Oxidation of proline by sarcosomes of the tsetse fly, Glossina morsitans

Bursell, E.; Slack, Ella

doi:10.1016/0020-1790(76)90026-3

Cited by 62 publications

(25 citation statements)

References 22 publications

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“…The data presented above are consistent with parts of the pathways proposed for partial glutamate oxidation in both Glossina morsitans (Bursell and Slack, 1976) and Leptinotarsa decemlineata (Weeda et al, 1980) and with the outline of glutamate and glutamine metabolism shown in Figure 7. They are also consistent with the route of glutamine metabolism seen in the mucosa of the rat small intestine (Hanson and Parsons, 1980).…”

Section: Summary Of Glutamine and Glutamate Metabolism In Kc Cellssupporting

confidence: 88%

Glutamine and glutamate metabolism in normal and heat shock conditions in Drosophila Kc cells: Conditions supporting glutamine synthesis maximize heat shock polypeptide expression

Sanders¹,

Kon²

1992

Journal Cellular Physiology

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We have previously reported that Drosophila Kc cells require glutamine for maximal expression of heat shock proteins in stressed conditions (Sanders and Kon: J. Cell. Physiol. 146:180-190, 1991). The mechanism of this effect has been investigated by comparing the metabolic utilization of glutamine in conditions which support hsp expression with that of glutamate in conditions where up to 100-fold less hsp is synthesized. This comparison showed that free ammonia was generated by cells incubated in the presence of glutamine in 37 degrees C (heat shock) conditions, but not at 25 degrees C, and not in the presence of glutamate in either normal or heat shock conditions. There was no difference in the amount of [14C]O2 generated from either [14C]-labeled amino acid in the tricarboxylic acid cycle, but three- to four-fold more alanine was synthesized in cells incubated in glutamine than in glutamate. Treating the cells with aminotransferase inhibitors to artificially increase NH3 release raised hsp expression in the presence of glutamate to maximal levels characteristic of glutamine. This potentiation correlated with inhibition of alanine aminotransferase. Since only NH3 production correlated with hsp expression in heat shock conditions in the presence of glutamine, and NH3 addition to glutamate also resulted in maximal hsp expression, we measured glutamine production in glutamate plus NH3 and observed net glutamine synthesis. The supposition that glutamine itself is responsible for the regulatory changes supporting maximal hsp expression was supported by the finding that the glutamine analog, 6-diazo-5-oxo-L-norleucine (DON), mimicked the effects of glutamine. We conclude that glutamine imposes regulatory changes which alter nitrogen metabolism and support hsp expression in Kc cells.

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Section: Summary Of Glutamine and Glutamate Metabolism In Kc Cellssupporting

confidence: 88%

Glutamine and glutamate metabolism in normal and heat shock conditions in Drosophila Kc cells: Conditions supporting glutamine synthesis maximize heat shock polypeptide expression

Sanders¹,

Kon²

1992

Journal Cellular Physiology

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“…However there is one difference between Colorado potato beetle and tsetse fly. BURSELL and SLACK (1976) suggested that about 20~o of proline oxidation by mitochondria of the tsetse fly flight muscle is complete, which means that ammonia is formed due to the participation of glutamate dehydrogenase. The studies with mitochondria from flight muscles of the Colorado potato beetle clearly established alanine as the only STOREY and BAILEY (1978a).…”

Section: Discussionmentioning

confidence: 99%

Proline synthesis in fat body of Leptinotarsa decemlineata

Weeda

Koopmanschap

Kort

et al. 1980

Insect Biochemistry

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“…Outstanding examples are the tsetse fly, Glossina morsitans, and the Colorado beetle, Leptinotarsa decemlineata. As shown in Table 3, Leptinotarsa flight muscle mitochondria oxidize proline at a much higher rate than pyruvate, and in Glossina flight muscles this effect is even more pronounced (Bursell and Slack, 1976) (Table 7). Tsetse fly mitochondria converted about 80~ of administered radiolabelled proline into alanine, suggesting that about 20~o of the substrate is subjected to glutamate dehy- Bursell, 1977, andWeeda et al, 1980b).…”

Section: Proline As Energy Substratementioning

confidence: 92%