Oxidation of glyceraldehyde-3-phosphate dehydrogenase decreases sperm motility

Elkina, Yu. L.; Atroshchenko, Mikhail; Брагина, Е. Е.; Muronetz, Vladimir I.; Schmalhausen, E.V.

doi:10.1134/s0006297911020143

Cited by 32 publications

(21 citation statements)

References 24 publications

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“…The high relative transcript level of GAPDH in stored sperm is consistent with the need for protein synthesis during storage. Sperm-specific isoforms of GAPDH have been identified in mammalian sperm, these have not been found in insect sperm28. However, it is possible that similar sperm-specific isoforms do exist in honey bee sperm which should be a subject for future study.…”

Section: Discussionmentioning

confidence: 96%

“…However, it is possible that similar sperm-specific isoforms do exist in honey bee sperm which should be a subject for future study. ROS has been shown to reduce GAPDH activity in sperm from other species28. Although it appears that it may be possible to replace enzymes such as GAPDH during storage, the low oxygen environment found in the spermatheca is ideal for maintaining GAPDH function without ROS damage over a long period of time.…”

Section: Discussionmentioning

confidence: 99%

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Insights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera)

Paynter

Millar

Welch

et al. 2017

Sci Rep

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Honeybee males produce ejaculates consisting of large numbers of high quality sperm. Because queens never re-mate after a single mating episode early in life, sperm are stored in a specialised organ for years but the proximate mechanisms underlying this key physiological adaptation are unknown. We quantified energy metabolism in honeybee sperm and show that the glycolytic metabolite glyceraldehyde-3-phosphate (GA3P) is a key substrate for honeybee sperm survival and energy production. This reliance on non-aerobic energy metabolism in stored sperm was further supported by our findings of very low levels of oxygen inside the spermatheca. Expression of GA3P dehydrogenase (GAPDH), the enzyme involved in catabolism of GA3P, was significantly higher in stored compared to ejaculated sperm. Therefore, long-term sperm storage seems facilitated by the maintenance of non-aerobic energy production, the need for only the ATP-producing steps of glycolysis and by avoiding sperm damage resulting from ROS production. We also confirm that honeybee sperm is capable of aerobic metabolism, which predominates in ejaculated sperm while they compete for access to the spermatheca, but is suppressed during storage. Consequently, the remarkable reproductive traits of honeybees are proximately achieved by differential usage of energy production pathways to maximise competitiveness and minimise damage of sperm.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Insights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera)

Paynter

Millar

Welch

et al. 2017

Sci Rep

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“…Glycolysis thus seems to have an important role in energy production. Evidence of this theory comes from studies of GAPD2 by Elkina et al 16. who demonstrated an enzyme activity 2.5–3 times lower in hypokinetic samples than in normokinetic samples while tissue localization studies17 revealed GAPD2 in the testicles and epididymis.…”

Section: Discussionmentioning

confidence: 99%

Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa

et al. 2017

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It has been suggested that the energy required for sperm motility is produced by oxidative phosphorylation while glycolysis seems to be an important source for ATP transmission along the flagellum. Some studies have investigated the chemical and kinetic properties of the enzyme glyceraldehyde 3-phosphate dehydrogenase to identify any changes in the regulation of glycolysis and sperm motility. In contrast, there are few studies analyzing the genetic basis of hypokinesis. For this reason, we investigated the glyceraldehyde 3-phosphate dehydrogenase gene in human sperm to evaluate whether asthenozoospermia was correlated with any changes in its expression. Semen examination and glyceraldehyde 3-phosphate dehydrogenase gene expression studies were carried out on 116 semen samples divided into two groups – Group A consisted of 58 normokinetic samples and Group B of 58 hypokinetic samples. Total RNA was extracted from spermatozoa, and real-time PCR quantification of mRNA was carried out using specific primers and probes. The expression profiles for the Groups A and B were very similar. The mean delta Ct was as follows – Group A, 5.79 ± 1.04; Group B, 5.47 ± 1.27. Our study shows that in human sperm, there is no difference in glyceraldehyde 3-phosphate dehydrogenase gene expression between samples with impaired motility and samples with normal kinetics. We believe that this study could help in the understanding of the molecular mechanisms of sperm kinetics, suggesting that hypomotility may be due to a possible posttranscriptional impairment of the control mechanism, such as mRNA splicing, or to posttranslational changes.

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“…Research targeting spermiogenesis and differentiation [100,101], maturation of sperm [102,103], or factors inhibiting sperm motility [104][105][106][107], identified promising targets for nonhormonal contraception in men, and a few may enter into clinical testing stage during the current decade. None of these approaches will be available to the prescriber before another decade and long-term studies.…”

Section: Genomics and Proteomics: New Contraceptive Targetsmentioning

confidence: 98%

Applying emerging science to contraception research: implications for the clinic

Sitruk-Ware

Nath

2014

Expert Review of Endocrinology & Metabolism

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Emerging science will make an important contribution towards the development of improved contraceptives. While long-acting reversible contraceptives remain the most effective method, new user-controlled, mid-acting methods will avoid the need for procedures requiring trained providers. Contraceptives combined with other agents may bring additional health benefits, such as dual protection against both pregnancy and sexually transmitted infections. Emerging research areas in proteomics allowed the discovery of new reproductive targets that may lead to non-hormonal contraceptives for both men and women. Current research objectives include the improvement of existing contraceptive methods, as well as discovery of new materials able to deliver new molecules more specifically to their target without systemic actions.

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Oxidation of glyceraldehyde-3-phosphate dehydrogenase decreases sperm motility

Cited by 32 publications

References 24 publications

Insights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera)

Insights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera)

Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa

Applying emerging science to contraception research: implications for the clinic

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