Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea

Hyman, Michael R.; Page, Cynthia; Arp, Daniel J.

doi:10.1128/aem.60.8.3033-3035.1994

Cited by 51 publications

(18 citation statements)

References 15 publications

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“…It will also work with other competitive inhibitors, for instance, difluoromethane (33). However, one has to take care that the CH 3 F concentration is high enough to be effective during the entire assay period, because CH 3 F can be oxidized by both methanotrophs (37) and nitrifiers (24). From our experiments with rice plant soil it could be concluded that CH 3 F did not inhibit methane oxidation at a CH 4 /CH 3 F molar ratio of Ͻ2:1 (data not shown).…”

Section: Discussionmentioning

confidence: 99%

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Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Bodelier

Frenzel

1999

Appl Environ Microbiol

122

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Methanotrophic and nitrifying bacteria are both able to oxidize CH4 as well as NH4 +. To date it is not possible to estimate the relative contribution of methanotrophs to nitrification and that of nitrifiers to CH4 oxidation and thus to assess their roles in N and C cycling in soils and sediments. This study presents new options for discrimination between the activities of methanotrophs and nitrifiers, based on the competitive inhibitor CH3F and on recovery after inhibition with C2H2. By using rice plant soil as a model system, it was possible to selectively inactivate methanotrophs in soil slurries at a CH4/CH3F/NH4 + molar ratio of 0.1:1:18. This ratio of CH3F to NH4 + did not affect ammonia oxidation, but methane oxidation was inhibited completely. By using the same model system, it could be shown that after 24 h of exposure to C2H2 (1,000 parts per million volume), methanotrophs recovered within 24 h while nitrifiers stayed inactive for at least 3 days. This gave an “assay window” of 48 h when only methanotrophs were active. Applying both assays to model microcosms planted with rice plants demonstrated a major contribution of methanotrophs to nitrification in the rhizosphere, while the contribution of nitrifiers to CH4 oxidation was insignificant.

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Section: Discussionmentioning

confidence: 99%

“…Two approaches, based on a temporary inactivation of one of the two groups, were used. Methylfluoride, being a competitive inhibitor (24,37) of CH 4 and NH 4…”

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confidence: 99%

Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Bodelier

Frenzel

1999

Appl Environ Microbiol

122

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“…Bacterial cytochromes P450 catalyze the O-dealkylation reactions of alkyl ethers and aralkyl ethers [16][17][18]. In addition, methane monooxygenase, ammonium monooxygenase, iron-sulfur centered monooxygenase and NADH-dependent dioxygenase take part in the O-dealkylation of MTBE, dimethyl ether, diethyl ether and aralkyl ethers [14,15,[19][20][21]. Generally known is that these oxidative enzymes catalyze the incorporation of an oxygen atom from oxygen molecule to the Ca-atom of the ether bond, resulting in the formation of the unstable hemiacetal structure which spontaneously decomposes to the corresponding alcohol and aldehyde [18].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of diethyl ether degradation in Rhodococcus sp. strain DEE5151 by glutaraldehyde and ethyl vinyl ether

Kim

Engesser

2005

FEMS Microbiology Letters

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Alkyl ether-degrading Rhodococcus sp. strain DEE5151, isolated from activated sewage sludge, has an activity for the oxidation of a variety of alkyl ethers, aralkyl ethers and dibenzyl ether. The whole cell activity for diethyl ether oxidation was effectively inhibited by 2,3-dihydrofurane, ethyl vinyl ether and glutaraldehyde. Glutaraldehyde of less than 30 microM inhibited the activity by a competitive manner with the inhibition constant, K(I) of 7.07+/-1.36 microM. The inhibition type became mixed at higher glutaraldehyde concentrations >30 microM, probably due to the inactivation of the cell activity by the Schiff-base formation. Structurally analogous ethyl vinyl ether inhibited the diethyl ether oxidation activity in a mixed manner with decreasing the apparent maximum oxidation rate, v(max)(app), and increasing the apparent Michaelis-Menten constant, K(M)(app). The mixed type inhibition by ethyl vinyl ether seemed to be introduced not only by the structure similarity with diethyl ether, but also by the reactivity of the vinyl ether with cellular components in the whole cell system.

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“…Through the action of AMO, N. europaea can oxidize a number of aliphatic and aromatic compounds in addition to NH 3 . Examples of cooxidants include alkanes (17,20), alkenes (17,21), aromatics (24), ethers (18), and thioethers (22). The oxidation of these alternative substrates does not provide any known energy benefit to the cell and is dependent on NH 3 oxidation for a sustained supply of reductant.…”

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Effects of Soil on Ammonia, Ethylene, Chloroethane, and 1,1,1-Trichloroethane Oxidation by Nitrosomonas europaea

Hommes¹,

Russell²,

Bottomley

et al. 1998

Appl Environ Microbiol

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Ammonia monooxygenase (AMO) from Nitrosomonas europaeacatalyzes the oxidation of ammonia to hydroxylamine and has been shown to oxidize a variety of halogenated and nonhalogenated hydrocarbons. As part of a program focused upon extending these observations to natural systems, a study was conducted to examine the influence of soil upon the cooxidative abilities of N. europaea. Small quantities of Willamette silt loam (organic carbon content, 1.8%; cation-exchange capacity, 15 cmol/kg of soil) were suspended with N. europaea cells in a soil-slurry-type reaction mixture. The oxidations of ammonia and three different hydrocarbons (ethylene, chloroethane, and 1,1,1-trichloroethane) were compared to results for controls in which no soil was added. The soil significantly inhibited nitrite production from 10 mM ammonium by N. europaea. Inhibition resulted from a combination of ammonium adsorption onto soil colloids and the exchangeable acidity of the soil lowering the pH of the reaction mixture. These phenomena resulted in a substantial drop in the concentration of NH4 + in solution (10 to 4.5 mM) and, depending upon the pH, in a reduction in the amount of available NH3 to concentrations (8 to 80 μM) similar to the Ks value of AMO for NH3 (∼29 μM). At a fixed initial pH (7.8), the presence of soil also modified the rates of oxidation of ethylene and chloroethane and changed the concentrations at which their maximal rates of oxidation occurred. The modifying effects of soil on nitrite production and on the cooxidation of ethylene and chloroethane could be circumvented by raising the ammonium concentration in the reaction mixture from 10 to 50 mM. Soil had virtually no effect on the oxidation of 1,1,1-trichloroethane.

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Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea

Cited by 51 publications

References 15 publications

Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Inhibition of diethyl ether degradation in Rhodococcus sp. strain DEE5151 by glutaraldehyde and ethyl vinyl ether

Effects of Soil on Ammonia, Ethylene, Chloroethane, and 1,1,1-Trichloroethane Oxidation by Nitrosomonas europaea

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Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea

Cited by 51 publications

References 15 publications

Contribution of Methanotrophic and Nitrifying Bacteria to CH 4 and NH 4 + Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Contribution of Methanotrophic and Nitrifying Bacteria to CH 4 and NH 4 + Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Inhibition of diethyl ether degradation in Rhodococcus sp. strain DEE5151 by glutaraldehyde and ethyl vinyl ether

Effects of Soil on Ammonia, Ethylene, Chloroethane, and 1,1,1-Trichloroethane Oxidation by Nitrosomonas europaea

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Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination

Contribution of Methanotrophic and Nitrifying Bacteria to CH ₄ and NH ₄ ⁺ Oxidation in the Rhizosphere of Rice Plants as Determined by New Methods of Discrimination