperm DNA and Male Infertility

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