Oxidation of l-glucose by a Pseudomonad

Sasajima, Ken-ichi; Sinskey, Anthony J.

doi:10.1016/0005-2744(79)90232-8

Cited by 21 publications

(16 citation statements)

References 26 publications

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“…Of the very few species documented to use L-glucose for growth, two species, one bacterium and one fungal species, live endo-/epi-phytic lifestyles (Sasajima and Sinskey, 1979; Shimizu et al, 2012). L-glucose biosynthesis in a plant system was characterized in the 1970's (Barber, 1971).…”

Section: Discussionmentioning

confidence: 99%

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

Nelson

Khraiwesh

et al. 2017

eLife

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To investigate the phenomic and genomic traits that allow green algae to survive in deserts, we characterized a ubiquitous species, Chloroidium sp. UTEX 3007, which we isolated from multiple locations in the United Arab Emirates (UAE). Metabolomic analyses of Chloroidium sp. UTEX 3007 indicated that the alga accumulates a broad range of carbon sources, including several desiccation tolerance-promoting sugars and unusually large stores of palmitate. Growth assays revealed capacities to grow in salinities from zero to 60 g/L and to grow heterotrophically on >40 distinct carbon sources. Assembly and annotation of genomic reads yielded a 52.5 Mbp genome with 8153 functionally annotated genes. Comparison with other sequenced green algae revealed unique protein families involved in osmotic stress tolerance and saccharide metabolism that support phenomic studies. Our results reveal the robust and flexible biology utilized by a green alga to successfully inhabit a desert coastline.DOI: http://dx.doi.org/10.7554/eLife.25783.001

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Section: Discussionmentioning

confidence: 99%

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

Nelson

Khraiwesh

et al. 2017

eLife

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“…For screening of L-glucose-utilizing microorganisms, L-glucose minimal medium (L-Glc MM, 2 10 mM NH 4 Cl, 10 mM potassium phosphate, pH 7.0, 10 mM MgSO 4 6 Mo 7 O 24 ⅐4H 2 O) containing 14 mM L-glucose; L-glucose was separately sterilized by filtration and added to the medium) was used, and for L-glucose dehydrogenase (L-GDH) purification, D-galactose minimal medium (20 mM NH 4 Cl, 20 mM potassium phosphate, pH 7.0, 10 mM MgSO 4 , 10 mM KCl, 0.2% (v/v) Hutner's trace element solution, 0.1% yeast extract containing 28 mM D-galactose; D-galactose was sterilized separately by filtration) was used.…”

Section: Methodsmentioning

confidence: 99%

An l-glucose Catabolic Pathway in Paracoccus Species 43P

Shimizu

Takaya

Nakamura

2012

Journal of Biological Chemistry

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Background: L-Glucose, the enantiomer of D-glucose, was believed not to be utilized by any organisms. Results: An L-glucose-utilizing bacterium was isolated, and its L-glucose catabolic pathway was identified genetically and enzymatically. Conclusion: L-Glucose was utilized via a novel pathway to pyruvate and D-glyceraldehyde 3-phosphate. Significance: This might lead to an understanding of homochirality in sugar metabolism.

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“…The high-yield conversion of D-glucose into methyl 2,3-0-isopropylidene-P-D-gulofuranoside (5) had been shown (26) to proceed through the intermediacy of methyl 2,3:5,6-di-0-isopropylidene-P-D-gulofuranoside. This selectivity in hydrolysis was noted (27) also in the preparation of methyl 2,3-0-isopropylidene-a-D-mannofuranoside from methyl 2,3 : 5,6-di-0-isopropylidene-a-D-mannofuranoside However, methyl 2,3 : 5,6-di-0-isopropylidene-@-D-mannofuranoside was much more sensitive to acid and selective hydrolysis was unattainable (27).…”

Section: Resultsmentioning

confidence: 99%

“…Although there have been claims that L-glucose occurs as a component of certain complex carbohydrates (3), the evidence is inconclusive as yet. Moreover, most studies of the metabolism of L-glucose by microorganisms have indicated that it is not metabolized (4); an enzyme catalyzing the oxidation of L-glucose has been isolated from cell-free extracts of Pseudomoizns caryophylii I F 0 1 3694 (5). These data suggested that certain of the enantiomers of naturally occurring sugars may be safe, effective, non-nutritive sweeteners.…”

Section: Introductionmentioning

confidence: 99%